A Three-Dimensional Co-Culture Model for Rheumatoid Arthritis Pannus Tissue
Three-dimensional (3D) co-culture models have closer physiological cell composition and behavior than traditional 2D culture. They exhibit pharmacological effects like in vivo responses, and therefore serve as a high-throughput drug screening model to evaluate drug efficacy and safety in vitro. In t...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:a05aa82a38aa483ab7c6e7baae2a60702021-11-18T17:04:34ZA Three-Dimensional Co-Culture Model for Rheumatoid Arthritis Pannus Tissue2296-418510.3389/fbioe.2021.764212https://doaj.org/article/a05aa82a38aa483ab7c6e7baae2a60702021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.764212/fullhttps://doaj.org/toc/2296-4185Three-dimensional (3D) co-culture models have closer physiological cell composition and behavior than traditional 2D culture. They exhibit pharmacological effects like in vivo responses, and therefore serve as a high-throughput drug screening model to evaluate drug efficacy and safety in vitro. In this study, we created a 3D co-culture environment to mimic pathological characteristics of rheumatoid arthritis (RA) pannus tissue. 3D scaffold was constructed by bioprinting technology with synovial fibroblasts (MH7A), vascular endothelial cells (EA.hy 926) and gelatin/alginate hydrogels. Cell viability was observed during 7-day culture and the proliferation rate of co-culture cells showed a stable increase stage. Cell-cell interactions were evaluated in the 3D printed scaffold and we found that spheroid size increased with time. TNF-α stimulated MH7A and EA.hy 926 in 3D pannus model showed higher vascular endothelial growth factor (VEGF) and angiopoietin (ANG) protein expression over time. For drug validation, methotrexate (MTX) was used to examine inhibition effects of angiogenesis in 3D pannus co-culture model. In conclusion, this 3D co-culture pannus model with biological characteristics may help the development of anti-RA drug research.Jietao LinJietao LinJietao LinAntonia RuJia SunAntonia RuJia SunAntonia RuJia SunJian LiJian LiJian LiTianying YuanWenxiang ChengWenxiang ChengWenxiang ChengLiqing KeLiqing KeLiqing KeJianhai ChenJianhai ChenJianhai ChenWei SunShengli MiPeng ZhangPeng ZhangPeng ZhangFrontiers Media S.A.articlerheumatoid arthritis3D bioprintingtissue engineeringpannus tissue modeldrug screeningBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
rheumatoid arthritis 3D bioprinting tissue engineering pannus tissue model drug screening Biotechnology TP248.13-248.65 |
| spellingShingle |
rheumatoid arthritis 3D bioprinting tissue engineering pannus tissue model drug screening Biotechnology TP248.13-248.65 Jietao Lin Jietao Lin Jietao Lin Antonia RuJia Sun Antonia RuJia Sun Antonia RuJia Sun Jian Li Jian Li Jian Li Tianying Yuan Wenxiang Cheng Wenxiang Cheng Wenxiang Cheng Liqing Ke Liqing Ke Liqing Ke Jianhai Chen Jianhai Chen Jianhai Chen Wei Sun Shengli Mi Peng Zhang Peng Zhang Peng Zhang A Three-Dimensional Co-Culture Model for Rheumatoid Arthritis Pannus Tissue |
| description |
Three-dimensional (3D) co-culture models have closer physiological cell composition and behavior than traditional 2D culture. They exhibit pharmacological effects like in vivo responses, and therefore serve as a high-throughput drug screening model to evaluate drug efficacy and safety in vitro. In this study, we created a 3D co-culture environment to mimic pathological characteristics of rheumatoid arthritis (RA) pannus tissue. 3D scaffold was constructed by bioprinting technology with synovial fibroblasts (MH7A), vascular endothelial cells (EA.hy 926) and gelatin/alginate hydrogels. Cell viability was observed during 7-day culture and the proliferation rate of co-culture cells showed a stable increase stage. Cell-cell interactions were evaluated in the 3D printed scaffold and we found that spheroid size increased with time. TNF-α stimulated MH7A and EA.hy 926 in 3D pannus model showed higher vascular endothelial growth factor (VEGF) and angiopoietin (ANG) protein expression over time. For drug validation, methotrexate (MTX) was used to examine inhibition effects of angiogenesis in 3D pannus co-culture model. In conclusion, this 3D co-culture pannus model with biological characteristics may help the development of anti-RA drug research. |
| format |
article |
| author |
Jietao Lin Jietao Lin Jietao Lin Antonia RuJia Sun Antonia RuJia Sun Antonia RuJia Sun Jian Li Jian Li Jian Li Tianying Yuan Wenxiang Cheng Wenxiang Cheng Wenxiang Cheng Liqing Ke Liqing Ke Liqing Ke Jianhai Chen Jianhai Chen Jianhai Chen Wei Sun Shengli Mi Peng Zhang Peng Zhang Peng Zhang |
| author_facet |
Jietao Lin Jietao Lin Jietao Lin Antonia RuJia Sun Antonia RuJia Sun Antonia RuJia Sun Jian Li Jian Li Jian Li Tianying Yuan Wenxiang Cheng Wenxiang Cheng Wenxiang Cheng Liqing Ke Liqing Ke Liqing Ke Jianhai Chen Jianhai Chen Jianhai Chen Wei Sun Shengli Mi Peng Zhang Peng Zhang Peng Zhang |
| author_sort |
Jietao Lin |
| title |
A Three-Dimensional Co-Culture Model for Rheumatoid Arthritis Pannus Tissue |
| title_short |
A Three-Dimensional Co-Culture Model for Rheumatoid Arthritis Pannus Tissue |
| title_full |
A Three-Dimensional Co-Culture Model for Rheumatoid Arthritis Pannus Tissue |
| title_fullStr |
A Three-Dimensional Co-Culture Model for Rheumatoid Arthritis Pannus Tissue |
| title_full_unstemmed |
A Three-Dimensional Co-Culture Model for Rheumatoid Arthritis Pannus Tissue |
| title_sort |
three-dimensional co-culture model for rheumatoid arthritis pannus tissue |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/a05aa82a38aa483ab7c6e7baae2a6070 |
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