Internalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys

Shang Ziyad Abdulqadir, Falah Mohammad AzizBiology Department, Salahaddin University-Erbil, Erbil, Kurdistan Region, IraqPurpose: Since nanoparticles (NPs) are beginning to be introduced in medicine and industry, it is mendatory to evaluate their biological side-effects, among other things. The pres...

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Autores principales:	Abdulqadir SZ, Aziz FM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Nickel nanoparticles internalization ultrastructure proximal convoluted tubules podocytes mesangial cells Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a02b7a294d4445529f066f44310e9d94
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spelling	oai:doaj.org-article:a02b7a294d4445529f066f44310e9d942021-12-02T05:40:29ZInternalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys1178-2013https://doaj.org/article/a02b7a294d4445529f066f44310e9d942019-05-01T00:00:00Zhttps://www.dovepress.com/internalization-and-effects-on-cellular-ultrastructure-of-nickel-nanop-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Shang Ziyad Abdulqadir, Falah Mohammad AzizBiology Department, Salahaddin University-Erbil, Erbil, Kurdistan Region, IraqPurpose: Since nanoparticles (NPs) are beginning to be introduced in medicine and industry, it is mendatory to evaluate their biological side-effects, among other things. The present study aimed to investigate the pathways by which nickel nanoparticles (NiNPs) enter nephrons and to evaluate their localization and effects on cellular ultrastructure.Methods: Rats were injected intraperitoneally with 20 nm NiNPs (20 mg/Kg/b.w./day) for 28 consecutive days. Transmission electron microscope technique was used to detect localization of NiNPs and their effects on cellular ultrastructure in rat kidneys. Additionally, measurements of certain biochemical parameters such as creatinine, urea, uric acid and phosphorus for investigating renal function following NiNPs treatment were taken.Results: The presence of NiNPs in the nephrons in treated rats was confirmed by transmission electron microscopy. NiNPs entered the renal tubules cells via various pathways. The results indicated that NiNPs administration induced ultrastructural changes in the proximal cells of renal tubules and certain glomerular cells (podocytes and mesangial cells). Additionally, NiNPs were found to be localized in the mitochondria, which led to a significant decrease in their density and morphology. Furthermore, cell death was induced in the glomerular cells as found with a Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) assay and through detection of p35 using immunohistochemical staining.Conclusion: Herein, NiNPs were found to induce various cellular ultrastructural changes in the kidneys of rats. NiNPs used diverse pathways to internalize into the cytoplasm of the proximal convoluted tubules (PT) cells across the basement membrane, and also through the plasma membrane of two adjacent PT cells. NiNPs internalization, accumulation and their alterations of the cellular ultrastructure affected rat renal function.Keywords: Nickel nanoparticles, internalization, ultrastructure, proximal convoluted tubules, podocytes, mesangial cellsAbdulqadir SZAziz FMDove Medical PressarticleNickel nanoparticlesinternalizationultrastructureproximal convoluted tubulespodocytesmesangial cellsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3995-4005 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nickel nanoparticles internalization ultrastructure proximal convoluted tubules podocytes mesangial cells Medicine (General) R5-920
spellingShingle	Nickel nanoparticles internalization ultrastructure proximal convoluted tubules podocytes mesangial cells Medicine (General) R5-920 Abdulqadir SZ Aziz FM Internalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys
description	Shang Ziyad Abdulqadir, Falah Mohammad AzizBiology Department, Salahaddin University-Erbil, Erbil, Kurdistan Region, IraqPurpose: Since nanoparticles (NPs) are beginning to be introduced in medicine and industry, it is mendatory to evaluate their biological side-effects, among other things. The present study aimed to investigate the pathways by which nickel nanoparticles (NiNPs) enter nephrons and to evaluate their localization and effects on cellular ultrastructure.Methods: Rats were injected intraperitoneally with 20 nm NiNPs (20 mg/Kg/b.w./day) for 28 consecutive days. Transmission electron microscope technique was used to detect localization of NiNPs and their effects on cellular ultrastructure in rat kidneys. Additionally, measurements of certain biochemical parameters such as creatinine, urea, uric acid and phosphorus for investigating renal function following NiNPs treatment were taken.Results: The presence of NiNPs in the nephrons in treated rats was confirmed by transmission electron microscopy. NiNPs entered the renal tubules cells via various pathways. The results indicated that NiNPs administration induced ultrastructural changes in the proximal cells of renal tubules and certain glomerular cells (podocytes and mesangial cells). Additionally, NiNPs were found to be localized in the mitochondria, which led to a significant decrease in their density and morphology. Furthermore, cell death was induced in the glomerular cells as found with a Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) assay and through detection of p35 using immunohistochemical staining.Conclusion: Herein, NiNPs were found to induce various cellular ultrastructural changes in the kidneys of rats. NiNPs used diverse pathways to internalize into the cytoplasm of the proximal convoluted tubules (PT) cells across the basement membrane, and also through the plasma membrane of two adjacent PT cells. NiNPs internalization, accumulation and their alterations of the cellular ultrastructure affected rat renal function.Keywords: Nickel nanoparticles, internalization, ultrastructure, proximal convoluted tubules, podocytes, mesangial cells
format	article
author	Abdulqadir SZ Aziz FM
author_facet	Abdulqadir SZ Aziz FM
author_sort	Abdulqadir SZ
title	Internalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys
title_short	Internalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys
title_full	Internalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys
title_fullStr	Internalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys
title_full_unstemmed	Internalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys
title_sort	internalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/a02b7a294d4445529f066f44310e9d94
work_keys_str_mv	AT abdulqadirsz internalizationandeffectsoncellularultrastructureofnickelnanoparticlesinratkidneys AT azizfm internalizationandeffectsoncellularultrastructureofnickelnanoparticlesinratkidneys
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Internalization and effects on cellular ultrastructure of nickel nanoparticles in rat kidneys

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