Rescuing cholinergic neurons from apoptotic degeneration by targeting of serotonin modulator- and apolipoprotein E-conjugated liposomes to the hippocampus

Yung-Chih Kuo, Yin-Jung Lee Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China Abstract: β-Amyloid (Aβ)-targeting liposomes (LIP) with surface serotonin modulator (SM) and apolipoprotein E (ApoE) were utilized to facilitate the...

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Autores principales:	Kuo YC, Lee YJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	liposome nerve growth factor Alzheimer's disease blood-brain barrier serotonin modulator apolipoprotein E Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9fe644fb9a154719b7256ea898b7542c
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spelling	oai:doaj.org-article:9fe644fb9a154719b7256ea898b7542c2021-12-02T05:02:16ZRescuing cholinergic neurons from apoptotic degeneration by targeting of serotonin modulator- and apolipoprotein E-conjugated liposomes to the hippocampus1178-2013https://doaj.org/article/9fe644fb9a154719b7256ea898b7542c2016-12-01T00:00:00Zhttps://www.dovepress.com/rescuing-cholinergic-neurons-from-apoptotic-degeneration-by-targeting--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yung-Chih Kuo, Yin-Jung Lee Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China Abstract: β-Amyloid (Aβ)-targeting liposomes (LIP) with surface serotonin modulator (SM) and apolipoprotein E (ApoE) were utilized to facilitate the delivery of nerve growth factor (NGF) across the blood–brain barrier (BBB) for neuroprotection in the hippocampus. The therapeutic efficacy of SM- and ApoE-grafted LIP carrying NGF (NGF-SM-ApoE-LIP) was assessed by an in vitro Alzheimer’s disease (AD) model of degenerated SK-N-MC cells and an in vivo AD model of Aβ-insulted Wistar rats. The experimental evidences revealed that the modified SM and ApoE on the surface of LIP increased the permeation of NGF across the BBB without serious damage to structural integrity of tight junction. When compared with free NGF, NGF-SM-ApoE-LIP upregulated the expression of phosphorylated neurotrophic tyrosine kinase receptor type 1 on cholinergic neurons and significantly improved their survival. In addition, NGF-SM-ApoE-LIP could reduce the secretion of acetylcholinesterase and malondialdehyde and rescue hippocampal neurons from apoptosis in rat brains. The synergistic effect of SM and ApoE is promising in the induction of NGF to inhibit the neurotoxicity of Aβ and NGF-SM-ApoE-LIP can be a potent antiapoptotic pharmacotherapy for clinical care of patients with AD. Keywords: Alzheimer’s disease, blood–brain barrier, serotonin modulator, apolipoprotein E, nerve growth factor, liposomeKuo YCLee YJDove Medical Pressarticleliposomenerve growth factorAlzheimer's diseaseblood-brain barrierserotonin modulatorapolipoprotein EMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6809-6824 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	liposome nerve growth factor Alzheimer's disease blood-brain barrier serotonin modulator apolipoprotein E Medicine (General) R5-920
spellingShingle	liposome nerve growth factor Alzheimer's disease blood-brain barrier serotonin modulator apolipoprotein E Medicine (General) R5-920 Kuo YC Lee YJ Rescuing cholinergic neurons from apoptotic degeneration by targeting of serotonin modulator- and apolipoprotein E-conjugated liposomes to the hippocampus
description	Yung-Chih Kuo, Yin-Jung Lee Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China Abstract: β-Amyloid (Aβ)-targeting liposomes (LIP) with surface serotonin modulator (SM) and apolipoprotein E (ApoE) were utilized to facilitate the delivery of nerve growth factor (NGF) across the blood–brain barrier (BBB) for neuroprotection in the hippocampus. The therapeutic efficacy of SM- and ApoE-grafted LIP carrying NGF (NGF-SM-ApoE-LIP) was assessed by an in vitro Alzheimer’s disease (AD) model of degenerated SK-N-MC cells and an in vivo AD model of Aβ-insulted Wistar rats. The experimental evidences revealed that the modified SM and ApoE on the surface of LIP increased the permeation of NGF across the BBB without serious damage to structural integrity of tight junction. When compared with free NGF, NGF-SM-ApoE-LIP upregulated the expression of phosphorylated neurotrophic tyrosine kinase receptor type 1 on cholinergic neurons and significantly improved their survival. In addition, NGF-SM-ApoE-LIP could reduce the secretion of acetylcholinesterase and malondialdehyde and rescue hippocampal neurons from apoptosis in rat brains. The synergistic effect of SM and ApoE is promising in the induction of NGF to inhibit the neurotoxicity of Aβ and NGF-SM-ApoE-LIP can be a potent antiapoptotic pharmacotherapy for clinical care of patients with AD. Keywords: Alzheimer’s disease, blood–brain barrier, serotonin modulator, apolipoprotein E, nerve growth factor, liposome
format	article
author	Kuo YC Lee YJ
author_facet	Kuo YC Lee YJ
author_sort	Kuo YC
title	Rescuing cholinergic neurons from apoptotic degeneration by targeting of serotonin modulator- and apolipoprotein E-conjugated liposomes to the hippocampus
title_short	Rescuing cholinergic neurons from apoptotic degeneration by targeting of serotonin modulator- and apolipoprotein E-conjugated liposomes to the hippocampus
title_full	Rescuing cholinergic neurons from apoptotic degeneration by targeting of serotonin modulator- and apolipoprotein E-conjugated liposomes to the hippocampus
title_fullStr	Rescuing cholinergic neurons from apoptotic degeneration by targeting of serotonin modulator- and apolipoprotein E-conjugated liposomes to the hippocampus
title_full_unstemmed	Rescuing cholinergic neurons from apoptotic degeneration by targeting of serotonin modulator- and apolipoprotein E-conjugated liposomes to the hippocampus
title_sort	rescuing cholinergic neurons from apoptotic degeneration by targeting of serotonin modulator- and apolipoprotein e-conjugated liposomes to the hippocampus
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/9fe644fb9a154719b7256ea898b7542c
work_keys_str_mv	AT kuoyc rescuingcholinergicneuronsfromapoptoticdegenerationbytargetingofserotoninmodulatorandapolipoproteineconjugatedliposomestothehippocampus AT leeyj rescuingcholinergicneuronsfromapoptoticdegenerationbytargetingofserotoninmodulatorandapolipoproteineconjugatedliposomestothehippocampus
_version_	1718400782049476608

Rescuing cholinergic neurons from apoptotic degeneration by targeting of serotonin modulator- and apolipoprotein E-conjugated liposomes to the hippocampus

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