ISCOM-like Nanoparticles Formulated with <i>Quillaja brasiliensis</i> Saponins Are Promising Adjuvants for Seasonal Influenza Vaccines

Vaccination is the most effective public health intervention to prevent influenza infections, which are responsible for an important burden of respiratory illnesses and deaths each year. Currently, licensed influenza vaccines are mostly split inactivated, although in order to achieve higher efficacy...

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Autores principales:	Mariana Rivera-Patron, María Moreno, Mariana Baz, Paulo M. Roehe, Samuel P. Cibulski, Fernando Silveira
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adjuvants <i>Quillaja brasiliensis</i> ISCOMs influenza virus dose-sparing effect Medicine R
Acceso en línea:	https://doaj.org/article/9fedb75c42254620bbf05504f97bb74d
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spelling	oai:doaj.org-article:9fedb75c42254620bbf05504f97bb74d2021-11-25T19:11:32ZISCOM-like Nanoparticles Formulated with <i>Quillaja brasiliensis</i> Saponins Are Promising Adjuvants for Seasonal Influenza Vaccines10.3390/vaccines91113502076-393Xhttps://doaj.org/article/9fedb75c42254620bbf05504f97bb74d2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1350https://doaj.org/toc/2076-393XVaccination is the most effective public health intervention to prevent influenza infections, which are responsible for an important burden of respiratory illnesses and deaths each year. Currently, licensed influenza vaccines are mostly split inactivated, although in order to achieve higher efficacy rates, some influenza vaccines contain adjuvants. Although split-inactivated vaccines induce mostly humoral responses, tailoring mucosal and cellular immune responses is crucial for preventing influenza infections. <i>Quillaja brasiliensis</i> saponin-based adjuvants, including ISCOM-like nanoparticles formulated with the QB-90 saponin fraction (IQB90), have been studied in preclinical models for more than a decade and have been demonstrated to induce strong humoral and cellular immune responses towards several viral antigens. Herein, we demonstrate that a split-inactivated IQB90 adjuvanted influenza vaccine triggered a protective immune response, stronger than that induced by a commercial unadjuvanted vaccine, when applied either by the subcutaneous or the intranasal route. Moreover, we reveal that this novel adjuvant confers up to a ten-fold dose-sparing effect, which could be crucial for pandemic preparedness. Last but not least, we assessed the role of caspase-1/11 in the generation of the immune response triggered by the IQB90 adjuvanted influenza vaccine in a mouse model and found that the cellular-mediated immune response triggered by the IQB90-Flu relies, at least in part, on a mechanism involving the casp-1/11 pathway but not the humoral response elicited by this formulation.Mariana Rivera-PatronMaría MorenoMariana BazPaulo M. RoeheSamuel P. CibulskiFernando SilveiraMDPI AGarticleadjuvants<i>Quillaja brasiliensis</i>ISCOMsinfluenza virusdose-sparing effectMedicineRENVaccines, Vol 9, Iss 1350, p 1350 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adjuvants <i>Quillaja brasiliensis</i> ISCOMs influenza virus dose-sparing effect Medicine R
spellingShingle	adjuvants <i>Quillaja brasiliensis</i> ISCOMs influenza virus dose-sparing effect Medicine R Mariana Rivera-Patron María Moreno Mariana Baz Paulo M. Roehe Samuel P. Cibulski Fernando Silveira ISCOM-like Nanoparticles Formulated with <i>Quillaja brasiliensis</i> Saponins Are Promising Adjuvants for Seasonal Influenza Vaccines
description	Vaccination is the most effective public health intervention to prevent influenza infections, which are responsible for an important burden of respiratory illnesses and deaths each year. Currently, licensed influenza vaccines are mostly split inactivated, although in order to achieve higher efficacy rates, some influenza vaccines contain adjuvants. Although split-inactivated vaccines induce mostly humoral responses, tailoring mucosal and cellular immune responses is crucial for preventing influenza infections. <i>Quillaja brasiliensis</i> saponin-based adjuvants, including ISCOM-like nanoparticles formulated with the QB-90 saponin fraction (IQB90), have been studied in preclinical models for more than a decade and have been demonstrated to induce strong humoral and cellular immune responses towards several viral antigens. Herein, we demonstrate that a split-inactivated IQB90 adjuvanted influenza vaccine triggered a protective immune response, stronger than that induced by a commercial unadjuvanted vaccine, when applied either by the subcutaneous or the intranasal route. Moreover, we reveal that this novel adjuvant confers up to a ten-fold dose-sparing effect, which could be crucial for pandemic preparedness. Last but not least, we assessed the role of caspase-1/11 in the generation of the immune response triggered by the IQB90 adjuvanted influenza vaccine in a mouse model and found that the cellular-mediated immune response triggered by the IQB90-Flu relies, at least in part, on a mechanism involving the casp-1/11 pathway but not the humoral response elicited by this formulation.
format	article
author	Mariana Rivera-Patron María Moreno Mariana Baz Paulo M. Roehe Samuel P. Cibulski Fernando Silveira
author_facet	Mariana Rivera-Patron María Moreno Mariana Baz Paulo M. Roehe Samuel P. Cibulski Fernando Silveira
author_sort	Mariana Rivera-Patron
title	ISCOM-like Nanoparticles Formulated with <i>Quillaja brasiliensis</i> Saponins Are Promising Adjuvants for Seasonal Influenza Vaccines
title_short	ISCOM-like Nanoparticles Formulated with <i>Quillaja brasiliensis</i> Saponins Are Promising Adjuvants for Seasonal Influenza Vaccines
title_full	ISCOM-like Nanoparticles Formulated with <i>Quillaja brasiliensis</i> Saponins Are Promising Adjuvants for Seasonal Influenza Vaccines
title_fullStr	ISCOM-like Nanoparticles Formulated with <i>Quillaja brasiliensis</i> Saponins Are Promising Adjuvants for Seasonal Influenza Vaccines
title_full_unstemmed	ISCOM-like Nanoparticles Formulated with <i>Quillaja brasiliensis</i> Saponins Are Promising Adjuvants for Seasonal Influenza Vaccines
title_sort	iscom-like nanoparticles formulated with <i>quillaja brasiliensis</i> saponins are promising adjuvants for seasonal influenza vaccines
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9fedb75c42254620bbf05504f97bb74d
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ISCOM-like Nanoparticles Formulated with <i>Quillaja brasiliensis</i> Saponins Are Promising Adjuvants for Seasonal Influenza Vaccines

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