Specific microbiome profile in Takayasu’s arteritis and giant cell arteritis

Abstract Recent studies have provided evidence of a close link between specific microbiota and inflammatory disorders. While the vessel wall microbiota has been recently described in large vessel vasculitis (LVV) and controls, the blood microbiome in these diseases has not been previously reported (...

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Autores principales:	Anne Claire Desbois, Dragos Ciocan, David Saadoun, Gabriel Perlemuter, Patrice Cacoub
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a03c4a8cd8284e6c8265025d984570cb
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spelling	oai:doaj.org-article:a03c4a8cd8284e6c8265025d984570cb2021-12-02T13:17:49ZSpecific microbiome profile in Takayasu’s arteritis and giant cell arteritis10.1038/s41598-021-84725-52045-2322https://doaj.org/article/a03c4a8cd8284e6c8265025d984570cb2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84725-5https://doaj.org/toc/2045-2322Abstract Recent studies have provided evidence of a close link between specific microbiota and inflammatory disorders. While the vessel wall microbiota has been recently described in large vessel vasculitis (LVV) and controls, the blood microbiome in these diseases has not been previously reported (LVV). We aimed to analyse the blood microbiome profile of LVV patients (Takayasu’s arteritis [TAK], giant cell arteritis [GCA]) and healthy blood donors (HD). We studied the blood samples of 13 patients with TAK (20 samples), 9 patients with GCA (11 samples) and 15 HD patients. We assessed the blood microbiome profile by sequencing the 16S rDNA blood bacterial DNA. We used linear discriminant analysis (LDA) coupled with linear discriminant effect size measurement (LEfSe) to investigate the differences in the blood microbiome profile between TAK and GCA patients. An increase in the levels of Clostridia, Cytophagia and Deltaproteobacteria and a decrease in Bacilli at the class level were found in TAK patients compared with HD patients (LDA > 2, p < 0.05). Active TAK patients had significantly lower levels of Staphylococcus compared with inactive TAK patients. Samples of GCA patients had an increased abundance of Rhodococcus and an unidentified member of the Cytophagaceae family. Microbiota of TAK compared with GCA patients was found to show higher levels of Candidatus Aquiluna and Cloacibacterium (LDA > 2; p < 0.05). Differences highlighted in the blood microbiome were also associated with a shift of bacterial predicted metabolic functions in TAK in comparison with HD. Similar results were also found in patients with active versus inactive TAK. In conclusion, patients with TAK were found to present a specific blood microbiome profile in comparison with healthy donors and GCA subjects. Significant changes in the blood microbiome profiles of TAK patients were associated with specific metabolic functions.Anne Claire DesboisDragos CiocanDavid SaadounGabriel PerlemuterPatrice CacoubNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anne Claire Desbois Dragos Ciocan David Saadoun Gabriel Perlemuter Patrice Cacoub Specific microbiome profile in Takayasu’s arteritis and giant cell arteritis
description	Abstract Recent studies have provided evidence of a close link between specific microbiota and inflammatory disorders. While the vessel wall microbiota has been recently described in large vessel vasculitis (LVV) and controls, the blood microbiome in these diseases has not been previously reported (LVV). We aimed to analyse the blood microbiome profile of LVV patients (Takayasu’s arteritis [TAK], giant cell arteritis [GCA]) and healthy blood donors (HD). We studied the blood samples of 13 patients with TAK (20 samples), 9 patients with GCA (11 samples) and 15 HD patients. We assessed the blood microbiome profile by sequencing the 16S rDNA blood bacterial DNA. We used linear discriminant analysis (LDA) coupled with linear discriminant effect size measurement (LEfSe) to investigate the differences in the blood microbiome profile between TAK and GCA patients. An increase in the levels of Clostridia, Cytophagia and Deltaproteobacteria and a decrease in Bacilli at the class level were found in TAK patients compared with HD patients (LDA > 2, p < 0.05). Active TAK patients had significantly lower levels of Staphylococcus compared with inactive TAK patients. Samples of GCA patients had an increased abundance of Rhodococcus and an unidentified member of the Cytophagaceae family. Microbiota of TAK compared with GCA patients was found to show higher levels of Candidatus Aquiluna and Cloacibacterium (LDA > 2; p < 0.05). Differences highlighted in the blood microbiome were also associated with a shift of bacterial predicted metabolic functions in TAK in comparison with HD. Similar results were also found in patients with active versus inactive TAK. In conclusion, patients with TAK were found to present a specific blood microbiome profile in comparison with healthy donors and GCA subjects. Significant changes in the blood microbiome profiles of TAK patients were associated with specific metabolic functions.
format	article
author	Anne Claire Desbois Dragos Ciocan David Saadoun Gabriel Perlemuter Patrice Cacoub
author_facet	Anne Claire Desbois Dragos Ciocan David Saadoun Gabriel Perlemuter Patrice Cacoub
author_sort	Anne Claire Desbois
title	Specific microbiome profile in Takayasu’s arteritis and giant cell arteritis
title_short	Specific microbiome profile in Takayasu’s arteritis and giant cell arteritis
title_full	Specific microbiome profile in Takayasu’s arteritis and giant cell arteritis
title_fullStr	Specific microbiome profile in Takayasu’s arteritis and giant cell arteritis
title_full_unstemmed	Specific microbiome profile in Takayasu’s arteritis and giant cell arteritis
title_sort	specific microbiome profile in takayasu’s arteritis and giant cell arteritis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a03c4a8cd8284e6c8265025d984570cb
work_keys_str_mv	AT anneclairedesbois specificmicrobiomeprofileintakayasusarteritisandgiantcellarteritis AT dragosciocan specificmicrobiomeprofileintakayasusarteritisandgiantcellarteritis AT davidsaadoun specificmicrobiomeprofileintakayasusarteritisandgiantcellarteritis AT gabrielperlemuter specificmicrobiomeprofileintakayasusarteritisandgiantcellarteritis AT patricecacoub specificmicrobiomeprofileintakayasusarteritisandgiantcellarteritis
_version_	1718393347692822528

Specific microbiome profile in Takayasu’s arteritis and giant cell arteritis

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