Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5

Abstract During the establishment of pregnancy, extravillous trophoblast (EVT) must invade into the uterine decidua to facilitate decidual artery remodelling to create the placental blood supply. The local decidual environment is thought to regulate trophoblast invasion, however these interactions a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	E. M. Menkhorst, M. L. Van Sinderen, K. Rainczuk, C. Cuman, A. Winship, E. Dimitriadis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9feba91aa68c41ba9162df6811a20734
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9feba91aa68c41ba9162df6811a20734
record_format	dspace
spelling	oai:doaj.org-article:9feba91aa68c41ba9162df6811a207342021-12-02T11:41:20ZInvasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX510.1038/s41598-017-05947-02045-2322https://doaj.org/article/9feba91aa68c41ba9162df6811a207342017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05947-0https://doaj.org/toc/2045-2322Abstract During the establishment of pregnancy, extravillous trophoblast (EVT) must invade into the uterine decidua to facilitate decidual artery remodelling to create the placental blood supply. The local decidual environment is thought to regulate trophoblast invasion, however these interactions are poorly defined in humans. Recent evidence in women suggests impaired decidualization is associated with miscarriage and preeclampsia. Primary human endometrial stromal cells (HESC) and first trimester extravillous trophoblast (EVTs) were used to assess the effect of EVT-secreted factors on HESC decidualization, adhesion, proliferation and migration. We determined the role of profilin (PFN)1, an EVT-secreted factor, on HESC function and identified a downstream target of PFN1. EVT-secreted factors induced HESC decidualization and enhanced decidualized HESC adhesion, proliferation and migration. Recombinant PFN1 enhanced methoxyprogesterone acetate-induced HESC decidualization and proliferation. PFN1 down-regulated the expression of lipoxygenase arachidonate 5-lipoxygenase (ALOX5) in HESC and THP-1 macrophages. ALOX5 localised to decidual cells and CD68+macrophages in 1st trimester decidua. This study demonstrated that EVT secretions, including PFN1, enhanced HESC decidualization and motility. This study has identified a new pathway that facilitates appropriate decidualization during the establishment of pregnancy.E. M. MenkhorstM. L. Van SinderenK. RainczukC. CumanA. WinshipE. DimitriadisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q E. M. Menkhorst M. L. Van Sinderen K. Rainczuk C. Cuman A. Winship E. Dimitriadis Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
description	Abstract During the establishment of pregnancy, extravillous trophoblast (EVT) must invade into the uterine decidua to facilitate decidual artery remodelling to create the placental blood supply. The local decidual environment is thought to regulate trophoblast invasion, however these interactions are poorly defined in humans. Recent evidence in women suggests impaired decidualization is associated with miscarriage and preeclampsia. Primary human endometrial stromal cells (HESC) and first trimester extravillous trophoblast (EVTs) were used to assess the effect of EVT-secreted factors on HESC decidualization, adhesion, proliferation and migration. We determined the role of profilin (PFN)1, an EVT-secreted factor, on HESC function and identified a downstream target of PFN1. EVT-secreted factors induced HESC decidualization and enhanced decidualized HESC adhesion, proliferation and migration. Recombinant PFN1 enhanced methoxyprogesterone acetate-induced HESC decidualization and proliferation. PFN1 down-regulated the expression of lipoxygenase arachidonate 5-lipoxygenase (ALOX5) in HESC and THP-1 macrophages. ALOX5 localised to decidual cells and CD68+macrophages in 1st trimester decidua. This study demonstrated that EVT secretions, including PFN1, enhanced HESC decidualization and motility. This study has identified a new pathway that facilitates appropriate decidualization during the establishment of pregnancy.
format	article
author	E. M. Menkhorst M. L. Van Sinderen K. Rainczuk C. Cuman A. Winship E. Dimitriadis
author_facet	E. M. Menkhorst M. L. Van Sinderen K. Rainczuk C. Cuman A. Winship E. Dimitriadis
author_sort	E. M. Menkhorst
title	Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
title_short	Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
title_full	Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
title_fullStr	Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
title_full_unstemmed	Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
title_sort	invasive trophoblast promote stromal fibroblast decidualization via profilin 1 and alox5
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9feba91aa68c41ba9162df6811a20734
work_keys_str_mv	AT emmenkhorst invasivetrophoblastpromotestromalfibroblastdecidualizationviaprofilin1andalox5 AT mlvansinderen invasivetrophoblastpromotestromalfibroblastdecidualizationviaprofilin1andalox5 AT krainczuk invasivetrophoblastpromotestromalfibroblastdecidualizationviaprofilin1andalox5 AT ccuman invasivetrophoblastpromotestromalfibroblastdecidualizationviaprofilin1andalox5 AT awinship invasivetrophoblastpromotestromalfibroblastdecidualizationviaprofilin1andalox5 AT edimitriadis invasivetrophoblastpromotestromalfibroblastdecidualizationviaprofilin1andalox5
_version_	1718395415865327616

Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5

Ejemplares similares