Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds

Abstract The short survival time of transplanted adipose-derived mesenchymal stem cells (ASCs) is a problem for skin wound healing. Transplantation after the formation of cellular spheroids has been investigated as a promising method for prolonging cellular survival. However, there have been technic...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hisato Nagano, Yoshitaka Suematsu, Megumi Takuma, Shimpo Aoki, Ayano Satoh, Eiji Takayama, Manabu Kinoshita, Yuji Morimoto, Shinji Takeoka, Toshinori Fujie, Tomoharu Kiyosawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9fef994d41934c7bac14eee927a6c7f2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9fef994d41934c7bac14eee927a6c7f2
record_format	dspace
spelling	oai:doaj.org-article:9fef994d41934c7bac14eee927a6c7f22021-12-02T18:30:51ZEnhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds10.1038/s41598-021-93642-62045-2322https://doaj.org/article/9fef994d41934c7bac14eee927a6c7f22021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93642-6https://doaj.org/toc/2045-2322Abstract The short survival time of transplanted adipose-derived mesenchymal stem cells (ASCs) is a problem for skin wound healing. Transplantation after the formation of cellular spheroids has been investigated as a promising method for prolonging cellular survival. However, there have been technical restrictions for transplantation of spheroids in clinical practice. Here, we show an effective method for transplantation of ASC spheroids onto skin wounds in order to efficiently cure refractory ulcers. To assist anchoring of spheroids onto skin wounds, we used a 120-nm-thick free-standing film (nanosheet) that has a highly adhesive property. Bioluminescence imaging showed that ASC spheroids carried by the nanosheet survived for 14 days, which is about two-times longer than that previously reported. Wounds treated with a nanosheet carrying ASC spheroids were 4-times smaller than untreated wounds on day 14. This method for transplantation of spheroids could be applied to cell therapy for various refractory skin wounds.Hisato NaganoYoshitaka SuematsuMegumi TakumaShimpo AokiAyano SatohEiji TakayamaManabu KinoshitaYuji MorimotoShinji TakeokaToshinori FujieTomoharu KiyosawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hisato Nagano Yoshitaka Suematsu Megumi Takuma Shimpo Aoki Ayano Satoh Eiji Takayama Manabu Kinoshita Yuji Morimoto Shinji Takeoka Toshinori Fujie Tomoharu Kiyosawa Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds
description	Abstract The short survival time of transplanted adipose-derived mesenchymal stem cells (ASCs) is a problem for skin wound healing. Transplantation after the formation of cellular spheroids has been investigated as a promising method for prolonging cellular survival. However, there have been technical restrictions for transplantation of spheroids in clinical practice. Here, we show an effective method for transplantation of ASC spheroids onto skin wounds in order to efficiently cure refractory ulcers. To assist anchoring of spheroids onto skin wounds, we used a 120-nm-thick free-standing film (nanosheet) that has a highly adhesive property. Bioluminescence imaging showed that ASC spheroids carried by the nanosheet survived for 14 days, which is about two-times longer than that previously reported. Wounds treated with a nanosheet carrying ASC spheroids were 4-times smaller than untreated wounds on day 14. This method for transplantation of spheroids could be applied to cell therapy for various refractory skin wounds.
format	article
author	Hisato Nagano Yoshitaka Suematsu Megumi Takuma Shimpo Aoki Ayano Satoh Eiji Takayama Manabu Kinoshita Yuji Morimoto Shinji Takeoka Toshinori Fujie Tomoharu Kiyosawa
author_facet	Hisato Nagano Yoshitaka Suematsu Megumi Takuma Shimpo Aoki Ayano Satoh Eiji Takayama Manabu Kinoshita Yuji Morimoto Shinji Takeoka Toshinori Fujie Tomoharu Kiyosawa
author_sort	Hisato Nagano
title	Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds
title_short	Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds
title_full	Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds
title_fullStr	Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds
title_full_unstemmed	Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds
title_sort	enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9fef994d41934c7bac14eee927a6c7f2
work_keys_str_mv	AT hisatonagano enhancedcellularengraftmentofadiposederivedmesenchymalstemcellspheroidsbyusingnanosheetsasscaffolds AT yoshitakasuematsu enhancedcellularengraftmentofadiposederivedmesenchymalstemcellspheroidsbyusingnanosheetsasscaffolds AT megumitakuma enhancedcellularengraftmentofadiposederivedmesenchymalstemcellspheroidsbyusingnanosheetsasscaffolds AT shimpoaoki enhancedcellularengraftmentofadiposederivedmesenchymalstemcellspheroidsbyusingnanosheetsasscaffolds AT ayanosatoh enhancedcellularengraftmentofadiposederivedmesenchymalstemcellspheroidsbyusingnanosheetsasscaffolds AT eijitakayama enhancedcellularengraftmentofadiposederivedmesenchymalstemcellspheroidsbyusingnanosheetsasscaffolds AT manabukinoshita enhancedcellularengraftmentofadiposederivedmesenchymalstemcellspheroidsbyusingnanosheetsasscaffolds AT yujimorimoto enhancedcellularengraftmentofadiposederivedmesenchymalstemcellspheroidsbyusingnanosheetsasscaffolds AT shinjitakeoka enhancedcellularengraftmentofadiposederivedmesenchymalstemcellspheroidsbyusingnanosheetsasscaffolds AT toshinorifujie enhancedcellularengraftmentofadiposederivedmesenchymalstemcellspheroidsbyusingnanosheetsasscaffolds AT tomoharukiyosawa enhancedcellularengraftmentofadiposederivedmesenchymalstemcellspheroidsbyusingnanosheetsasscaffolds
_version_	1718377997214416896

Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds

Ejemplares similares