Gene transfer using micellar nanovectors inhibits choroidal neovascularization in vivo.

<h4>Purpose</h4>Age-related macular degeneration caused by choroidal neovascularization (CNV) remains difficult to be treated despite the recent advent of several treatment options. In this study, we investigated the in vivo angiogenic control by intravenous injection of polyion complex...

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Autores principales:	Aya Iriyama, Makoto Oba, Takehiko Ishii, Nobuhiro Nishiyama, Kazunori Kataoka, Yasuhiro Tamaki, Yasuo Yanagi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a083abcc74214c24aa672ce024fc0d0b
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spelling	oai:doaj.org-article:a083abcc74214c24aa672ce024fc0d0b2021-11-18T07:33:00ZGene transfer using micellar nanovectors inhibits choroidal neovascularization in vivo.1932-620310.1371/journal.pone.0028560https://doaj.org/article/a083abcc74214c24aa672ce024fc0d0b2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22162776/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Purpose</h4>Age-related macular degeneration caused by choroidal neovascularization (CNV) remains difficult to be treated despite the recent advent of several treatment options. In this study, we investigated the in vivo angiogenic control by intravenous injection of polyion complex (PIC) micelle encapsulating plasmid DNA (pDNA) using a mice CNV model.<h4>Methods</h4>The transfection efficiency of the PIC micelle was investigated using the laser-induced CNV in eight-week-old male C57 BJ/6 mice. Firstly, each mouse received intravenous injection of micelle encapsulating pDNA of Yellow Fluorescent Protein (pYFP) on days 1,3 and 5. The expression of YFP was analyzed using fluorescein microscopy and western blotting analysis. In the next experiments, each mouse received intravenous injection of micelle encapsulating pDNA of soluble Fms-like tyrosine kinase-1 (psFlt-1) 1,3 and 5 days after the induction of CNV and the CNV lesion was analyzed by choroidal flatmounts on day 7.<h4>Results</h4>Fluorescein microscopy and western blotting analysis revealed that the expression of YFP was confirmed in the CNV area after injection of the PIC micelle, but the expression was not detected neither in mice that received naked pDNA nor those without CNV. Furthermore, the CNV area in the mice that received intravenous injection of the psFlt-1-encapsulated PIC micelle was significantly reduced by 65% compared to that in control mice (p<0.01).<h4>Conclusions</h4>Transfection of sFlt-1 with the PIC micelle by intravenous injection to mice CNV models showed significant inhibition of CNV. The current results revealed the significant potential of nonviral gene therapy for regulation of CNV using the PIC micelle encapsulating pDNA.Aya IriyamaMakoto ObaTakehiko IshiiNobuhiro NishiyamaKazunori KataokaYasuhiro TamakiYasuo YanagiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28560 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aya Iriyama Makoto Oba Takehiko Ishii Nobuhiro Nishiyama Kazunori Kataoka Yasuhiro Tamaki Yasuo Yanagi Gene transfer using micellar nanovectors inhibits choroidal neovascularization in vivo.
description	<h4>Purpose</h4>Age-related macular degeneration caused by choroidal neovascularization (CNV) remains difficult to be treated despite the recent advent of several treatment options. In this study, we investigated the in vivo angiogenic control by intravenous injection of polyion complex (PIC) micelle encapsulating plasmid DNA (pDNA) using a mice CNV model.<h4>Methods</h4>The transfection efficiency of the PIC micelle was investigated using the laser-induced CNV in eight-week-old male C57 BJ/6 mice. Firstly, each mouse received intravenous injection of micelle encapsulating pDNA of Yellow Fluorescent Protein (pYFP) on days 1,3 and 5. The expression of YFP was analyzed using fluorescein microscopy and western blotting analysis. In the next experiments, each mouse received intravenous injection of micelle encapsulating pDNA of soluble Fms-like tyrosine kinase-1 (psFlt-1) 1,3 and 5 days after the induction of CNV and the CNV lesion was analyzed by choroidal flatmounts on day 7.<h4>Results</h4>Fluorescein microscopy and western blotting analysis revealed that the expression of YFP was confirmed in the CNV area after injection of the PIC micelle, but the expression was not detected neither in mice that received naked pDNA nor those without CNV. Furthermore, the CNV area in the mice that received intravenous injection of the psFlt-1-encapsulated PIC micelle was significantly reduced by 65% compared to that in control mice (p<0.01).<h4>Conclusions</h4>Transfection of sFlt-1 with the PIC micelle by intravenous injection to mice CNV models showed significant inhibition of CNV. The current results revealed the significant potential of nonviral gene therapy for regulation of CNV using the PIC micelle encapsulating pDNA.
format	article
author	Aya Iriyama Makoto Oba Takehiko Ishii Nobuhiro Nishiyama Kazunori Kataoka Yasuhiro Tamaki Yasuo Yanagi
author_facet	Aya Iriyama Makoto Oba Takehiko Ishii Nobuhiro Nishiyama Kazunori Kataoka Yasuhiro Tamaki Yasuo Yanagi
author_sort	Aya Iriyama
title	Gene transfer using micellar nanovectors inhibits choroidal neovascularization in vivo.
title_short	Gene transfer using micellar nanovectors inhibits choroidal neovascularization in vivo.
title_full	Gene transfer using micellar nanovectors inhibits choroidal neovascularization in vivo.
title_fullStr	Gene transfer using micellar nanovectors inhibits choroidal neovascularization in vivo.
title_full_unstemmed	Gene transfer using micellar nanovectors inhibits choroidal neovascularization in vivo.
title_sort	gene transfer using micellar nanovectors inhibits choroidal neovascularization in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a083abcc74214c24aa672ce024fc0d0b
work_keys_str_mv	AT ayairiyama genetransferusingmicellarnanovectorsinhibitschoroidalneovascularizationinvivo AT makotooba genetransferusingmicellarnanovectorsinhibitschoroidalneovascularizationinvivo AT takehikoishii genetransferusingmicellarnanovectorsinhibitschoroidalneovascularizationinvivo AT nobuhironishiyama genetransferusingmicellarnanovectorsinhibitschoroidalneovascularizationinvivo AT kazunorikataoka genetransferusingmicellarnanovectorsinhibitschoroidalneovascularizationinvivo AT yasuhirotamaki genetransferusingmicellarnanovectorsinhibitschoroidalneovascularizationinvivo AT yasuoyanagi genetransferusingmicellarnanovectorsinhibitschoroidalneovascularizationinvivo
_version_	1718423303986610176

Gene transfer using micellar nanovectors inhibits choroidal neovascularization in vivo.

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