Lack of association of –863C/A (rs1800630) polymorphism of tumor necrosis factor-α gene with rheumatoid arthritis

Introduction Multifunctional pro-inflammatory cytokine tumor necrosis factor-α (TNF-α) has been implicated in a variety of inflammatory diseases including rheumatoid arthritis (RA). TNF-α polymorphisms are mostly located in its promoter region and play a significant role in disease susceptibility an...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tayyaba Sadaf, Peter John, Attya Bhatti, Javaid M. Malik
Formato:	article
Lenguaje:	EN
Publicado:	Termedia Publishing House 2019
Materias:	rheumatoid arthritis polymorphism association study tumor necrosis factor-alpha Medicine R
Acceso en línea:	https://doaj.org/article/a0694443d9a44e6c90e0cbb7d8224201
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a0694443d9a44e6c90e0cbb7d8224201
record_format	dspace
spelling	oai:doaj.org-article:a0694443d9a44e6c90e0cbb7d82242012021-12-02T19:15:42ZLack of association of –863C/A (rs1800630) polymorphism of tumor necrosis factor-α gene with rheumatoid arthritis1734-19221896-915110.5114/aoms.2018.76946https://doaj.org/article/a0694443d9a44e6c90e0cbb7d82242012019-03-01T00:00:00Zhttps://www.archivesofmedicalscience.com/Lack-of-association-of-863C-A-rs1800630-polymorphism-of-tumor-necrosis-factor-gene,68764,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction Multifunctional pro-inflammatory cytokine tumor necrosis factor-α (TNF-α) has been implicated in a variety of inflammatory diseases including rheumatoid arthritis (RA). TNF-α polymorphisms are mostly located in its promoter region and play a significant role in disease susceptibility and severity. We therefore sought to investigate TNFA –863C/A (rs1800630) polymorphism association with RA activity in our Pakistani study group. Material and methods A total of 268 human subjects were enrolled. Among them, 134 were RA patients and 134 were controls. In this study the physical parameters of RA patients were collected, and the disease activity was measured by DAS28. The genotypes were determined following the allele-specific PCR along with the pre-requisite internal amplification controls. Subsequently, data were analyzed statistically for any significant association including χ 2 /Fisher’s exact test using GraphPad prism 6 software. Results We found that the TNF-α –863 C/A (rs1800630) variant was not differentially segregated between cases and controls in either genotype frequency, with χ2 of 2.771 and a p-value of 0.2502, or allele frequency, with χ2 of 2.741 and a p-value of 0.0978, with an odds ratio (95% CI) of 0.7490 (0.5317–1.055). Conclusions The lack of positive association of TNF-α –863(rs1800630) polymorphism in our study group implies that TNF-α –863 polymorphism is not a susceptible marker to RA and cannot serve as a genetic factor for screening RA patients in Pakistan. There might be other factors that may influence disease susceptibility. However, further investigations on additional larger and multi-regional population samples are required to determine the consequences of genetic variations for disease prognosis.Tayyaba SadafPeter JohnAttya BhattiJavaid M. MalikTermedia Publishing Housearticlerheumatoid arthritispolymorphismassociation studytumor necrosis factor-alphaMedicineRENArchives of Medical Science, Vol 15, Iss 2, Pp 531-536 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	rheumatoid arthritis polymorphism association study tumor necrosis factor-alpha Medicine R
spellingShingle	rheumatoid arthritis polymorphism association study tumor necrosis factor-alpha Medicine R Tayyaba Sadaf Peter John Attya Bhatti Javaid M. Malik Lack of association of –863C/A (rs1800630) polymorphism of tumor necrosis factor-α gene with rheumatoid arthritis
description	Introduction Multifunctional pro-inflammatory cytokine tumor necrosis factor-α (TNF-α) has been implicated in a variety of inflammatory diseases including rheumatoid arthritis (RA). TNF-α polymorphisms are mostly located in its promoter region and play a significant role in disease susceptibility and severity. We therefore sought to investigate TNFA –863C/A (rs1800630) polymorphism association with RA activity in our Pakistani study group. Material and methods A total of 268 human subjects were enrolled. Among them, 134 were RA patients and 134 were controls. In this study the physical parameters of RA patients were collected, and the disease activity was measured by DAS28. The genotypes were determined following the allele-specific PCR along with the pre-requisite internal amplification controls. Subsequently, data were analyzed statistically for any significant association including χ 2 /Fisher’s exact test using GraphPad prism 6 software. Results We found that the TNF-α –863 C/A (rs1800630) variant was not differentially segregated between cases and controls in either genotype frequency, with χ2 of 2.771 and a p-value of 0.2502, or allele frequency, with χ2 of 2.741 and a p-value of 0.0978, with an odds ratio (95% CI) of 0.7490 (0.5317–1.055). Conclusions The lack of positive association of TNF-α –863(rs1800630) polymorphism in our study group implies that TNF-α –863 polymorphism is not a susceptible marker to RA and cannot serve as a genetic factor for screening RA patients in Pakistan. There might be other factors that may influence disease susceptibility. However, further investigations on additional larger and multi-regional population samples are required to determine the consequences of genetic variations for disease prognosis.
format	article
author	Tayyaba Sadaf Peter John Attya Bhatti Javaid M. Malik
author_facet	Tayyaba Sadaf Peter John Attya Bhatti Javaid M. Malik
author_sort	Tayyaba Sadaf
title	Lack of association of –863C/A (rs1800630) polymorphism of tumor necrosis factor-α gene with rheumatoid arthritis
title_short	Lack of association of –863C/A (rs1800630) polymorphism of tumor necrosis factor-α gene with rheumatoid arthritis
title_full	Lack of association of –863C/A (rs1800630) polymorphism of tumor necrosis factor-α gene with rheumatoid arthritis
title_fullStr	Lack of association of –863C/A (rs1800630) polymorphism of tumor necrosis factor-α gene with rheumatoid arthritis
title_full_unstemmed	Lack of association of –863C/A (rs1800630) polymorphism of tumor necrosis factor-α gene with rheumatoid arthritis
title_sort	lack of association of –863c/a (rs1800630) polymorphism of tumor necrosis factor-α gene with rheumatoid arthritis
publisher	Termedia Publishing House
publishDate	2019
url	https://doaj.org/article/a0694443d9a44e6c90e0cbb7d8224201
work_keys_str_mv	AT tayyabasadaf lackofassociationof863cars1800630polymorphismoftumornecrosisfactoragenewithrheumatoidarthritis AT peterjohn lackofassociationof863cars1800630polymorphismoftumornecrosisfactoragenewithrheumatoidarthritis AT attyabhatti lackofassociationof863cars1800630polymorphismoftumornecrosisfactoragenewithrheumatoidarthritis AT javaidmmalik lackofassociationof863cars1800630polymorphismoftumornecrosisfactoragenewithrheumatoidarthritis
_version_	1718376989113450496

Lack of association of –863C/A (rs1800630) polymorphism of tumor necrosis factor-α gene with rheumatoid arthritis

Ejemplares similares