Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Murugan Veerapandian,1 Yeong-Tai Seo,2 Hyunkyung Shin,3 Kyusik Yun,1 Min-Ho Lee41Department of Bionanotechnology, Gachon University, Gyeonggi-Do, 2School of Electrical Engineering and Computer Science, Seoul National University, Seoul, 3Department of Mathematics and Information, Gachon University, G...

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Autores principales:	Veerapandian M, Seo YT, Shin H, Yun K, Lee MH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a03f8bfdeac349c6b18cccb1391e4cc6
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spelling	oai:doaj.org-article:a03f8bfdeac349c6b18cccb1391e4cc62021-12-02T02:10:24ZFunctionalized graphene oxide for clinical glucose biosensing in urine and serum samples1176-91141178-2013https://doaj.org/article/a03f8bfdeac349c6b18cccb1391e4cc62012-12-01T00:00:00Zhttp://www.dovepress.com/functionalized-graphene-oxide-for-clinical-glucose-biosensing-in-urine-a11784https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Murugan Veerapandian,1 Yeong-Tai Seo,2 Hyunkyung Shin,3 Kyusik Yun,1 Min-Ho Lee41Department of Bionanotechnology, Gachon University, Gyeonggi-Do, 2School of Electrical Engineering and Computer Science, Seoul National University, Seoul, 3Department of Mathematics and Information, Gachon University, Gyeonggi-Do, 4Korea Electronics Technology Institute, Medical IT Technology, Gyeonggi-Do, Republic of KoreaAbstract: A novel clinical glucose biosensor fabricated using functionalized metalloid-polymer (silver-silica coated with polyethylene glycol) hybrid nanoparticles on the surface of a graphene oxide nanosheet is reported. The cyclic voltammetric response of glucose oxidase modification on the surface of a functionalized graphene oxide electrode showed a surface-confined reaction and an effective redox potential near zero volts, with a wide linearity of 0.1&ndash;20 mM and a sensitivity of 7.66 &micro;A mM-1 cm-2. The functionalized graphene oxide electrode showed a better electrocatalytic response toward oxidation of H2O2 and reduction of oxygen. The practical applicability of the functionalized graphene oxide electrode was demonstrated by measuring the peak current against multiple urine and serum samples from diabetic patients. This new hybrid nanoarchitecture combining a three-dimensional metalloid-polymer hybrid and two-dimensional graphene oxide provided a thin solid laminate on the electrode surface. The easy fabrication process and retention of bioactive immobilized enzymes on the functionalized graphene oxide electrode could potentially be extended to detection of other biomolecules, and have broad applications in electrochemical biosensing.Keywords: clinical diagnostics, glucose biosensor, metalloid-polymer nanoparticles, glucose oxidase, cyclic voltammetryVeerapandian MSeo YTShin HYun KLee MHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 6123-6136 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Veerapandian M Seo YT Shin H Yun K Lee MH Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples
description	Murugan Veerapandian,1 Yeong-Tai Seo,2 Hyunkyung Shin,3 Kyusik Yun,1 Min-Ho Lee41Department of Bionanotechnology, Gachon University, Gyeonggi-Do, 2School of Electrical Engineering and Computer Science, Seoul National University, Seoul, 3Department of Mathematics and Information, Gachon University, Gyeonggi-Do, 4Korea Electronics Technology Institute, Medical IT Technology, Gyeonggi-Do, Republic of KoreaAbstract: A novel clinical glucose biosensor fabricated using functionalized metalloid-polymer (silver-silica coated with polyethylene glycol) hybrid nanoparticles on the surface of a graphene oxide nanosheet is reported. The cyclic voltammetric response of glucose oxidase modification on the surface of a functionalized graphene oxide electrode showed a surface-confined reaction and an effective redox potential near zero volts, with a wide linearity of 0.1&ndash;20 mM and a sensitivity of 7.66 &micro;A mM-1 cm-2. The functionalized graphene oxide electrode showed a better electrocatalytic response toward oxidation of H2O2 and reduction of oxygen. The practical applicability of the functionalized graphene oxide electrode was demonstrated by measuring the peak current against multiple urine and serum samples from diabetic patients. This new hybrid nanoarchitecture combining a three-dimensional metalloid-polymer hybrid and two-dimensional graphene oxide provided a thin solid laminate on the electrode surface. The easy fabrication process and retention of bioactive immobilized enzymes on the functionalized graphene oxide electrode could potentially be extended to detection of other biomolecules, and have broad applications in electrochemical biosensing.Keywords: clinical diagnostics, glucose biosensor, metalloid-polymer nanoparticles, glucose oxidase, cyclic voltammetry
format	article
author	Veerapandian M Seo YT Shin H Yun K Lee MH
author_facet	Veerapandian M Seo YT Shin H Yun K Lee MH
author_sort	Veerapandian M
title	Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples
title_short	Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples
title_full	Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples
title_fullStr	Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples
title_full_unstemmed	Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples
title_sort	functionalized graphene oxide for clinical glucose biosensing in urine and serum samples
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/a03f8bfdeac349c6b18cccb1391e4cc6
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Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

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