Strategies for the hypothermic preservation of cell sheets of human adipose stem cells.

Cell Sheet (CS) Engineering is a regenerative medicine strategy proposed for the treatment of injured or diseased organs and tissues. In fact, several clinical trials are underway using CS-based methodologies. However, the clinical application of such cell-based methodologies poses several challenge...

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Autores principales:	Sara Freitas-Ribeiro, Andreia Filipa Carvalho, Marina Costa, Mariana Teixeira Cerqueira, Alexandra Pinto Marques, Rui Luís Reis, Rogério Pedro Pirraco
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a0394d786cfd40a78b264c593aa33ab7
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spelling	oai:doaj.org-article:a0394d786cfd40a78b264c593aa33ab72021-12-02T20:11:30ZStrategies for the hypothermic preservation of cell sheets of human adipose stem cells.1932-620310.1371/journal.pone.0222597https://doaj.org/article/a0394d786cfd40a78b264c593aa33ab72019-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0222597https://doaj.org/toc/1932-6203Cell Sheet (CS) Engineering is a regenerative medicine strategy proposed for the treatment of injured or diseased organs and tissues. In fact, several clinical trials are underway using CS-based methodologies. However, the clinical application of such cell-based methodologies poses several challenges related with the preservation of CS structure and function from the fabrication site to the bedside. Pausing cells at hypothermic temperatures has been suggested as a valuable method for short-term cell preservation. In this study, we tested the efficiency of two preservation strategies, one using culture medium supplementation with Rokepie and the other using the preservation solution Hypothermosol, in preserving human adipose stromal/stem cells (hASC) CS-like confluent cultures at 4°C, during 3 and 7 days. Both preservation strategies demonstrated excellent ability to preserve cell function during the first 3 days in hypothermia, as demonstrated by metabolic activity results and assessment of extracellular matrix integrity and differentiation potential. At the end of the 7th day of hypothermic incubation, the decrease in cell metabolic activity was more evident for all conditions. Nonetheless, hASC incubated with Rokepie and Hypothermosol retained a higher metabolic activity and extracellular matrix integrity in comparison with unsupplemented cells. Differentiation results for the later time point showed that supplementation with both Rokepie and Hypothermosol rescued adipogenic differentiation potential but only Rokepie was able to preserve hASC osteogenic potential.Sara Freitas-RibeiroAndreia Filipa CarvalhoMarina CostaMariana Teixeira CerqueiraAlexandra Pinto MarquesRui Luís ReisRogério Pedro PirracoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 14, Iss 10, p e0222597 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sara Freitas-Ribeiro Andreia Filipa Carvalho Marina Costa Mariana Teixeira Cerqueira Alexandra Pinto Marques Rui Luís Reis Rogério Pedro Pirraco Strategies for the hypothermic preservation of cell sheets of human adipose stem cells.
description	Cell Sheet (CS) Engineering is a regenerative medicine strategy proposed for the treatment of injured or diseased organs and tissues. In fact, several clinical trials are underway using CS-based methodologies. However, the clinical application of such cell-based methodologies poses several challenges related with the preservation of CS structure and function from the fabrication site to the bedside. Pausing cells at hypothermic temperatures has been suggested as a valuable method for short-term cell preservation. In this study, we tested the efficiency of two preservation strategies, one using culture medium supplementation with Rokepie and the other using the preservation solution Hypothermosol, in preserving human adipose stromal/stem cells (hASC) CS-like confluent cultures at 4°C, during 3 and 7 days. Both preservation strategies demonstrated excellent ability to preserve cell function during the first 3 days in hypothermia, as demonstrated by metabolic activity results and assessment of extracellular matrix integrity and differentiation potential. At the end of the 7th day of hypothermic incubation, the decrease in cell metabolic activity was more evident for all conditions. Nonetheless, hASC incubated with Rokepie and Hypothermosol retained a higher metabolic activity and extracellular matrix integrity in comparison with unsupplemented cells. Differentiation results for the later time point showed that supplementation with both Rokepie and Hypothermosol rescued adipogenic differentiation potential but only Rokepie was able to preserve hASC osteogenic potential.
format	article
author	Sara Freitas-Ribeiro Andreia Filipa Carvalho Marina Costa Mariana Teixeira Cerqueira Alexandra Pinto Marques Rui Luís Reis Rogério Pedro Pirraco
author_facet	Sara Freitas-Ribeiro Andreia Filipa Carvalho Marina Costa Mariana Teixeira Cerqueira Alexandra Pinto Marques Rui Luís Reis Rogério Pedro Pirraco
author_sort	Sara Freitas-Ribeiro
title	Strategies for the hypothermic preservation of cell sheets of human adipose stem cells.
title_short	Strategies for the hypothermic preservation of cell sheets of human adipose stem cells.
title_full	Strategies for the hypothermic preservation of cell sheets of human adipose stem cells.
title_fullStr	Strategies for the hypothermic preservation of cell sheets of human adipose stem cells.
title_full_unstemmed	Strategies for the hypothermic preservation of cell sheets of human adipose stem cells.
title_sort	strategies for the hypothermic preservation of cell sheets of human adipose stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2019
url	https://doaj.org/article/a0394d786cfd40a78b264c593aa33ab7
work_keys_str_mv	AT sarafreitasribeiro strategiesforthehypothermicpreservationofcellsheetsofhumanadiposestemcells AT andreiafilipacarvalho strategiesforthehypothermicpreservationofcellsheetsofhumanadiposestemcells AT marinacosta strategiesforthehypothermicpreservationofcellsheetsofhumanadiposestemcells AT marianateixeiracerqueira strategiesforthehypothermicpreservationofcellsheetsofhumanadiposestemcells AT alexandrapintomarques strategiesforthehypothermicpreservationofcellsheetsofhumanadiposestemcells AT ruiluisreis strategiesforthehypothermicpreservationofcellsheetsofhumanadiposestemcells AT rogeriopedropirraco strategiesforthehypothermicpreservationofcellsheetsofhumanadiposestemcells
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Strategies for the hypothermic preservation of cell sheets of human adipose stem cells.

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