The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking

ABSTRACT Natural products are the richest source of chemical compounds for drug discovery. Particularly, bacterial secondary metabolites are in the spotlight due to advances in genome sequencing and mining, as well as for the potential of biosynthetic pathway manipulation to awake silent (cryptic) g...

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Autores principales:	Daniela B. B. Trivella, Rafael de Felicio
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	bacterial secondary metabolites biosynthesis cryptic gene clusters drug discovery genome mining mass spectrometry Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a05cc3dfd67d45fc9a3bf649d1b395cf
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id	oai:doaj.org-article:a05cc3dfd67d45fc9a3bf649d1b395cf
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spelling	oai:doaj.org-article:a05cc3dfd67d45fc9a3bf649d1b395cf2021-12-02T18:15:47ZThe Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking10.1128/mSystems.00160-172379-5077https://doaj.org/article/a05cc3dfd67d45fc9a3bf649d1b395cf2018-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00160-17https://doaj.org/toc/2379-5077ABSTRACT Natural products are the richest source of chemical compounds for drug discovery. Particularly, bacterial secondary metabolites are in the spotlight due to advances in genome sequencing and mining, as well as for the potential of biosynthetic pathway manipulation to awake silent (cryptic) gene clusters under laboratory cultivation. Further progress in compound detection, such as the development of the tandem mass spectrometry (MS/MS) molecular networking approach, has contributed to the discovery of novel bacterial natural products. The latter can be applied directly to bacterial crude extracts for identifying and dereplicating known compounds, therefore assisting the prioritization of extracts containing novel natural products, for example. In our opinion, these three approaches—genome mining, silent pathway induction, and MS-based molecular networking—compose the tripod for modern bacterial natural product discovery and will be discussed in this perspective.Daniela B. B. TrivellaRafael de FelicioAmerican Society for Microbiologyarticlebacterial secondary metabolitesbiosynthesiscryptic gene clustersdrug discoverygenome miningmass spectrometryMicrobiologyQR1-502ENmSystems, Vol 3, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial secondary metabolites biosynthesis cryptic gene clusters drug discovery genome mining mass spectrometry Microbiology QR1-502
spellingShingle	bacterial secondary metabolites biosynthesis cryptic gene clusters drug discovery genome mining mass spectrometry Microbiology QR1-502 Daniela B. B. Trivella Rafael de Felicio The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking
description	ABSTRACT Natural products are the richest source of chemical compounds for drug discovery. Particularly, bacterial secondary metabolites are in the spotlight due to advances in genome sequencing and mining, as well as for the potential of biosynthetic pathway manipulation to awake silent (cryptic) gene clusters under laboratory cultivation. Further progress in compound detection, such as the development of the tandem mass spectrometry (MS/MS) molecular networking approach, has contributed to the discovery of novel bacterial natural products. The latter can be applied directly to bacterial crude extracts for identifying and dereplicating known compounds, therefore assisting the prioritization of extracts containing novel natural products, for example. In our opinion, these three approaches—genome mining, silent pathway induction, and MS-based molecular networking—compose the tripod for modern bacterial natural product discovery and will be discussed in this perspective.
format	article
author	Daniela B. B. Trivella Rafael de Felicio
author_facet	Daniela B. B. Trivella Rafael de Felicio
author_sort	Daniela B. B. Trivella
title	The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking
title_short	The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking
title_full	The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking
title_fullStr	The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking
title_full_unstemmed	The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking
title_sort	tripod for bacterial natural product discovery: genome mining, silent pathway induction, and mass spectrometry-based molecular networking
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/a05cc3dfd67d45fc9a3bf649d1b395cf
work_keys_str_mv	AT danielabbtrivella thetripodforbacterialnaturalproductdiscoverygenomeminingsilentpathwayinductionandmassspectrometrybasedmolecularnetworking AT rafaeldefelicio thetripodforbacterialnaturalproductdiscoverygenomeminingsilentpathwayinductionandmassspectrometrybasedmolecularnetworking AT danielabbtrivella tripodforbacterialnaturalproductdiscoverygenomeminingsilentpathwayinductionandmassspectrometrybasedmolecularnetworking AT rafaeldefelicio tripodforbacterialnaturalproductdiscoverygenomeminingsilentpathwayinductionandmassspectrometrybasedmolecularnetworking
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The Tripod for Bacterial Natural Product Discovery: Genome Mining, Silent Pathway Induction, and Mass Spectrometry-Based Molecular Networking

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