Overexpression of cytokinin dehydrogenase genes in barley (Hordeum vulgare cv. Golden Promise) fundamentally affects morphology and fertility.
Barley is one of the most important cereal crops grown worldwide. It has numerous applications, but its utility could potentially be extended by genetically manipulating its hormonal balances. To explore some of this potential we identified gene families of cytokinin dehydrogenases (CKX) and isopent...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9febfe315548485abcb197946c5cf887 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:9febfe315548485abcb197946c5cf887 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:9febfe315548485abcb197946c5cf8872021-11-18T08:46:09ZOverexpression of cytokinin dehydrogenase genes in barley (Hordeum vulgare cv. Golden Promise) fundamentally affects morphology and fertility.1932-620310.1371/journal.pone.0079029https://doaj.org/article/9febfe315548485abcb197946c5cf8872013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24260147/?tool=EBIhttps://doaj.org/toc/1932-6203Barley is one of the most important cereal crops grown worldwide. It has numerous applications, but its utility could potentially be extended by genetically manipulating its hormonal balances. To explore some of this potential we identified gene families of cytokinin dehydrogenases (CKX) and isopentenyl transferases, enzymes that respectively irreversibly degrade and synthesize cytokinin (CK) plant hormones, in the raw sequenced barley genome. We then examined their spatial and temporal expression patterns by immunostaining and qPCR. Two CKX-specific antibodies, anti-HvCKX1 and anti-HvCKX9, predominantly detect proteins in the aleurone layer of maturing grains and leaf vasculature, respectively. In addition, two selected CKX genes were used for stable, Agrobacterium tumefaciens-mediated transformation of the barley cultivar Golden Promise. The results show that constitutive overexpression of CKX causes morphological changes in barley plants and prevents their transition to flowering. In all independent transgenic lines roots proliferated more rapidly and root-to-shoot ratios were higher than in wild-type plants. Only one transgenic line, overexpressing CKX under the control of a promoter from a phosphate transporter gene, which is expressed more strongly in root tissue than in aerial parts, yielded progeny. Analysis of several T1-generation plants indicates that plants tend to compensate for effects of the transgene and restore CK homeostasis later during development. Depleted CK levels during early phases of development are restored by down-regulation of endogenous CKX genes and reinforced de novo biosynthesis of CKs.Katarína MrízováEva JiskrováŠárka VyroubalováOndřej NovákLudmila OhnoutkováHana PospíšilováIvo FrébortWendy A HarwoodPetr GaluszkaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79029 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Katarína Mrízová Eva Jiskrová Šárka Vyroubalová Ondřej Novák Ludmila Ohnoutková Hana Pospíšilová Ivo Frébort Wendy A Harwood Petr Galuszka Overexpression of cytokinin dehydrogenase genes in barley (Hordeum vulgare cv. Golden Promise) fundamentally affects morphology and fertility. |
| description |
Barley is one of the most important cereal crops grown worldwide. It has numerous applications, but its utility could potentially be extended by genetically manipulating its hormonal balances. To explore some of this potential we identified gene families of cytokinin dehydrogenases (CKX) and isopentenyl transferases, enzymes that respectively irreversibly degrade and synthesize cytokinin (CK) plant hormones, in the raw sequenced barley genome. We then examined their spatial and temporal expression patterns by immunostaining and qPCR. Two CKX-specific antibodies, anti-HvCKX1 and anti-HvCKX9, predominantly detect proteins in the aleurone layer of maturing grains and leaf vasculature, respectively. In addition, two selected CKX genes were used for stable, Agrobacterium tumefaciens-mediated transformation of the barley cultivar Golden Promise. The results show that constitutive overexpression of CKX causes morphological changes in barley plants and prevents their transition to flowering. In all independent transgenic lines roots proliferated more rapidly and root-to-shoot ratios were higher than in wild-type plants. Only one transgenic line, overexpressing CKX under the control of a promoter from a phosphate transporter gene, which is expressed more strongly in root tissue than in aerial parts, yielded progeny. Analysis of several T1-generation plants indicates that plants tend to compensate for effects of the transgene and restore CK homeostasis later during development. Depleted CK levels during early phases of development are restored by down-regulation of endogenous CKX genes and reinforced de novo biosynthesis of CKs. |
| format |
article |
| author |
Katarína Mrízová Eva Jiskrová Šárka Vyroubalová Ondřej Novák Ludmila Ohnoutková Hana Pospíšilová Ivo Frébort Wendy A Harwood Petr Galuszka |
| author_facet |
Katarína Mrízová Eva Jiskrová Šárka Vyroubalová Ondřej Novák Ludmila Ohnoutková Hana Pospíšilová Ivo Frébort Wendy A Harwood Petr Galuszka |
| author_sort |
Katarína Mrízová |
| title |
Overexpression of cytokinin dehydrogenase genes in barley (Hordeum vulgare cv. Golden Promise) fundamentally affects morphology and fertility. |
| title_short |
Overexpression of cytokinin dehydrogenase genes in barley (Hordeum vulgare cv. Golden Promise) fundamentally affects morphology and fertility. |
| title_full |
Overexpression of cytokinin dehydrogenase genes in barley (Hordeum vulgare cv. Golden Promise) fundamentally affects morphology and fertility. |
| title_fullStr |
Overexpression of cytokinin dehydrogenase genes in barley (Hordeum vulgare cv. Golden Promise) fundamentally affects morphology and fertility. |
| title_full_unstemmed |
Overexpression of cytokinin dehydrogenase genes in barley (Hordeum vulgare cv. Golden Promise) fundamentally affects morphology and fertility. |
| title_sort |
overexpression of cytokinin dehydrogenase genes in barley (hordeum vulgare cv. golden promise) fundamentally affects morphology and fertility. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/9febfe315548485abcb197946c5cf887 |
| work_keys_str_mv |
AT katarinamrizova overexpressionofcytokinindehydrogenasegenesinbarleyhordeumvulgarecvgoldenpromisefundamentallyaffectsmorphologyandfertility AT evajiskrova overexpressionofcytokinindehydrogenasegenesinbarleyhordeumvulgarecvgoldenpromisefundamentallyaffectsmorphologyandfertility AT sarkavyroubalova overexpressionofcytokinindehydrogenasegenesinbarleyhordeumvulgarecvgoldenpromisefundamentallyaffectsmorphologyandfertility AT ondrejnovak overexpressionofcytokinindehydrogenasegenesinbarleyhordeumvulgarecvgoldenpromisefundamentallyaffectsmorphologyandfertility AT ludmilaohnoutkova overexpressionofcytokinindehydrogenasegenesinbarleyhordeumvulgarecvgoldenpromisefundamentallyaffectsmorphologyandfertility AT hanapospisilova overexpressionofcytokinindehydrogenasegenesinbarleyhordeumvulgarecvgoldenpromisefundamentallyaffectsmorphologyandfertility AT ivofrebort overexpressionofcytokinindehydrogenasegenesinbarleyhordeumvulgarecvgoldenpromisefundamentallyaffectsmorphologyandfertility AT wendyaharwood overexpressionofcytokinindehydrogenasegenesinbarleyhordeumvulgarecvgoldenpromisefundamentallyaffectsmorphologyandfertility AT petrgaluszka overexpressionofcytokinindehydrogenasegenesinbarleyhordeumvulgarecvgoldenpromisefundamentallyaffectsmorphologyandfertility |
| _version_ |
1718421338791608320 |