Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising and reliable tool for arthropod identification, including the identification of alcohol-preserved ticks based on extracted leg protein spectra. In this study, the legs of 361...

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Autores principales:	Ly Na Huynh, Adama Zan Diarra, Quang Luan Pham, Nhiem Le-Viet, Jean-Michel Berenger, Van Hoang Ho, Xuan Quang Nguyen, Philippe Parola
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/a02219298f5c4bbb8f76fe224385a5e3
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spelling	oai:doaj.org-article:a02219298f5c4bbb8f76fe224385a5e32021-12-02T20:24:01ZMorphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.1935-27271935-273510.1371/journal.pntd.0009813https://doaj.org/article/a02219298f5c4bbb8f76fe224385a5e32021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009813https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising and reliable tool for arthropod identification, including the identification of alcohol-preserved ticks based on extracted leg protein spectra. In this study, the legs of 361 ticks collected in Vietnam, including 251 Rhiphicephalus sanguineus s.l, 99 Rhipicephalus (Boophilus) microplus, two Amblyomma varanensis, seven Dermacentor auratus, one Dermacentor compactus and one Amblyomma sp. were submitted for MALDI-TOF MS analyses. Spectral analysis showed intra-species reproducibility and inter-species specificity and the spectra of 329 (91%) specimens were of excellent quality. The blind test of 310 spectra remaining after updating the database with 19 spectra revealed that all were correctly identified with log score values (LSV) ranging from 1.7 to 2.396 with a mean of 1.982 ± 0.142 and a median of 1.971. The DNA of several microorganisms including Anaplasma platys, Anaplasma phagocytophilum, Anaplasma marginale, Ehrlichia rustica, Babesia vogeli, Theileria sinensis, and Theileria orientalis were detected in 25 ticks. Co-infection by A. phagocytophilum and T. sinensis was found in one Rh. (B) microplus.Ly Na HuynhAdama Zan DiarraQuang Luan PhamNhiem Le-VietJean-Michel BerengerVan Hoang HoXuan Quang NguyenPhilippe ParolaPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009813 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ly Na Huynh Adama Zan Diarra Quang Luan Pham Nhiem Le-Viet Jean-Michel Berenger Van Hoang Ho Xuan Quang Nguyen Philippe Parola Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.
description	Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising and reliable tool for arthropod identification, including the identification of alcohol-preserved ticks based on extracted leg protein spectra. In this study, the legs of 361 ticks collected in Vietnam, including 251 Rhiphicephalus sanguineus s.l, 99 Rhipicephalus (Boophilus) microplus, two Amblyomma varanensis, seven Dermacentor auratus, one Dermacentor compactus and one Amblyomma sp. were submitted for MALDI-TOF MS analyses. Spectral analysis showed intra-species reproducibility and inter-species specificity and the spectra of 329 (91%) specimens were of excellent quality. The blind test of 310 spectra remaining after updating the database with 19 spectra revealed that all were correctly identified with log score values (LSV) ranging from 1.7 to 2.396 with a mean of 1.982 ± 0.142 and a median of 1.971. The DNA of several microorganisms including Anaplasma platys, Anaplasma phagocytophilum, Anaplasma marginale, Ehrlichia rustica, Babesia vogeli, Theileria sinensis, and Theileria orientalis were detected in 25 ticks. Co-infection by A. phagocytophilum and T. sinensis was found in one Rh. (B) microplus.
format	article
author	Ly Na Huynh Adama Zan Diarra Quang Luan Pham Nhiem Le-Viet Jean-Michel Berenger Van Hoang Ho Xuan Quang Nguyen Philippe Parola
author_facet	Ly Na Huynh Adama Zan Diarra Quang Luan Pham Nhiem Le-Viet Jean-Michel Berenger Van Hoang Ho Xuan Quang Nguyen Philippe Parola
author_sort	Ly Na Huynh
title	Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.
title_short	Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.
title_full	Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.
title_fullStr	Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.
title_full_unstemmed	Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.
title_sort	morphological, molecular and maldi-tof ms identification of ticks and tick-associated pathogens in vietnam.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a02219298f5c4bbb8f76fe224385a5e3
work_keys_str_mv	AT lynahuynh morphologicalmolecularandmalditofmsidentificationofticksandtickassociatedpathogensinvietnam AT adamazandiarra morphologicalmolecularandmalditofmsidentificationofticksandtickassociatedpathogensinvietnam AT quangluanpham morphologicalmolecularandmalditofmsidentificationofticksandtickassociatedpathogensinvietnam AT nhiemleviet morphologicalmolecularandmalditofmsidentificationofticksandtickassociatedpathogensinvietnam AT jeanmichelberenger morphologicalmolecularandmalditofmsidentificationofticksandtickassociatedpathogensinvietnam AT vanhoangho morphologicalmolecularandmalditofmsidentificationofticksandtickassociatedpathogensinvietnam AT xuanquangnguyen morphologicalmolecularandmalditofmsidentificationofticksandtickassociatedpathogensinvietnam AT philippeparola morphologicalmolecularandmalditofmsidentificationofticksandtickassociatedpathogensinvietnam
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Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.

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