Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease

Chagas disease is a debilitating and often fatal pathology resulting from infection by the protozoan parasite <i>Trypanosoma cruzi</i>. In its recommendations, the World Health Organization states that the diagnosis of <i>T. cruzi</i> infection is usually based on the detecti...

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Autores principales:	Jean-Yves Brossas, Ballering Griselda, Margarita Bisio, Jeremy Guihenneuc, Julián Ernesto Nicolás Gulin, Stéphane Jauréguiberry, François-Xavier Lescure, Arnaud Fekkar, Dominique Mazier, Jaime Altcheh, Luc Paris
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	chagas disease diagnostic <i>Trypanosoma cruzi</i> Medicine R
Acceso en línea:	https://doaj.org/article/9ffd31c262d243a8bf344d4701317d77
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spelling	oai:doaj.org-article:9ffd31c262d243a8bf344d4701317d772021-11-25T18:38:23ZEvaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease10.3390/pathogens101114552076-0817https://doaj.org/article/9ffd31c262d243a8bf344d4701317d772021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1455https://doaj.org/toc/2076-0817Chagas disease is a debilitating and often fatal pathology resulting from infection by the protozoan parasite <i>Trypanosoma cruzi</i>. In its recommendations, the World Health Organization states that the diagnosis of <i>T. cruzi</i> infection is usually based on the detection of antibodies against <i>T. cruzi</i> antigens and performed with two methodologically different assays. An inconclusive result can be resolved with a third “confirmatory” assay. The objective of this article is to evaluate the effectiveness of the Chagas Western Blot IgG assay (LDBio Diagnostics, Lyon, France) as a confirmatory serologic test. The Chagas Western Blot IgG assay was performed with native antigens derived from a <i>T. cruzi</i> strain of the TcVI genotype. Retrospective sera were provided by two parasitology laboratories (France and Argentina). The sensitivity, specificity, positive predictive value and negative predictive value of the Chagas blot were all 100% in our sera collection. The Chagas blot is an easy and qualitative method for the diagnosis of Chagas disease, with results in less than 2 h. This immunoblot has potential as a supplemental test for the confirmation of the presence of antibodies against <i>T. cruzi</i> in serum specimens. Nonetheless, the very good initial results presented here will need to be confirmed in larger studies.Jean-Yves BrossasBallering GriseldaMargarita BisioJeremy GuihenneucJulián Ernesto Nicolás GulinStéphane JauréguiberryFrançois-Xavier LescureArnaud FekkarDominique MazierJaime AltchehLuc ParisMDPI AGarticlechagas diseasediagnostic<i>Trypanosoma cruzi</i>MedicineRENPathogens, Vol 10, Iss 1455, p 1455 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chagas disease diagnostic <i>Trypanosoma cruzi</i> Medicine R
spellingShingle	chagas disease diagnostic <i>Trypanosoma cruzi</i> Medicine R Jean-Yves Brossas Ballering Griselda Margarita Bisio Jeremy Guihenneuc Julián Ernesto Nicolás Gulin Stéphane Jauréguiberry François-Xavier Lescure Arnaud Fekkar Dominique Mazier Jaime Altcheh Luc Paris Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease
description	Chagas disease is a debilitating and often fatal pathology resulting from infection by the protozoan parasite <i>Trypanosoma cruzi</i>. In its recommendations, the World Health Organization states that the diagnosis of <i>T. cruzi</i> infection is usually based on the detection of antibodies against <i>T. cruzi</i> antigens and performed with two methodologically different assays. An inconclusive result can be resolved with a third “confirmatory” assay. The objective of this article is to evaluate the effectiveness of the Chagas Western Blot IgG assay (LDBio Diagnostics, Lyon, France) as a confirmatory serologic test. The Chagas Western Blot IgG assay was performed with native antigens derived from a <i>T. cruzi</i> strain of the TcVI genotype. Retrospective sera were provided by two parasitology laboratories (France and Argentina). The sensitivity, specificity, positive predictive value and negative predictive value of the Chagas blot were all 100% in our sera collection. The Chagas blot is an easy and qualitative method for the diagnosis of Chagas disease, with results in less than 2 h. This immunoblot has potential as a supplemental test for the confirmation of the presence of antibodies against <i>T. cruzi</i> in serum specimens. Nonetheless, the very good initial results presented here will need to be confirmed in larger studies.
format	article
author	Jean-Yves Brossas Ballering Griselda Margarita Bisio Jeremy Guihenneuc Julián Ernesto Nicolás Gulin Stéphane Jauréguiberry François-Xavier Lescure Arnaud Fekkar Dominique Mazier Jaime Altcheh Luc Paris
author_facet	Jean-Yves Brossas Ballering Griselda Margarita Bisio Jeremy Guihenneuc Julián Ernesto Nicolás Gulin Stéphane Jauréguiberry François-Xavier Lescure Arnaud Fekkar Dominique Mazier Jaime Altcheh Luc Paris
author_sort	Jean-Yves Brossas
title	Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease
title_short	Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease
title_full	Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease
title_fullStr	Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease
title_full_unstemmed	Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease
title_sort	evaluation of the chagas western blot igg assay for the diagnosis of chagas disease
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9ffd31c262d243a8bf344d4701317d77
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_version_	1718410907585871872

Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease

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