Immunoaffinity purification of endogenous proteins from S. cerevisiae for post-translational modification and protein interaction analysis

Summary: Protein regulation by post-translational modifications and protein-protein interactions is critical to controlling molecular pathways. Here, we describe an immunoaffinity purification approach in Saccharomyces cerevisiae. The protocol uses an endogenously-expressed epitope-tagged protein an...

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Auteurs principaux: Deepika Jaiswal, Rashi Turniansky, Erin M. Green
Format: article
Langue:EN
Publié: Elsevier 2021
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Accès en ligne:https://doaj.org/article/a02a8b8b117e482487c9c01e5a50d630
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