Resolution-enhanced Fourier ptychographic microscopy based on high-numerical-aperture illuminations

Abstract High-resolution and wide field-of-view (FOV) microscopic imaging plays a central role in diverse applications such as high-throughput screening and digital pathology. However, conventional microscopes face inherent trade-offs between the spatial resolution and FOV, which are fundamental lim...

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Autores principales:	Jiasong Sun, Chao Zuo, Liang Zhang, Qian Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9fe608106ab4451d839f430ad4e6d381
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spelling	oai:doaj.org-article:9fe608106ab4451d839f430ad4e6d3812021-12-02T15:05:49ZResolution-enhanced Fourier ptychographic microscopy based on high-numerical-aperture illuminations10.1038/s41598-017-01346-72045-2322https://doaj.org/article/9fe608106ab4451d839f430ad4e6d3812017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01346-7https://doaj.org/toc/2045-2322Abstract High-resolution and wide field-of-view (FOV) microscopic imaging plays a central role in diverse applications such as high-throughput screening and digital pathology. However, conventional microscopes face inherent trade-offs between the spatial resolution and FOV, which are fundamental limited by the space-bandwidth product (SBP) of the optical system. The resolution-FOV tradeoff can be effectively decoupled in Fourier ptychography microscopy (FPM), however, to date, the effective imaging NA achievable with a typical FPM system is still limited to the range of 0.4–0.7. Herein, we report, for the first time, a high-NA illumination based resolution-enhanced FPM (REFPM) platform, in which a LED-array-based digital oil-immersion condenser is used to create high-angle programmable plane-wave illuminations, endowing a 10×, 0.4 NA objective lens with final effective imaging performance of 1.6 NA. With REFPM, we present the highest-resolution results with a unprecedented half-pitch resolution of 154 nm at a wavelength of 435 nm across a wide FOV of 2.34 mm2, corresponding to an SBP of 98.5 megapixels (~50 times higher than that of the conventional incoherent microscope with the same resolution). Our work provides an important step of FPM towards high-resolution large-NA imaging applications, generating comparable resolution performance but significantly broadening the FOV of conventional oil-immersion microscopes.Jiasong SunChao ZuoLiang ZhangQian ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiasong Sun Chao Zuo Liang Zhang Qian Chen Resolution-enhanced Fourier ptychographic microscopy based on high-numerical-aperture illuminations
description	Abstract High-resolution and wide field-of-view (FOV) microscopic imaging plays a central role in diverse applications such as high-throughput screening and digital pathology. However, conventional microscopes face inherent trade-offs between the spatial resolution and FOV, which are fundamental limited by the space-bandwidth product (SBP) of the optical system. The resolution-FOV tradeoff can be effectively decoupled in Fourier ptychography microscopy (FPM), however, to date, the effective imaging NA achievable with a typical FPM system is still limited to the range of 0.4–0.7. Herein, we report, for the first time, a high-NA illumination based resolution-enhanced FPM (REFPM) platform, in which a LED-array-based digital oil-immersion condenser is used to create high-angle programmable plane-wave illuminations, endowing a 10×, 0.4 NA objective lens with final effective imaging performance of 1.6 NA. With REFPM, we present the highest-resolution results with a unprecedented half-pitch resolution of 154 nm at a wavelength of 435 nm across a wide FOV of 2.34 mm2, corresponding to an SBP of 98.5 megapixels (~50 times higher than that of the conventional incoherent microscope with the same resolution). Our work provides an important step of FPM towards high-resolution large-NA imaging applications, generating comparable resolution performance but significantly broadening the FOV of conventional oil-immersion microscopes.
format	article
author	Jiasong Sun Chao Zuo Liang Zhang Qian Chen
author_facet	Jiasong Sun Chao Zuo Liang Zhang Qian Chen
author_sort	Jiasong Sun
title	Resolution-enhanced Fourier ptychographic microscopy based on high-numerical-aperture illuminations
title_short	Resolution-enhanced Fourier ptychographic microscopy based on high-numerical-aperture illuminations
title_full	Resolution-enhanced Fourier ptychographic microscopy based on high-numerical-aperture illuminations
title_fullStr	Resolution-enhanced Fourier ptychographic microscopy based on high-numerical-aperture illuminations
title_full_unstemmed	Resolution-enhanced Fourier ptychographic microscopy based on high-numerical-aperture illuminations
title_sort	resolution-enhanced fourier ptychographic microscopy based on high-numerical-aperture illuminations
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9fe608106ab4451d839f430ad4e6d381
work_keys_str_mv	AT jiasongsun resolutionenhancedfourierptychographicmicroscopybasedonhighnumericalapertureilluminations AT chaozuo resolutionenhancedfourierptychographicmicroscopybasedonhighnumericalapertureilluminations AT liangzhang resolutionenhancedfourierptychographicmicroscopybasedonhighnumericalapertureilluminations AT qianchen resolutionenhancedfourierptychographicmicroscopybasedonhighnumericalapertureilluminations
_version_	1718388721646043136

Resolution-enhanced Fourier ptychographic microscopy based on high-numerical-aperture illuminations

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