Lipase degradation of plasticized polyvinyl chloride endotracheal tube surfaces to create nanoscale features

Mary C Machado,1 Thomas J Webster2 1Center for Biomedical Engineering, Division of Engineering Brown University, Providence, RI, 2Department of Chemical Engineering, Northeastern University, Boston, MA, USA Abstract: Polyvinyl chloride (PVC) endotracheal tubes (ETTs) nanoetched with a fungal lipas...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Machado MC, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	bacteria nanoscale features roughness endotracheal tubes VAP Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a0227f1c70e34928b4b0afd3a8bf6e65
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a0227f1c70e34928b4b0afd3a8bf6e65
record_format	dspace
spelling	oai:doaj.org-article:a0227f1c70e34928b4b0afd3a8bf6e652021-12-02T07:28:03ZLipase degradation of plasticized polyvinyl chloride endotracheal tube surfaces to create nanoscale features1178-2013https://doaj.org/article/a0227f1c70e34928b4b0afd3a8bf6e652017-03-01T00:00:00Zhttps://www.dovepress.com/lipase-degradation-of-plasticized-polyvinyl-chloride-endotracheal-tube-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mary C Machado,1 Thomas J Webster2 1Center for Biomedical Engineering, Division of Engineering Brown University, Providence, RI, 2Department of Chemical Engineering, Northeastern University, Boston, MA, USA Abstract: Polyvinyl chloride (PVC) endotracheal tubes (ETTs) nanoetched with a fungal lipase have been shown to reduce bacterial growth and biofilm formation and could be an inexpensive solution to the complex problem of ventilator-associated pneumonia (VAP). Although bacterial growth and colonization on these nanoetched materials have been well characterized, little is known about the mechanism by which the fungal lipase degrades the PVC and, thus, alters its properties to minimize bacteria functions. This study used X-ray photoelectron spectroscopy (XPS) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) to better describe the surface chemistry of both unetched and lipase nanoetched PVC ETT. ATR-FTIR analysis of the unetched and treated surfaces showed a similar presence of a plasticizer. This was confirmed by XPS analysis, which showed an increase of carbon and the presence of oxygen on both unetched and nanoetched surfaces. A quantitative comparison of the FTIR spectra revealed significant correlations (Pearson’s correlation, R=0.997 [R2=0.994, P<0.001]) between the unetched and nanomodified PVC ETT spectra, demonstrating similar surface chemistry. This analysis showed no shifting or widening of the bands in the spectra and no significant changes in the intensity of the infrared peaks due to the degradation of the plasticizer by the fungal lipase. In contrast, results from this study did demonstrate significantly increased nanoscale surface features on the lipase etched compared to non-etched PVC ETTs. This led to a change in surface energetics, which altered ion adsorption to the ETTs. Thus, these results showed that PVC surfaces nanoetched with a 0.1% lipase solution for 48 hours have no significant change on surface chemistry but do significantly increase nanoscale surface roughness and alters ion adsorption, which suggests that the unique properties of these materials, including their previously reported ability to decrease bacterial adhesion and growth, are due to the changes in the degree of the nanoscale roughness, not changes in their surface chemistry. Keywords: nanorough, lipase, hydrolysis, spectroscopy, polymerMachado MCWebster TJDove Medical Pressarticlebacteriananoscale featuresroughnessendotracheal tubesVAPMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2109-2115 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteria nanoscale features roughness endotracheal tubes VAP Medicine (General) R5-920
spellingShingle	bacteria nanoscale features roughness endotracheal tubes VAP Medicine (General) R5-920 Machado MC Webster TJ Lipase degradation of plasticized polyvinyl chloride endotracheal tube surfaces to create nanoscale features
description	Mary C Machado,1 Thomas J Webster2 1Center for Biomedical Engineering, Division of Engineering Brown University, Providence, RI, 2Department of Chemical Engineering, Northeastern University, Boston, MA, USA Abstract: Polyvinyl chloride (PVC) endotracheal tubes (ETTs) nanoetched with a fungal lipase have been shown to reduce bacterial growth and biofilm formation and could be an inexpensive solution to the complex problem of ventilator-associated pneumonia (VAP). Although bacterial growth and colonization on these nanoetched materials have been well characterized, little is known about the mechanism by which the fungal lipase degrades the PVC and, thus, alters its properties to minimize bacteria functions. This study used X-ray photoelectron spectroscopy (XPS) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) to better describe the surface chemistry of both unetched and lipase nanoetched PVC ETT. ATR-FTIR analysis of the unetched and treated surfaces showed a similar presence of a plasticizer. This was confirmed by XPS analysis, which showed an increase of carbon and the presence of oxygen on both unetched and nanoetched surfaces. A quantitative comparison of the FTIR spectra revealed significant correlations (Pearson’s correlation, R=0.997 [R2=0.994, P<0.001]) between the unetched and nanomodified PVC ETT spectra, demonstrating similar surface chemistry. This analysis showed no shifting or widening of the bands in the spectra and no significant changes in the intensity of the infrared peaks due to the degradation of the plasticizer by the fungal lipase. In contrast, results from this study did demonstrate significantly increased nanoscale surface features on the lipase etched compared to non-etched PVC ETTs. This led to a change in surface energetics, which altered ion adsorption to the ETTs. Thus, these results showed that PVC surfaces nanoetched with a 0.1% lipase solution for 48 hours have no significant change on surface chemistry but do significantly increase nanoscale surface roughness and alters ion adsorption, which suggests that the unique properties of these materials, including their previously reported ability to decrease bacterial adhesion and growth, are due to the changes in the degree of the nanoscale roughness, not changes in their surface chemistry. Keywords: nanorough, lipase, hydrolysis, spectroscopy, polymer
format	article
author	Machado MC Webster TJ
author_facet	Machado MC Webster TJ
author_sort	Machado MC
title	Lipase degradation of plasticized polyvinyl chloride endotracheal tube surfaces to create nanoscale features
title_short	Lipase degradation of plasticized polyvinyl chloride endotracheal tube surfaces to create nanoscale features
title_full	Lipase degradation of plasticized polyvinyl chloride endotracheal tube surfaces to create nanoscale features
title_fullStr	Lipase degradation of plasticized polyvinyl chloride endotracheal tube surfaces to create nanoscale features
title_full_unstemmed	Lipase degradation of plasticized polyvinyl chloride endotracheal tube surfaces to create nanoscale features
title_sort	lipase degradation of plasticized polyvinyl chloride endotracheal tube surfaces to create nanoscale features
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/a0227f1c70e34928b4b0afd3a8bf6e65
work_keys_str_mv	AT machadomc lipasedegradationofplasticizedpolyvinylchlorideendotrachealtubesurfacestocreatenanoscalefeatures AT webstertj lipasedegradationofplasticizedpolyvinylchlorideendotrachealtubesurfacestocreatenanoscalefeatures
_version_	1718399417157943296

Lipase degradation of plasticized polyvinyl chloride endotracheal tube surfaces to create nanoscale features

Ejemplares similares