Linking the morphological and metabolomic response of Lactuca sativa L exposed to emerging contaminants using GC × GC-MS and chemometric tools

Abstract The occurrence of contaminants of emerging concern (CECs) in irrigation waters (up to low μg L−1) and irrigated crops (ng g−1 in dry weight) has been reported, but the linkage between plant morphological changes and plant metabolomic response has not yet been addressed. In this study, a non...

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Autores principales:	Carlos Hurtado, Hadi Parastar, Víctor Matamoros, Benjamín Piña, Romà Tauler, Josep M. Bayona
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a001985013184133b4e12c7eee88fa89
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spelling	oai:doaj.org-article:a001985013184133b4e12c7eee88fa892021-12-02T15:04:59ZLinking the morphological and metabolomic response of Lactuca sativa L exposed to emerging contaminants using GC × GC-MS and chemometric tools10.1038/s41598-017-06773-02045-2322https://doaj.org/article/a001985013184133b4e12c7eee88fa892017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06773-0https://doaj.org/toc/2045-2322Abstract The occurrence of contaminants of emerging concern (CECs) in irrigation waters (up to low μg L−1) and irrigated crops (ng g−1 in dry weight) has been reported, but the linkage between plant morphological changes and plant metabolomic response has not yet been addressed. In this study, a non-targeted metabolomic analysis was performed on lettuce (Lactuca sativa L) exposed to 11 CECs (pharmaceuticals, personal care products, anticorrosive agents and surfactants) by irrigation. The plants were watered with different CEC concentrations (0–50 µg L−1) for 34 days under controlled conditions and then harvested, extracted, derivatised and analysed by comprehensive two-dimensional gas chromatography coupled to a time-of-flight mass spectrometer (GC × GC-TOFMS). The resulting raw data were analysed using multivariate curve resolution (MCR) and partial least squares (PLS) methods. The metabolic response indicates that exposure to CECs at environmentally relevant concentrations (0.05 µg L−1) can cause significant metabolic alterations in plants (carbohydrate metabolism, the citric acid cycle, pentose phosphate pathway and glutathione pathway) linked to changes in morphological parameters (leaf height, stem width) and chlorophyll content.Carlos HurtadoHadi ParastarVíctor MatamorosBenjamín PiñaRomà TaulerJosep M. BayonaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carlos Hurtado Hadi Parastar Víctor Matamoros Benjamín Piña Romà Tauler Josep M. Bayona Linking the morphological and metabolomic response of Lactuca sativa L exposed to emerging contaminants using GC × GC-MS and chemometric tools
description	Abstract The occurrence of contaminants of emerging concern (CECs) in irrigation waters (up to low μg L−1) and irrigated crops (ng g−1 in dry weight) has been reported, but the linkage between plant morphological changes and plant metabolomic response has not yet been addressed. In this study, a non-targeted metabolomic analysis was performed on lettuce (Lactuca sativa L) exposed to 11 CECs (pharmaceuticals, personal care products, anticorrosive agents and surfactants) by irrigation. The plants were watered with different CEC concentrations (0–50 µg L−1) for 34 days under controlled conditions and then harvested, extracted, derivatised and analysed by comprehensive two-dimensional gas chromatography coupled to a time-of-flight mass spectrometer (GC × GC-TOFMS). The resulting raw data were analysed using multivariate curve resolution (MCR) and partial least squares (PLS) methods. The metabolic response indicates that exposure to CECs at environmentally relevant concentrations (0.05 µg L−1) can cause significant metabolic alterations in plants (carbohydrate metabolism, the citric acid cycle, pentose phosphate pathway and glutathione pathway) linked to changes in morphological parameters (leaf height, stem width) and chlorophyll content.
format	article
author	Carlos Hurtado Hadi Parastar Víctor Matamoros Benjamín Piña Romà Tauler Josep M. Bayona
author_facet	Carlos Hurtado Hadi Parastar Víctor Matamoros Benjamín Piña Romà Tauler Josep M. Bayona
author_sort	Carlos Hurtado
title	Linking the morphological and metabolomic response of Lactuca sativa L exposed to emerging contaminants using GC × GC-MS and chemometric tools
title_short	Linking the morphological and metabolomic response of Lactuca sativa L exposed to emerging contaminants using GC × GC-MS and chemometric tools
title_full	Linking the morphological and metabolomic response of Lactuca sativa L exposed to emerging contaminants using GC × GC-MS and chemometric tools
title_fullStr	Linking the morphological and metabolomic response of Lactuca sativa L exposed to emerging contaminants using GC × GC-MS and chemometric tools
title_full_unstemmed	Linking the morphological and metabolomic response of Lactuca sativa L exposed to emerging contaminants using GC × GC-MS and chemometric tools
title_sort	linking the morphological and metabolomic response of lactuca sativa l exposed to emerging contaminants using gc × gc-ms and chemometric tools
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a001985013184133b4e12c7eee88fa89
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Linking the morphological and metabolomic response of Lactuca sativa L exposed to emerging contaminants using GC × GC-MS and chemometric tools

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