Liraglutide suppresses obesity and promotes browning of white fat via miR-27b and

Objective To investigate the effect of liraglutide on the browning of white fat and the suppression of obesity via regulating microRNA (miR)-27b in vivo and in vitro . Methods Sprague-Dawley rats were fed a high-fat (HF) diet and 3T3-L1 pre-adipocytes were differentiated into mature white adipocytes...

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Autores principales:	Xing Wang, Shuchun Chen, Dan Lv, Zelin Li, Luping Ren, Haijiao Zhu, Xing Xie, Yang Liu
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a00f4dfd42934772bad196c03aefeb9f
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spelling	oai:doaj.org-article:a00f4dfd42934772bad196c03aefeb9f2021-11-15T00:03:25ZLiraglutide suppresses obesity and promotes browning of white fat via miR-27b and 1473-230010.1177/03000605211055059https://doaj.org/article/a00f4dfd42934772bad196c03aefeb9f2021-11-01T00:00:00Zhttps://doi.org/10.1177/03000605211055059https://doaj.org/toc/1473-2300Objective To investigate the effect of liraglutide on the browning of white fat and the suppression of obesity via regulating microRNA (miR)-27b in vivo and in vitro . Methods Sprague-Dawley rats were fed a high-fat (HF) diet and 3T3-L1 pre-adipocytes were differentiated into mature white adipocytes. Rats and mature adipocytes were then treated with different doses of liraglutide. The mRNA and protein levels of browning-associated proteins, including uncoupling protein 1 (UCP1), PR domain containing 16 (PRDM16), CCAAT enhancer binding protein β (CEBPβ), cell death-inducing DFFA-like effector A (CIDEA) and peroxisome proliferator-activated receptor-γ-coactivator 1α (PGC-1α), were detected using quantitative real-time polymerase chain reaction and Western blotting. Results Liraglutide decreased body weight and reduced the levels of blood glucose, triglyceride and low-density lipoprotein cholesterol in HF diet-fed rats. Liraglutide increased the levels of UCP1, PRDM16, CEBPβ, CIDEA and PGC-1α in vivo and vitro . The levels of miR-27b were upregulated in HF diet-fed rats, whereas liraglutide reduced the levels of miR-27b. In vitro , overexpression of miR-27b decreased the mRNA and protein levels of UCP1, PRDM16, CEBPβ, CIDEA and PGC-1α. Transfection with the miR-27b mimics attenuated the effect of liraglutide on the browning of white adipocytes. Conclusion Liraglutide induced browning of white adipose through regulation of miR-27b.Xing WangShuchun ChenDan LvZelin LiLuping RenHaijiao ZhuXing XieYang LiuSAGE PublishingarticleMedicine (General)R5-920ENJournal of International Medical Research, Vol 49 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Xing Wang Shuchun Chen Dan Lv Zelin Li Luping Ren Haijiao Zhu Xing Xie Yang Liu Liraglutide suppresses obesity and promotes browning of white fat via miR-27b and
description	Objective To investigate the effect of liraglutide on the browning of white fat and the suppression of obesity via regulating microRNA (miR)-27b in vivo and in vitro . Methods Sprague-Dawley rats were fed a high-fat (HF) diet and 3T3-L1 pre-adipocytes were differentiated into mature white adipocytes. Rats and mature adipocytes were then treated with different doses of liraglutide. The mRNA and protein levels of browning-associated proteins, including uncoupling protein 1 (UCP1), PR domain containing 16 (PRDM16), CCAAT enhancer binding protein β (CEBPβ), cell death-inducing DFFA-like effector A (CIDEA) and peroxisome proliferator-activated receptor-γ-coactivator 1α (PGC-1α), were detected using quantitative real-time polymerase chain reaction and Western blotting. Results Liraglutide decreased body weight and reduced the levels of blood glucose, triglyceride and low-density lipoprotein cholesterol in HF diet-fed rats. Liraglutide increased the levels of UCP1, PRDM16, CEBPβ, CIDEA and PGC-1α in vivo and vitro . The levels of miR-27b were upregulated in HF diet-fed rats, whereas liraglutide reduced the levels of miR-27b. In vitro , overexpression of miR-27b decreased the mRNA and protein levels of UCP1, PRDM16, CEBPβ, CIDEA and PGC-1α. Transfection with the miR-27b mimics attenuated the effect of liraglutide on the browning of white adipocytes. Conclusion Liraglutide induced browning of white adipose through regulation of miR-27b.
format	article
author	Xing Wang Shuchun Chen Dan Lv Zelin Li Luping Ren Haijiao Zhu Xing Xie Yang Liu
author_facet	Xing Wang Shuchun Chen Dan Lv Zelin Li Luping Ren Haijiao Zhu Xing Xie Yang Liu
author_sort	Xing Wang
title	Liraglutide suppresses obesity and promotes browning of white fat via miR-27b and
title_short	Liraglutide suppresses obesity and promotes browning of white fat via miR-27b and
title_full	Liraglutide suppresses obesity and promotes browning of white fat via miR-27b and
title_fullStr	Liraglutide suppresses obesity and promotes browning of white fat via miR-27b and
title_full_unstemmed	Liraglutide suppresses obesity and promotes browning of white fat via miR-27b and
title_sort	liraglutide suppresses obesity and promotes browning of white fat via mir-27b and
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/a00f4dfd42934772bad196c03aefeb9f
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Liraglutide suppresses obesity and promotes browning of white fat via miR-27b and

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