Analyses of protein corona on bare and silica-coated gold nanorods against four mammalian cells

Minakshi Das,1 Dong Kee Yi,2 Seong Soo A An1 1Department of Bionanotechnology, Gachon University, Seongnam, Republic of Korea; 2Department of Chemistry, Myongji University, Yongin, Republic of Korea Abstract: The purpose of this study was to investigate the mechanisms responsible for the toxic ef...

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Autores principales:	Das M, Yi DK, An SSA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a011ef4064ec4bd3a0a137f56a84d285
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spelling	oai:doaj.org-article:a011ef4064ec4bd3a0a137f56a84d2852021-12-02T00:31:21ZAnalyses of protein corona on bare and silica-coated gold nanorods against four mammalian cells1178-2013https://doaj.org/article/a011ef4064ec4bd3a0a137f56a84d2852015-02-01T00:00:00Zhttp://www.dovepress.com/analyses-of-protein-corona-on-bare-and-silica-coated-gold-nanorods-aga-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Minakshi Das,1 Dong Kee Yi,2 Seong Soo A An1 1Department of Bionanotechnology, Gachon University, Seongnam, Republic of Korea; 2Department of Chemistry, Myongji University, Yongin, Republic of Korea Abstract: The purpose of this study was to investigate the mechanisms responsible for the toxic effects of gold nanorods (AuNRs). Here, a comprehensive study was performed by examining the effects of bare (uncoated) AuNRs and AuNRs functionalized with silica (SiO2-AuNRs) against various mammalian cell lines, including cervical cancer cells, fibroblast cells, human umbilical vein endothelial cells, and neuroblastoma cells. The interactions between AuNRs and mammalian cells were investigated with cell viability and mortality assays. Dihydrorhodamine-123 assay was carried out for evaluating reactive oxygen species (ROS) generation, along with mass spectroscopy analysis for determining the composition of the protein corona. Our results suggest that even the lowest concentrations of AuNRs (0.7 µg/mL) induced ROS production leading to cell mortality. On the other hand, cellular viability and ROS production were maintained even at a higher concentration of SiO2-coated AuNRs (12 µg/mL). The increased production of ROS by AuNRs seemed to cause the toxicity observed in all four mammalian cell types. The protein corona on the bare AuNRs did not appear to reduce ROS generation; however, different compositions of the protein corona on bare and SiO2-coated AuNRs may affect cellular behavior differently. Therefore, it was determined that SiO2-coated AuNRs would be more advantageous than bare AuNRs for cellular applications. Keywords: gold nanorods, silica coating, oxidative stress, mammalian cells, cell toxicity, protein coronaDas MYi DKAn SSADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1521-1545 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Das M Yi DK An SSA Analyses of protein corona on bare and silica-coated gold nanorods against four mammalian cells
description	Minakshi Das,1 Dong Kee Yi,2 Seong Soo A An1 1Department of Bionanotechnology, Gachon University, Seongnam, Republic of Korea; 2Department of Chemistry, Myongji University, Yongin, Republic of Korea Abstract: The purpose of this study was to investigate the mechanisms responsible for the toxic effects of gold nanorods (AuNRs). Here, a comprehensive study was performed by examining the effects of bare (uncoated) AuNRs and AuNRs functionalized with silica (SiO2-AuNRs) against various mammalian cell lines, including cervical cancer cells, fibroblast cells, human umbilical vein endothelial cells, and neuroblastoma cells. The interactions between AuNRs and mammalian cells were investigated with cell viability and mortality assays. Dihydrorhodamine-123 assay was carried out for evaluating reactive oxygen species (ROS) generation, along with mass spectroscopy analysis for determining the composition of the protein corona. Our results suggest that even the lowest concentrations of AuNRs (0.7 µg/mL) induced ROS production leading to cell mortality. On the other hand, cellular viability and ROS production were maintained even at a higher concentration of SiO2-coated AuNRs (12 µg/mL). The increased production of ROS by AuNRs seemed to cause the toxicity observed in all four mammalian cell types. The protein corona on the bare AuNRs did not appear to reduce ROS generation; however, different compositions of the protein corona on bare and SiO2-coated AuNRs may affect cellular behavior differently. Therefore, it was determined that SiO2-coated AuNRs would be more advantageous than bare AuNRs for cellular applications. Keywords: gold nanorods, silica coating, oxidative stress, mammalian cells, cell toxicity, protein corona
format	article
author	Das M Yi DK An SSA
author_facet	Das M Yi DK An SSA
author_sort	Das M
title	Analyses of protein corona on bare and silica-coated gold nanorods against four mammalian cells
title_short	Analyses of protein corona on bare and silica-coated gold nanorods against four mammalian cells
title_full	Analyses of protein corona on bare and silica-coated gold nanorods against four mammalian cells
title_fullStr	Analyses of protein corona on bare and silica-coated gold nanorods against four mammalian cells
title_full_unstemmed	Analyses of protein corona on bare and silica-coated gold nanorods against four mammalian cells
title_sort	analyses of protein corona on bare and silica-coated gold nanorods against four mammalian cells
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/a011ef4064ec4bd3a0a137f56a84d285
work_keys_str_mv	AT dasm analysesofproteincoronaonbareandsilicacoatedgoldnanorodsagainstfourmammaliannbspcells AT yidk analysesofproteincoronaonbareandsilicacoatedgoldnanorodsagainstfourmammaliannbspcells AT anssa analysesofproteincoronaonbareandsilicacoatedgoldnanorodsagainstfourmammaliannbspcells
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Analyses of protein corona on bare and silica-coated gold nanorods against four mammalian&nbsp;cells

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Analyses of protein corona on bare and silica-coated gold nanorods against four mammalian cells