Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5

Abstract During the establishment of pregnancy, extravillous trophoblast (EVT) must invade into the uterine decidua to facilitate decidual artery remodelling to create the placental blood supply. The local decidual environment is thought to regulate trophoblast invasion, however these interactions a...

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Autores principales: E. M. Menkhorst, M. L. Van Sinderen, K. Rainczuk, C. Cuman, A. Winship, E. Dimitriadis
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/9feba91aa68c41ba9162df6811a20734
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spelling oai:doaj.org-article:9feba91aa68c41ba9162df6811a207342021-12-02T11:41:20ZInvasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX510.1038/s41598-017-05947-02045-2322https://doaj.org/article/9feba91aa68c41ba9162df6811a207342017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05947-0https://doaj.org/toc/2045-2322Abstract During the establishment of pregnancy, extravillous trophoblast (EVT) must invade into the uterine decidua to facilitate decidual artery remodelling to create the placental blood supply. The local decidual environment is thought to regulate trophoblast invasion, however these interactions are poorly defined in humans. Recent evidence in women suggests impaired decidualization is associated with miscarriage and preeclampsia. Primary human endometrial stromal cells (HESC) and first trimester extravillous trophoblast (EVTs) were used to assess the effect of EVT-secreted factors on HESC decidualization, adhesion, proliferation and migration. We determined the role of profilin (PFN)1, an EVT-secreted factor, on HESC function and identified a downstream target of PFN1. EVT-secreted factors induced HESC decidualization and enhanced decidualized HESC adhesion, proliferation and migration. Recombinant PFN1 enhanced methoxyprogesterone acetate-induced HESC decidualization and proliferation. PFN1 down-regulated the expression of lipoxygenase arachidonate 5-lipoxygenase (ALOX5) in HESC and THP-1 macrophages. ALOX5 localised to decidual cells and CD68+macrophages in 1st trimester decidua. This study demonstrated that EVT secretions, including PFN1, enhanced HESC decidualization and motility. This study has identified a new pathway that facilitates appropriate decidualization during the establishment of pregnancy.E. M. MenkhorstM. L. Van SinderenK. RainczukC. CumanA. WinshipE. DimitriadisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
E. M. Menkhorst
M. L. Van Sinderen
K. Rainczuk
C. Cuman
A. Winship
E. Dimitriadis
Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
description Abstract During the establishment of pregnancy, extravillous trophoblast (EVT) must invade into the uterine decidua to facilitate decidual artery remodelling to create the placental blood supply. The local decidual environment is thought to regulate trophoblast invasion, however these interactions are poorly defined in humans. Recent evidence in women suggests impaired decidualization is associated with miscarriage and preeclampsia. Primary human endometrial stromal cells (HESC) and first trimester extravillous trophoblast (EVTs) were used to assess the effect of EVT-secreted factors on HESC decidualization, adhesion, proliferation and migration. We determined the role of profilin (PFN)1, an EVT-secreted factor, on HESC function and identified a downstream target of PFN1. EVT-secreted factors induced HESC decidualization and enhanced decidualized HESC adhesion, proliferation and migration. Recombinant PFN1 enhanced methoxyprogesterone acetate-induced HESC decidualization and proliferation. PFN1 down-regulated the expression of lipoxygenase arachidonate 5-lipoxygenase (ALOX5) in HESC and THP-1 macrophages. ALOX5 localised to decidual cells and CD68+macrophages in 1st trimester decidua. This study demonstrated that EVT secretions, including PFN1, enhanced HESC decidualization and motility. This study has identified a new pathway that facilitates appropriate decidualization during the establishment of pregnancy.
format article
author E. M. Menkhorst
M. L. Van Sinderen
K. Rainczuk
C. Cuman
A. Winship
E. Dimitriadis
author_facet E. M. Menkhorst
M. L. Van Sinderen
K. Rainczuk
C. Cuman
A. Winship
E. Dimitriadis
author_sort E. M. Menkhorst
title Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
title_short Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
title_full Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
title_fullStr Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
title_full_unstemmed Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5
title_sort invasive trophoblast promote stromal fibroblast decidualization via profilin 1 and alox5
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/9feba91aa68c41ba9162df6811a20734
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