Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo

Yan He,1,* Zhiyun Du,1,* Shijing Ma,1 Yue Liu,2 Dongli Li,1 Huarong Huang,1 Sen Jiang,1 Shupeng Cheng,1 Wenjing Wu,1 Kun Zhang,1 Xi Zheng1,2 1Allan H Conney Laboratory for Anticancer Drug Research, School of Chemical Engineering and Light Industry, Guandong University of Technology, Guangzhou, Peop...

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Autores principales:	He Y, Du Z, Ma S, Liu Y, Li D, Huang H, Jiang S, Cheng S, Wu W, Zhang K, Zheng X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Silver nanoparticle antitumor lung cancer cytotoxicity H1299 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a08bdcf9c65c49bc922b23981b5c083b
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spelling	oai:doaj.org-article:a08bdcf9c65c49bc922b23981b5c083b2021-12-02T07:46:32ZEffects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo1178-2013https://doaj.org/article/a08bdcf9c65c49bc922b23981b5c083b2016-05-01T00:00:00Zhttps://www.dovepress.com/effects-of-green-synthesized-silver-nanoparticles-on-lung-cancer-cells-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yan He,1,* Zhiyun Du,1,* Shijing Ma,1 Yue Liu,2 Dongli Li,1 Huarong Huang,1 Sen Jiang,1 Shupeng Cheng,1 Wenjing Wu,1 Kun Zhang,1 Xi Zheng1,2 1Allan H Conney Laboratory for Anticancer Drug Research, School of Chemical Engineering and Light Industry, Guandong University of Technology, Guangzhou, People’s Republic of China; 2Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, USA *These authors contributed equally to this work Abstract: Silver nanoparticles (AgNPs) have now been recognized as promising therapeutic molecules and are extending their use in cancer diagnosis and therapy. This study demonstrates for the first time the antitumor activity of green-synthesized AgNPs against lung cancer in vitro and in vivo. Cytotoxicity effect was explored on human lung cancer H1299 cells in vitro by MTT and trypan blue assays. Apoptosis was measured by morphological assessment, and nuclear factor-κB (NF-κB) transcriptional activity was determined by a luciferase reporter gene assay. The expressions of phosphorylated stat3, bcl-2, survivin, and caspase-3 were examined by Western blot analysis. AgNPs showed dose-dependent cytotoxicity and stimulation of apoptosis in H1299 cells. The effects on H1299 cells correlated well with the inhibition of NF-κB activity, a decrease in bcl-2, and an increase in caspase-3 and survivin expression. AgNPs significantly suppressed the H1299 tumor growth in a xenograft severe combined immunodeficient (SCID) mouse model. The results demonstrate the anticancer activities of AgNPs, suggesting that they may act as potential beneficial molecules in lung cancer chemoprevention and chemotherapy, especially for early-stage intervention. Keywords: silver nanoparticles, antitumor, lung cancer, cytotoxicity, H1299He YDu ZMa SLiu YLi DHuang HJiang SCheng SWu WZhang KZheng XDove Medical PressarticleSilver nanoparticleantitumorlung cancercytotoxicityH1299Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1879-1887 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Silver nanoparticle antitumor lung cancer cytotoxicity H1299 Medicine (General) R5-920
spellingShingle	Silver nanoparticle antitumor lung cancer cytotoxicity H1299 Medicine (General) R5-920 He Y Du Z Ma S Liu Y Li D Huang H Jiang S Cheng S Wu W Zhang K Zheng X Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo
description	Yan He,1,* Zhiyun Du,1,* Shijing Ma,1 Yue Liu,2 Dongli Li,1 Huarong Huang,1 Sen Jiang,1 Shupeng Cheng,1 Wenjing Wu,1 Kun Zhang,1 Xi Zheng1,2 1Allan H Conney Laboratory for Anticancer Drug Research, School of Chemical Engineering and Light Industry, Guandong University of Technology, Guangzhou, People’s Republic of China; 2Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, USA *These authors contributed equally to this work Abstract: Silver nanoparticles (AgNPs) have now been recognized as promising therapeutic molecules and are extending their use in cancer diagnosis and therapy. This study demonstrates for the first time the antitumor activity of green-synthesized AgNPs against lung cancer in vitro and in vivo. Cytotoxicity effect was explored on human lung cancer H1299 cells in vitro by MTT and trypan blue assays. Apoptosis was measured by morphological assessment, and nuclear factor-κB (NF-κB) transcriptional activity was determined by a luciferase reporter gene assay. The expressions of phosphorylated stat3, bcl-2, survivin, and caspase-3 were examined by Western blot analysis. AgNPs showed dose-dependent cytotoxicity and stimulation of apoptosis in H1299 cells. The effects on H1299 cells correlated well with the inhibition of NF-κB activity, a decrease in bcl-2, and an increase in caspase-3 and survivin expression. AgNPs significantly suppressed the H1299 tumor growth in a xenograft severe combined immunodeficient (SCID) mouse model. The results demonstrate the anticancer activities of AgNPs, suggesting that they may act as potential beneficial molecules in lung cancer chemoprevention and chemotherapy, especially for early-stage intervention. Keywords: silver nanoparticles, antitumor, lung cancer, cytotoxicity, H1299
format	article
author	He Y Du Z Ma S Liu Y Li D Huang H Jiang S Cheng S Wu W Zhang K Zheng X
author_facet	He Y Du Z Ma S Liu Y Li D Huang H Jiang S Cheng S Wu W Zhang K Zheng X
author_sort	He Y
title	Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo
title_short	Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo
title_full	Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo
title_fullStr	Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo
title_full_unstemmed	Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo
title_sort	effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/a08bdcf9c65c49bc922b23981b5c083b
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Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo

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