Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus

Geminiviruses infect many crop plants, and are limiting factors for vegetable crop production. Begomoviruses (Geminiviridae) cause typical symptoms of leaf curling and puckering in nightshade (Solanum nigrum), a seasonal weed in Bihar, India. To investigate if nightshade was an intermediate host for...

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Autores principales:	Mohammad Ansar, Aniruddha Kumar Agnihotri, Tushar Ranjan, Monika Karn, Srinivasaraghavan A, Ravi Ranjan Kumar, Arun Prasad Bhagat
Formato:	article
Lenguaje:	EN
Publicado:	Firenze University Press 2021
Materias:	Begomovirus genetic diversity leaf curl Botany QK1-989
Acceso en línea:	https://doaj.org/article/9ff9e2b4d9c8478c819f1bea3fbb34e8
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spelling	oai:doaj.org-article:9ff9e2b4d9c8478c819f1bea3fbb34e82021-11-16T09:30:32ZNightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus10.36253/phyto-127450031-94651593-2095https://doaj.org/article/9ff9e2b4d9c8478c819f1bea3fbb34e82021-11-01T00:00:00Zhttps://oajournals.fupress.net/index.php/pm/article/view/12745https://doaj.org/toc/0031-9465https://doaj.org/toc/1593-2095Geminiviruses infect many crop plants, and are limiting factors for vegetable crop production. Begomoviruses (Geminiviridae) cause typical symptoms of leaf curling and puckering in nightshade (Solanum nigrum), a seasonal weed in Bihar, India. To investigate if nightshade was an intermediate host for begomovirus, virus DNA was extracted and characterized. The DNA-A of the virus yielded 2737 nt and DNA-B yielded 2706 nt. The intergenic region (IR) showed a conserved nonanucleotide sequence that potentially forms a stem-loop structure. The genomic sequence of DNA-A shared 94% identity with that of Tomato leaf curl New Delhi virus (ToLCNDV)-ivy gourd isolate. However, the sequence of DNA-B showed 95% identity with a bitter gourd isolate. PCR-based detection revealed the presence ToLCNDV in bottle gourd, pumpkin, sponge gourd, and bitter gourd. The IR sequences of the viruses isolated from these cucurbits and tomato were 100% identical. Whitefly-mediated transmission of the virus to cucurbits and tomato from nightshade was also demonstrated. These results indicate that nightshade may act as reservoir of ToLCNDV, and is involved in developing epidemics in cucurbit species. The strain of ToLCNDV has probably adapted from solanaceous to cucurbitaceous hosts. This is the ﬁrst report of ToLCNDV infecting nightshade in India, highlighting this virus as a possible cause of disease epidemics in economically important cucurbits. Mohammad AnsarAniruddha Kumar AgnihotriTushar RanjanMonika KarnSrinivasaraghavan ARavi Ranjan KumarArun Prasad BhagatFirenze University PressarticleBegomovirusgenetic diversityleaf curlBotanyQK1-989ENPhytopathologia Mediterranea, Vol 60, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Begomovirus genetic diversity leaf curl Botany QK1-989
spellingShingle	Begomovirus genetic diversity leaf curl Botany QK1-989 Mohammad Ansar Aniruddha Kumar Agnihotri Tushar Ranjan Monika Karn Srinivasaraghavan A Ravi Ranjan Kumar Arun Prasad Bhagat Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus
description	Geminiviruses infect many crop plants, and are limiting factors for vegetable crop production. Begomoviruses (Geminiviridae) cause typical symptoms of leaf curling and puckering in nightshade (Solanum nigrum), a seasonal weed in Bihar, India. To investigate if nightshade was an intermediate host for begomovirus, virus DNA was extracted and characterized. The DNA-A of the virus yielded 2737 nt and DNA-B yielded 2706 nt. The intergenic region (IR) showed a conserved nonanucleotide sequence that potentially forms a stem-loop structure. The genomic sequence of DNA-A shared 94% identity with that of Tomato leaf curl New Delhi virus (ToLCNDV)-ivy gourd isolate. However, the sequence of DNA-B showed 95% identity with a bitter gourd isolate. PCR-based detection revealed the presence ToLCNDV in bottle gourd, pumpkin, sponge gourd, and bitter gourd. The IR sequences of the viruses isolated from these cucurbits and tomato were 100% identical. Whitefly-mediated transmission of the virus to cucurbits and tomato from nightshade was also demonstrated. These results indicate that nightshade may act as reservoir of ToLCNDV, and is involved in developing epidemics in cucurbit species. The strain of ToLCNDV has probably adapted from solanaceous to cucurbitaceous hosts. This is the ﬁrst report of ToLCNDV infecting nightshade in India, highlighting this virus as a possible cause of disease epidemics in economically important cucurbits.
format	article
author	Mohammad Ansar Aniruddha Kumar Agnihotri Tushar Ranjan Monika Karn Srinivasaraghavan A Ravi Ranjan Kumar Arun Prasad Bhagat
author_facet	Mohammad Ansar Aniruddha Kumar Agnihotri Tushar Ranjan Monika Karn Srinivasaraghavan A Ravi Ranjan Kumar Arun Prasad Bhagat
author_sort	Mohammad Ansar
title	Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus
title_short	Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus
title_full	Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus
title_fullStr	Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus
title_full_unstemmed	Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus
title_sort	nightshade (solanum nigrum), an intermediate host between tomato and cucurbits of tomato leaf curl new delhi virus
publisher	Firenze University Press
publishDate	2021
url	https://doaj.org/article/9ff9e2b4d9c8478c819f1bea3fbb34e8
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Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus

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