The application of EDTA in drug delivery systems: doxorubicin liposomes loaded via NH4EDTA gradient

Yanzhi Song,1 Zhenjun Huang,1 Yang Song,2 Qingjing Tian,1 Xinrong Liu,1 Zhennan She,1 Jiao Jiao,1 Eliza Lu,3 Yihui Deng11College of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People’s Republic of China; 2Jiangsu Hansoh Pharmaceutical Co., Ltd., Lianyungang, People’s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Song YZ, Huang ZJ, Song Y, Tian QJ, Liu XR, She ZN, Jiao J, Lu E, Deng YH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a0454d34a5b447a8ae086e35eb129ec8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a0454d34a5b447a8ae086e35eb129ec8
record_format	dspace
spelling	oai:doaj.org-article:a0454d34a5b447a8ae086e35eb129ec82021-12-02T00:37:18ZThe application of EDTA in drug delivery systems: doxorubicin liposomes loaded via NH4EDTA gradient1178-2013https://doaj.org/article/a0454d34a5b447a8ae086e35eb129ec82014-08-01T00:00:00Zhttp://www.dovepress.com/the-application-of-edta-in-drug-delivery-systems-doxorubicin-liposomes-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Yanzhi Song,1 Zhenjun Huang,1 Yang Song,2 Qingjing Tian,1 Xinrong Liu,1 Zhennan She,1 Jiao Jiao,1 Eliza Lu,3 Yihui Deng11College of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People’s Republic of China; 2Jiangsu Hansoh Pharmaceutical Co., Ltd., Lianyungang, People’s Republic of China; 3Livzon Mabpharm Inc., Zhuhai, People’s Republic of ChinaAbstract: The applications of ethylenediaminetetraacetic acid (EDTA) have been expanded from the treatment of heavy metal poisoning to chelation therapies for atherosclerosis, heart disease, and cancers, in which EDTA reduces morbidity and mortality by chelating toxic metal ions. In this study, EDTA was used in a drug delivery system by adopting an NH4EDTA gradient method to load doxorubicin into liposomes with the goal of increasing therapeutic effects and decreasing drug-related cytotoxicity. The particle size of the optimum NH4EDTA gradient liposomes was 79.4±1.87 nm, and the entrapment efficiency was 95.54%±0.59%. In vitro studies revealed that liposomes prepared using an NH4EDTA gradient possessed long-term stability and delayed drug release. The in vivo studies also showed the superiority of the new doxorubicin formulation. Compared with an equivalent drug dose (5 mg/kg) prepared by (NH4)2SO4 gradient, NH4EDTA gradient liposomes showed no significant differences in tumor inhibition ratio, but cardiotoxicity and liposome-related immune organ damage were lower, and no drug-related deaths were observed. These results show that use of the NH4EDTA gradient method to load doxorubicin into liposomes could significantly reduce drug toxicity without influencing antitumor activity.Keywords: NH4EDTA, liposome, doxorubicin, ion gradient, antitumor activity, toxicitySong YZHuang ZJSong YTian QJLiu XRShe ZNJiao JLu EDeng YHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3611-3621 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Song YZ Huang ZJ Song Y Tian QJ Liu XR She ZN Jiao J Lu E Deng YH The application of EDTA in drug delivery systems: doxorubicin liposomes loaded via NH4EDTA gradient
description	Yanzhi Song,1 Zhenjun Huang,1 Yang Song,2 Qingjing Tian,1 Xinrong Liu,1 Zhennan She,1 Jiao Jiao,1 Eliza Lu,3 Yihui Deng11College of Pharmacy, Shenyang Pharmaceutical University, Shenyang, People’s Republic of China; 2Jiangsu Hansoh Pharmaceutical Co., Ltd., Lianyungang, People’s Republic of China; 3Livzon Mabpharm Inc., Zhuhai, People’s Republic of ChinaAbstract: The applications of ethylenediaminetetraacetic acid (EDTA) have been expanded from the treatment of heavy metal poisoning to chelation therapies for atherosclerosis, heart disease, and cancers, in which EDTA reduces morbidity and mortality by chelating toxic metal ions. In this study, EDTA was used in a drug delivery system by adopting an NH4EDTA gradient method to load doxorubicin into liposomes with the goal of increasing therapeutic effects and decreasing drug-related cytotoxicity. The particle size of the optimum NH4EDTA gradient liposomes was 79.4±1.87 nm, and the entrapment efficiency was 95.54%±0.59%. In vitro studies revealed that liposomes prepared using an NH4EDTA gradient possessed long-term stability and delayed drug release. The in vivo studies also showed the superiority of the new doxorubicin formulation. Compared with an equivalent drug dose (5 mg/kg) prepared by (NH4)2SO4 gradient, NH4EDTA gradient liposomes showed no significant differences in tumor inhibition ratio, but cardiotoxicity and liposome-related immune organ damage were lower, and no drug-related deaths were observed. These results show that use of the NH4EDTA gradient method to load doxorubicin into liposomes could significantly reduce drug toxicity without influencing antitumor activity.Keywords: NH4EDTA, liposome, doxorubicin, ion gradient, antitumor activity, toxicity
format	article
author	Song YZ Huang ZJ Song Y Tian QJ Liu XR She ZN Jiao J Lu E Deng YH
author_facet	Song YZ Huang ZJ Song Y Tian QJ Liu XR She ZN Jiao J Lu E Deng YH
author_sort	Song YZ
title	The application of EDTA in drug delivery systems: doxorubicin liposomes loaded via NH4EDTA gradient
title_short	The application of EDTA in drug delivery systems: doxorubicin liposomes loaded via NH4EDTA gradient
title_full	The application of EDTA in drug delivery systems: doxorubicin liposomes loaded via NH4EDTA gradient
title_fullStr	The application of EDTA in drug delivery systems: doxorubicin liposomes loaded via NH4EDTA gradient
title_full_unstemmed	The application of EDTA in drug delivery systems: doxorubicin liposomes loaded via NH4EDTA gradient
title_sort	application of edta in drug delivery systems: doxorubicin liposomes loaded via nh4edta gradient
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/a0454d34a5b447a8ae086e35eb129ec8
work_keys_str_mv	AT songyz theapplicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT huangzj theapplicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT songy theapplicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT tianqj theapplicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT liuxr theapplicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT shezn theapplicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT jiaoj theapplicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT lue theapplicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT dengyh theapplicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT songyz applicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT huangzj applicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT songy applicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT tianqj applicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT liuxr applicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT shezn applicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT jiaoj applicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT lue applicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient AT dengyh applicationofedtaindrugdeliverysystemsdoxorubicinliposomesloadedvianh4edtagradient
_version_	1718403636445315072

The application of EDTA in drug delivery systems: doxorubicin liposomes loaded via NH4EDTA gradient

Ejemplares similares