Roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.

<h4>Background</h4>Cerebellar cortical molecular layer interneurons (MLIs) play essential roles in sensory information processing by the cerebellar cortex. However, recent experimental and modeling results are questioning traditional roles for molecular layer inhibition in the cerebellum...

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Autores principales:	Chun-Ping Chu, Yan-Hua Bing, Heng Liu, De-Lai Qiu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9ffcad3c5cae45d5a7e8b3aef8fdff42
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spelling	oai:doaj.org-article:9ffcad3c5cae45d5a7e8b3aef8fdff422021-11-18T07:18:13ZRoles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.1932-620310.1371/journal.pone.0037031https://doaj.org/article/9ffcad3c5cae45d5a7e8b3aef8fdff422012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22623975/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Cerebellar cortical molecular layer interneurons (MLIs) play essential roles in sensory information processing by the cerebellar cortex. However, recent experimental and modeling results are questioning traditional roles for molecular layer inhibition in the cerebellum.<h4>Methods and main results</h4>Synaptic responses of MLIs and Purkinje cells (PCs), evoked by air-puff stimulation of the ipsilateral whisker pad were recorded from cerebellar cortex Crus II in urethane-anesthetized ICR mice by in vivo whole-cell patch-clamp recording techniques. Under current-clamp (I = 0), air-puff stimuli were found to primarily produce inhibition in PCs. In MLIs, this stimulus evoked spike firing regardless of whether they made basket-type synaptic connections or not. However, MLIs not making basket-type synaptic connections had higher rates of background activity and also generated spontaneous spike-lets. Under voltage-clamp conditions, excitatory postsynaptic currents (EPSCs) were recorded in MLIs, although the predominant response of recorded PCs was an inhibitory postsynaptic potential (IPSP). The latencies of EPSCs were similar for all MLIs, but the time course and amplitude of EPSCs varied with depth in the molecular layer. The highest amplitude, shortest duration EPSCs were recorded from MLIs deep in the molecular layer, which also made basket-type synaptic connections. Comparing MLI to PC responses, time to peak of PC IPSP was significantly slower than MLI recorded EPSCs. Blocking GABA(A) receptors uncovered larger EPSCs in PCs whose time to peak, half-width and 10-90% rising time were also significantly slower than in MLIs. Biocytin labeling indicated that the MLIs (but not PCs) are dye-coupled.<h4>Conclusions</h4>These findings indicate that tactile face stimulation evokes rapid excitation in MLIs and inhibition occurring at later latencies in PCs in mouse cerebellar cortex Crus II. These results support previous suggestions that the lack of parallel fiber driven PC activity is due to the effect of MLI inhibition.Chun-Ping ChuYan-Hua BingHeng LiuDe-Lai QiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37031 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chun-Ping Chu Yan-Hua Bing Heng Liu De-Lai Qiu Roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.
description	<h4>Background</h4>Cerebellar cortical molecular layer interneurons (MLIs) play essential roles in sensory information processing by the cerebellar cortex. However, recent experimental and modeling results are questioning traditional roles for molecular layer inhibition in the cerebellum.<h4>Methods and main results</h4>Synaptic responses of MLIs and Purkinje cells (PCs), evoked by air-puff stimulation of the ipsilateral whisker pad were recorded from cerebellar cortex Crus II in urethane-anesthetized ICR mice by in vivo whole-cell patch-clamp recording techniques. Under current-clamp (I = 0), air-puff stimuli were found to primarily produce inhibition in PCs. In MLIs, this stimulus evoked spike firing regardless of whether they made basket-type synaptic connections or not. However, MLIs not making basket-type synaptic connections had higher rates of background activity and also generated spontaneous spike-lets. Under voltage-clamp conditions, excitatory postsynaptic currents (EPSCs) were recorded in MLIs, although the predominant response of recorded PCs was an inhibitory postsynaptic potential (IPSP). The latencies of EPSCs were similar for all MLIs, but the time course and amplitude of EPSCs varied with depth in the molecular layer. The highest amplitude, shortest duration EPSCs were recorded from MLIs deep in the molecular layer, which also made basket-type synaptic connections. Comparing MLI to PC responses, time to peak of PC IPSP was significantly slower than MLI recorded EPSCs. Blocking GABA(A) receptors uncovered larger EPSCs in PCs whose time to peak, half-width and 10-90% rising time were also significantly slower than in MLIs. Biocytin labeling indicated that the MLIs (but not PCs) are dye-coupled.<h4>Conclusions</h4>These findings indicate that tactile face stimulation evokes rapid excitation in MLIs and inhibition occurring at later latencies in PCs in mouse cerebellar cortex Crus II. These results support previous suggestions that the lack of parallel fiber driven PC activity is due to the effect of MLI inhibition.
format	article
author	Chun-Ping Chu Yan-Hua Bing Heng Liu De-Lai Qiu
author_facet	Chun-Ping Chu Yan-Hua Bing Heng Liu De-Lai Qiu
author_sort	Chun-Ping Chu
title	Roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.
title_short	Roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.
title_full	Roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.
title_fullStr	Roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.
title_full_unstemmed	Roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.
title_sort	roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex crus ii in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9ffcad3c5cae45d5a7e8b3aef8fdff42
work_keys_str_mv	AT chunpingchu rolesofmolecularlayerinterneuronsinsensoryinformationprocessinginmousecerebellarcortexcrusiiinvivo AT yanhuabing rolesofmolecularlayerinterneuronsinsensoryinformationprocessinginmousecerebellarcortexcrusiiinvivo AT hengliu rolesofmolecularlayerinterneuronsinsensoryinformationprocessinginmousecerebellarcortexcrusiiinvivo AT delaiqiu rolesofmolecularlayerinterneuronsinsensoryinformationprocessinginmousecerebellarcortexcrusiiinvivo
_version_	1718423682195390464

Roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.

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