Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)

Abstract In the present study, two elongases, Elovl4 and Elovl5, were functionally characterized and their transcriptional regulation in response to n-3 LC-PUFA administration were investigated in vivo and in vitro. We previously described the molecular characterization of croaker elovl5. Here, we r...

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Autores principales:	Songlin Li, Óscar Monroig, Tianjiao Wang, Yuhui Yuan, Juan Carlos Navarro, Francisco Hontoria, Kai Liao, Douglas R. Tocher, Kangsen Mai, Wei Xu, Qinghui Ai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a0279deb36744645a8117f86db8ecf97
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spelling	oai:doaj.org-article:a0279deb36744645a8117f86db8ecf972021-12-02T15:06:19ZFunctional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)10.1038/s41598-017-02646-82045-2322https://doaj.org/article/a0279deb36744645a8117f86db8ecf972017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02646-8https://doaj.org/toc/2045-2322Abstract In the present study, two elongases, Elovl4 and Elovl5, were functionally characterized and their transcriptional regulation in response to n-3 LC-PUFA administration were investigated in vivo and in vitro. We previously described the molecular characterization of croaker elovl5. Here, we report the full-length cDNA sequence of croaker elovl4, which contained 1794 bp (excluding the polyA tail), including 909 bp of coding region that encoded a polypeptide of 302 amino acids possessing all the characteristic features of Elovl proteins. Functional studies showed that croaker Elovl5, displayed high elongation activity towards C18 and C20 PUFA, with only low activity towards C22 PUFA. In contrast, croaker Elovl4 could effectively convert both C20 and C22 PUFA to longer polyenoic products up to C34. n-3 LC-PUFA suppressed transcription of the two elongase genes, as well as srebp-1 and lxrα, major regulators of hepatic lipid metabolism. The results of dual-luciferase reporter assays and in vitro studies both indicated that the transcriptions of elovl5 and elovl4 elongases could be regulated by Lxrα. Moreover, Lxrα could mediate the transcription of elovl4 directly or indirectly through regulating the transcription of srebp-1. The above findings contribute further insight and understanding of the mechanisms regulating LC-PUFA biosynthesis in marine fish species.Songlin LiÓscar MonroigTianjiao WangYuhui YuanJuan Carlos NavarroFrancisco HontoriaKai LiaoDouglas R. TocherKangsen MaiWei XuQinghui AiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Songlin Li Óscar Monroig Tianjiao Wang Yuhui Yuan Juan Carlos Navarro Francisco Hontoria Kai Liao Douglas R. Tocher Kangsen Mai Wei Xu Qinghui Ai Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)
description	Abstract In the present study, two elongases, Elovl4 and Elovl5, were functionally characterized and their transcriptional regulation in response to n-3 LC-PUFA administration were investigated in vivo and in vitro. We previously described the molecular characterization of croaker elovl5. Here, we report the full-length cDNA sequence of croaker elovl4, which contained 1794 bp (excluding the polyA tail), including 909 bp of coding region that encoded a polypeptide of 302 amino acids possessing all the characteristic features of Elovl proteins. Functional studies showed that croaker Elovl5, displayed high elongation activity towards C18 and C20 PUFA, with only low activity towards C22 PUFA. In contrast, croaker Elovl4 could effectively convert both C20 and C22 PUFA to longer polyenoic products up to C34. n-3 LC-PUFA suppressed transcription of the two elongase genes, as well as srebp-1 and lxrα, major regulators of hepatic lipid metabolism. The results of dual-luciferase reporter assays and in vitro studies both indicated that the transcriptions of elovl5 and elovl4 elongases could be regulated by Lxrα. Moreover, Lxrα could mediate the transcription of elovl4 directly or indirectly through regulating the transcription of srebp-1. The above findings contribute further insight and understanding of the mechanisms regulating LC-PUFA biosynthesis in marine fish species.
format	article
author	Songlin Li Óscar Monroig Tianjiao Wang Yuhui Yuan Juan Carlos Navarro Francisco Hontoria Kai Liao Douglas R. Tocher Kangsen Mai Wei Xu Qinghui Ai
author_facet	Songlin Li Óscar Monroig Tianjiao Wang Yuhui Yuan Juan Carlos Navarro Francisco Hontoria Kai Liao Douglas R. Tocher Kangsen Mai Wei Xu Qinghui Ai
author_sort	Songlin Li
title	Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)
title_short	Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)
title_full	Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)
title_fullStr	Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)
title_full_unstemmed	Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)
title_sort	functional characterization and differential nutritional regulation of putative elovl5 and elovl4 elongases in large yellow croaker (larimichthys crocea)
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a0279deb36744645a8117f86db8ecf97
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Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)

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