A sensitive acupuncture needle microsensor for real-time monitoring of nitric oxide in acupoints of rats

Abstract This study reports an acupuncture needle modified with an iron-porphyrin functionalized graphene composite (FGPC) for real-time monitoring of nitric oxide (NO) release in acupoints of rats. A gold film was first deposited to the needle surface to enhance the conductivity. The FGPC was prepa...

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Autores principales:	Lina Tang, Yutao Li, Hui Xie, Qing Shu, Fan Yang, Yan-ling Liu, Fengxia Liang, Hua Wang, Weihua Huang, Guo-Jun Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a08a6647449b4a3480728c438259d45b
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spelling	oai:doaj.org-article:a08a6647449b4a3480728c438259d45b2021-12-02T12:30:27ZA sensitive acupuncture needle microsensor for real-time monitoring of nitric oxide in acupoints of rats10.1038/s41598-017-06657-32045-2322https://doaj.org/article/a08a6647449b4a3480728c438259d45b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06657-3https://doaj.org/toc/2045-2322Abstract This study reports an acupuncture needle modified with an iron-porphyrin functionalized graphene composite (FGPC) for real-time monitoring of nitric oxide (NO) release in acupoints of rats. A gold film was first deposited to the needle surface to enhance the conductivity. The FGPC was prepared via hydrothermal synthesis, and subsequently applied to the tip surface of acupuncture needle by electrochemical deposition method. The functionalized needle enabled a specific and sensitive detection of NO based on the favorably catalytic properties of iron-porphyrin and the excellent conductivity of graphene. Amperometric data showed that the needle achieved not only a low detection limit down to 3.2 nM in PBS solution, but also a satisfactory selectivity. Interestingly, the functionalized needle could be inserted into the acupoints of rats for real-time monitoring of NO in vivo. It was found that a remarkable response to NO was respectively obtained in different acupoints when stimulated by L-arginine (L-Arg), revealing that the release of NO was detectable in acupoints. We expect this work would showcase the applications of acupuncture needle in detecting some important signaling molecules in vivo, and exploring the mechanism of acupuncture treatment.Lina TangYutao LiHui XieQing ShuFan YangYan-ling LiuFengxia LiangHua WangWeihua HuangGuo-Jun ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lina Tang Yutao Li Hui Xie Qing Shu Fan Yang Yan-ling Liu Fengxia Liang Hua Wang Weihua Huang Guo-Jun Zhang A sensitive acupuncture needle microsensor for real-time monitoring of nitric oxide in acupoints of rats
description	Abstract This study reports an acupuncture needle modified with an iron-porphyrin functionalized graphene composite (FGPC) for real-time monitoring of nitric oxide (NO) release in acupoints of rats. A gold film was first deposited to the needle surface to enhance the conductivity. The FGPC was prepared via hydrothermal synthesis, and subsequently applied to the tip surface of acupuncture needle by electrochemical deposition method. The functionalized needle enabled a specific and sensitive detection of NO based on the favorably catalytic properties of iron-porphyrin and the excellent conductivity of graphene. Amperometric data showed that the needle achieved not only a low detection limit down to 3.2 nM in PBS solution, but also a satisfactory selectivity. Interestingly, the functionalized needle could be inserted into the acupoints of rats for real-time monitoring of NO in vivo. It was found that a remarkable response to NO was respectively obtained in different acupoints when stimulated by L-arginine (L-Arg), revealing that the release of NO was detectable in acupoints. We expect this work would showcase the applications of acupuncture needle in detecting some important signaling molecules in vivo, and exploring the mechanism of acupuncture treatment.
format	article
author	Lina Tang Yutao Li Hui Xie Qing Shu Fan Yang Yan-ling Liu Fengxia Liang Hua Wang Weihua Huang Guo-Jun Zhang
author_facet	Lina Tang Yutao Li Hui Xie Qing Shu Fan Yang Yan-ling Liu Fengxia Liang Hua Wang Weihua Huang Guo-Jun Zhang
author_sort	Lina Tang
title	A sensitive acupuncture needle microsensor for real-time monitoring of nitric oxide in acupoints of rats
title_short	A sensitive acupuncture needle microsensor for real-time monitoring of nitric oxide in acupoints of rats
title_full	A sensitive acupuncture needle microsensor for real-time monitoring of nitric oxide in acupoints of rats
title_fullStr	A sensitive acupuncture needle microsensor for real-time monitoring of nitric oxide in acupoints of rats
title_full_unstemmed	A sensitive acupuncture needle microsensor for real-time monitoring of nitric oxide in acupoints of rats
title_sort	sensitive acupuncture needle microsensor for real-time monitoring of nitric oxide in acupoints of rats
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a08a6647449b4a3480728c438259d45b
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A sensitive acupuncture needle microsensor for real-time monitoring of nitric oxide in acupoints of rats

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