RPLP1, a crucial ribosomal protein for embryonic development of the nervous system.

Ribosomal proteins are pivotal to development and tissue homeostasis. RP Large P1 (Rplp1) overexpression is associated with tumorigenesis. However, the physiological function of Rplp1 in mammalian development remains unknown. In this study, we disrupted Rplp1 in the mouse germline and central nervou...

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Autores principales:	Laura Perucho, Ana Artero-Castro, Sergi Guerrero, Santiago Ramón y Cajal, Matilde E LLeonart, Zhao-Qi Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a075bf2702d748539f3f182f00c201da
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spelling	oai:doaj.org-article:a075bf2702d748539f3f182f00c201da2021-11-11T08:21:47ZRPLP1, a crucial ribosomal protein for embryonic development of the nervous system.1932-620310.1371/journal.pone.0099956https://doaj.org/article/a075bf2702d748539f3f182f00c201da2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24959908/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Ribosomal proteins are pivotal to development and tissue homeostasis. RP Large P1 (Rplp1) overexpression is associated with tumorigenesis. However, the physiological function of Rplp1 in mammalian development remains unknown. In this study, we disrupted Rplp1 in the mouse germline and central nervous system (Rplp1CNSΔ). Rplp1 heterozygosity caused body size reductions, male infertility, systemic abnormalities in various tissues and a high frequency of early postnatal death. Rplp1CNSΔ newborn mice exhibited perinatal lethality and brain atrophy with size reductions of the neocortex, midbrain and ganglionic eminence. The Rplp1 knockout neocortex exhibited progenitor cell proliferation arrest and apoptosis due to the dysregulation of key cell cycle and apoptosis regulators (cyclin A, cyclin E, p21CIP1, p27KIP1, p53). Similarly, Rplp1 deletion in pMEFs led to proliferation arrest and premature senescence. Importantly, Rplp1 deletion in primary mouse embryonic fibroblasts did not alter global protein synthesis, but did change the expression patterns of specific protein subsets involved in protein folding and the unfolded protein response, cell death, protein transport and signal transduction, among others. Altogether, we demonstrated that the translation "fine-tuning" exerted by Rplp1 is essential for embryonic and brain development and for proper cell proliferation.Laura PeruchoAna Artero-CastroSergi GuerreroSantiago Ramón y CajalMatilde E LLeonartZhao-Qi WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99956 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura Perucho Ana Artero-Castro Sergi Guerrero Santiago Ramón y Cajal Matilde E LLeonart Zhao-Qi Wang RPLP1, a crucial ribosomal protein for embryonic development of the nervous system.
description	Ribosomal proteins are pivotal to development and tissue homeostasis. RP Large P1 (Rplp1) overexpression is associated with tumorigenesis. However, the physiological function of Rplp1 in mammalian development remains unknown. In this study, we disrupted Rplp1 in the mouse germline and central nervous system (Rplp1CNSΔ). Rplp1 heterozygosity caused body size reductions, male infertility, systemic abnormalities in various tissues and a high frequency of early postnatal death. Rplp1CNSΔ newborn mice exhibited perinatal lethality and brain atrophy with size reductions of the neocortex, midbrain and ganglionic eminence. The Rplp1 knockout neocortex exhibited progenitor cell proliferation arrest and apoptosis due to the dysregulation of key cell cycle and apoptosis regulators (cyclin A, cyclin E, p21CIP1, p27KIP1, p53). Similarly, Rplp1 deletion in pMEFs led to proliferation arrest and premature senescence. Importantly, Rplp1 deletion in primary mouse embryonic fibroblasts did not alter global protein synthesis, but did change the expression patterns of specific protein subsets involved in protein folding and the unfolded protein response, cell death, protein transport and signal transduction, among others. Altogether, we demonstrated that the translation "fine-tuning" exerted by Rplp1 is essential for embryonic and brain development and for proper cell proliferation.
format	article
author	Laura Perucho Ana Artero-Castro Sergi Guerrero Santiago Ramón y Cajal Matilde E LLeonart Zhao-Qi Wang
author_facet	Laura Perucho Ana Artero-Castro Sergi Guerrero Santiago Ramón y Cajal Matilde E LLeonart Zhao-Qi Wang
author_sort	Laura Perucho
title	RPLP1, a crucial ribosomal protein for embryonic development of the nervous system.
title_short	RPLP1, a crucial ribosomal protein for embryonic development of the nervous system.
title_full	RPLP1, a crucial ribosomal protein for embryonic development of the nervous system.
title_fullStr	RPLP1, a crucial ribosomal protein for embryonic development of the nervous system.
title_full_unstemmed	RPLP1, a crucial ribosomal protein for embryonic development of the nervous system.
title_sort	rplp1, a crucial ribosomal protein for embryonic development of the nervous system.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/a075bf2702d748539f3f182f00c201da
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RPLP1, a crucial ribosomal protein for embryonic development of the nervous system.

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