Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore Germination and Attenuated Local and Systemic Inflammation

Abstract Clinical management of Clostridium difficile infection is still far from satisfactory as bacterial spores are resistant to many chemical agents and physical treatments. Certain types of nanoparticles have been demonstrated to exhibit anti-microbial efficacy even in multi-drug resistance bac...

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Autores principales:	Wei-Ting Lee, Ya-Na Wu, Yi-Hsuan Chen, Shang-Rung Wu, Tsai-Miao Shih, Tsung-Ju Li, Li-Xing Yang, Chen-Sheng Yeh, Pei-Jane Tsai, Dar-Bin Shieh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a00e268fa83d43cbb45283c6def3f567
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spelling	oai:doaj.org-article:a00e268fa83d43cbb45283c6def3f5672021-12-02T15:05:54ZOctahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore Germination and Attenuated Local and Systemic Inflammation10.1038/s41598-017-08387-y2045-2322https://doaj.org/article/a00e268fa83d43cbb45283c6def3f5672017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08387-yhttps://doaj.org/toc/2045-2322Abstract Clinical management of Clostridium difficile infection is still far from satisfactory as bacterial spores are resistant to many chemical agents and physical treatments. Certain types of nanoparticles have been demonstrated to exhibit anti-microbial efficacy even in multi-drug resistance bacteria. However, most of these studies failed to show biocompatibility to the mammalian host cells and no study has revealed in vivo efficacy in C. difficile infection animal models. The spores treated with 500 µg/mL Fe3-δO4 nanoparticles for 20 minutes, 64% of the spores were inhibited from transforming into vegetative cells, which was close to the results of the sodium hypochlorite-treated positive control. By cryo-electron micro-tomography, we demonstrated that Fe3-δO4 nanoparticles bind on spore surfaces and reduce the dipicolinic acid (DPA) released by the spores. In a C. difficile infection animal model, the inflammatory level triple decreased in mice with colonic C. difficile spores treated with Fe3-δO4 nanoparticles. Histopathological analysis showed a decreased intense neutrophil accumulation in the colon tissue of the Fe3-δO4 nanoparticle-treated mice. Fe3-δO4 nanoparticles, which had no influence on gut microbiota and apparent side effects in vivo, were efficacious inhibitors of C. difficile spore germination by attacking its surface and might become clinically feasible for prophylaxis and therapy.Wei-Ting LeeYa-Na WuYi-Hsuan ChenShang-Rung WuTsai-Miao ShihTsung-Ju LiLi-Xing YangChen-Sheng YehPei-Jane TsaiDar-Bin ShiehNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei-Ting Lee Ya-Na Wu Yi-Hsuan Chen Shang-Rung Wu Tsai-Miao Shih Tsung-Ju Li Li-Xing Yang Chen-Sheng Yeh Pei-Jane Tsai Dar-Bin Shieh Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore Germination and Attenuated Local and Systemic Inflammation
description	Abstract Clinical management of Clostridium difficile infection is still far from satisfactory as bacterial spores are resistant to many chemical agents and physical treatments. Certain types of nanoparticles have been demonstrated to exhibit anti-microbial efficacy even in multi-drug resistance bacteria. However, most of these studies failed to show biocompatibility to the mammalian host cells and no study has revealed in vivo efficacy in C. difficile infection animal models. The spores treated with 500 µg/mL Fe3-δO4 nanoparticles for 20 minutes, 64% of the spores were inhibited from transforming into vegetative cells, which was close to the results of the sodium hypochlorite-treated positive control. By cryo-electron micro-tomography, we demonstrated that Fe3-δO4 nanoparticles bind on spore surfaces and reduce the dipicolinic acid (DPA) released by the spores. In a C. difficile infection animal model, the inflammatory level triple decreased in mice with colonic C. difficile spores treated with Fe3-δO4 nanoparticles. Histopathological analysis showed a decreased intense neutrophil accumulation in the colon tissue of the Fe3-δO4 nanoparticle-treated mice. Fe3-δO4 nanoparticles, which had no influence on gut microbiota and apparent side effects in vivo, were efficacious inhibitors of C. difficile spore germination by attacking its surface and might become clinically feasible for prophylaxis and therapy.
format	article
author	Wei-Ting Lee Ya-Na Wu Yi-Hsuan Chen Shang-Rung Wu Tsai-Miao Shih Tsung-Ju Li Li-Xing Yang Chen-Sheng Yeh Pei-Jane Tsai Dar-Bin Shieh
author_facet	Wei-Ting Lee Ya-Na Wu Yi-Hsuan Chen Shang-Rung Wu Tsai-Miao Shih Tsung-Ju Li Li-Xing Yang Chen-Sheng Yeh Pei-Jane Tsai Dar-Bin Shieh
author_sort	Wei-Ting Lee
title	Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore Germination and Attenuated Local and Systemic Inflammation
title_short	Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore Germination and Attenuated Local and Systemic Inflammation
title_full	Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore Germination and Attenuated Local and Systemic Inflammation
title_fullStr	Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore Germination and Attenuated Local and Systemic Inflammation
title_full_unstemmed	Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore Germination and Attenuated Local and Systemic Inflammation
title_sort	octahedron iron oxide nanocrystals prohibited clostridium difficile spore germination and attenuated local and systemic inflammation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a00e268fa83d43cbb45283c6def3f567
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Octahedron Iron Oxide Nanocrystals Prohibited Clostridium difficile Spore Germination and Attenuated Local and Systemic Inflammation

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