Immunoaffinity purification of endogenous proteins from S. cerevisiae for post-translational modification and protein interaction analysis
Summary: Protein regulation by post-translational modifications and protein-protein interactions is critical to controlling molecular pathways. Here, we describe an immunoaffinity purification approach in Saccharomyces cerevisiae. The protocol uses an endogenously-expressed epitope-tagged protein an...
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| Main Authors: | , , |
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| Format: | article |
| Language: | EN |
| Published: |
Elsevier
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/a02a8b8b117e482487c9c01e5a50d630 |
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