PEG-modified gadolinium nanoparticles as contrast agents for in vivo micro-CT

Abstract Vascular research is largely performed in rodents with the goal of developing treatments for human disease. Micro-computed tomography (micro-CT) provides non-destructive three-dimensional imaging that can be used to study the vasculature of rodents. However, to distinguish vasculature from...

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Autores principales:	Charmainne Cruje, P. Joy Dunmore-Buyze, Eric Grolman, David W. Holdsworth, Elizabeth R. Gillies, Maria Drangova
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a03f14e146be4f4791616db288a2626d
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spelling	oai:doaj.org-article:a03f14e146be4f4791616db288a2626d2021-12-02T16:45:47ZPEG-modified gadolinium nanoparticles as contrast agents for in vivo micro-CT10.1038/s41598-021-95716-x2045-2322https://doaj.org/article/a03f14e146be4f4791616db288a2626d2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95716-xhttps://doaj.org/toc/2045-2322Abstract Vascular research is largely performed in rodents with the goal of developing treatments for human disease. Micro-computed tomography (micro-CT) provides non-destructive three-dimensional imaging that can be used to study the vasculature of rodents. However, to distinguish vasculature from other soft tissues, long-circulating contrast agents are required. In this study, we demonstrated that poly(ethylene glycol) (PEG)-coated gadolinium nanoparticles can be used as a vascular contrast agent in micro-CT. The coated particles could be lyophilized and then redispersed in an aqueous solution to achieve 100 mg/mL of gadolinium. After an intravenous injection of the contrast agent into mice, micro-CT scans showed blood pool contrast enhancements of at least 200 HU for 30 min. Imaging and quantitative analysis of gadolinium in tissues showed the presence of contrast agent in clearance organs including the liver and spleen and very low amounts in other organs. In vitro cell culture experiments, subcutaneous injections, and analysis of mouse body weight suggested that the agents exhibited low toxicity. Histological analysis of tissues 5 days after injection of the contrast agent showed cytotoxicity in the spleen, but no abnormalities were observed in the liver, lungs, kidneys, and bladder.Charmainne CrujeP. Joy Dunmore-BuyzeEric GrolmanDavid W. HoldsworthElizabeth R. GilliesMaria DrangovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charmainne Cruje P. Joy Dunmore-Buyze Eric Grolman David W. Holdsworth Elizabeth R. Gillies Maria Drangova PEG-modified gadolinium nanoparticles as contrast agents for in vivo micro-CT
description	Abstract Vascular research is largely performed in rodents with the goal of developing treatments for human disease. Micro-computed tomography (micro-CT) provides non-destructive three-dimensional imaging that can be used to study the vasculature of rodents. However, to distinguish vasculature from other soft tissues, long-circulating contrast agents are required. In this study, we demonstrated that poly(ethylene glycol) (PEG)-coated gadolinium nanoparticles can be used as a vascular contrast agent in micro-CT. The coated particles could be lyophilized and then redispersed in an aqueous solution to achieve 100 mg/mL of gadolinium. After an intravenous injection of the contrast agent into mice, micro-CT scans showed blood pool contrast enhancements of at least 200 HU for 30 min. Imaging and quantitative analysis of gadolinium in tissues showed the presence of contrast agent in clearance organs including the liver and spleen and very low amounts in other organs. In vitro cell culture experiments, subcutaneous injections, and analysis of mouse body weight suggested that the agents exhibited low toxicity. Histological analysis of tissues 5 days after injection of the contrast agent showed cytotoxicity in the spleen, but no abnormalities were observed in the liver, lungs, kidneys, and bladder.
format	article
author	Charmainne Cruje P. Joy Dunmore-Buyze Eric Grolman David W. Holdsworth Elizabeth R. Gillies Maria Drangova
author_facet	Charmainne Cruje P. Joy Dunmore-Buyze Eric Grolman David W. Holdsworth Elizabeth R. Gillies Maria Drangova
author_sort	Charmainne Cruje
title	PEG-modified gadolinium nanoparticles as contrast agents for in vivo micro-CT
title_short	PEG-modified gadolinium nanoparticles as contrast agents for in vivo micro-CT
title_full	PEG-modified gadolinium nanoparticles as contrast agents for in vivo micro-CT
title_fullStr	PEG-modified gadolinium nanoparticles as contrast agents for in vivo micro-CT
title_full_unstemmed	PEG-modified gadolinium nanoparticles as contrast agents for in vivo micro-CT
title_sort	peg-modified gadolinium nanoparticles as contrast agents for in vivo micro-ct
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a03f14e146be4f4791616db288a2626d
work_keys_str_mv	AT charmainnecruje pegmodifiedgadoliniumnanoparticlesascontrastagentsforinvivomicroct AT pjoydunmorebuyze pegmodifiedgadoliniumnanoparticlesascontrastagentsforinvivomicroct AT ericgrolman pegmodifiedgadoliniumnanoparticlesascontrastagentsforinvivomicroct AT davidwholdsworth pegmodifiedgadoliniumnanoparticlesascontrastagentsforinvivomicroct AT elizabethrgillies pegmodifiedgadoliniumnanoparticlesascontrastagentsforinvivomicroct AT mariadrangova pegmodifiedgadoliniumnanoparticlesascontrastagentsforinvivomicroct
_version_	1718383470081736704

PEG-modified gadolinium nanoparticles as contrast agents for in vivo micro-CT

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