Differential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae.

Canola (oilseed rape, Brassica napus L.) is susceptible to infection by the biotrophic protist Plasmodiophora brassicae, the causal agent of clubroot. To understand the roles of microRNAs (miRNAs) during the post-transcriptional regulation of disease initiation and progression, we have characterized...

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Autores principales:	Shiv S Verma, Muhammad H Rahman, Michael K Deyholos, Urmila Basu, Nat N V Kav
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a08cebcff8e34c788e2100fb59cfa720
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spelling	oai:doaj.org-article:a08cebcff8e34c788e2100fb59cfa7202021-11-18T08:34:41ZDifferential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae.1932-620310.1371/journal.pone.0086648https://doaj.org/article/a08cebcff8e34c788e2100fb59cfa7202014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24497962/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Canola (oilseed rape, Brassica napus L.) is susceptible to infection by the biotrophic protist Plasmodiophora brassicae, the causal agent of clubroot. To understand the roles of microRNAs (miRNAs) during the post-transcriptional regulation of disease initiation and progression, we have characterized the changes in miRNA expression profiles in canola roots during clubroot disease development and have compared these to uninfected roots. Two different stages of clubroot development were targeted in this miRNA profiling study: an early time of 10-dpi for disease initiation and a later 20-dpi, by which time the pathogen had colonized the roots (as evident by visible gall formation and histological observations). P. brassicae responsive miRNAs were identified and validated by qRT-PCR of miRNAs and the subsequent validation of the target mRNAs through starBase degradome analysis, and through 5' RLM-RACE. This study identifies putative miRNA-regulated genes with roles during clubroot disease initiation and development. Putative target genes identified in this study included: transcription factors (TFs), hormone-related genes, as well as genes associated with plant stress response regulation such as cytokinin, auxin/ethylene response elements. The results of our study may assist in elucidating the role of miRNAs in post-transcriptional regulation of target genes during disease development and may contribute to the development of strategies to engineer durable resistance to this important phytopathogen.Shiv S VermaMuhammad H RahmanMichael K DeyholosUrmila BasuNat N V KavPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86648 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shiv S Verma Muhammad H Rahman Michael K Deyholos Urmila Basu Nat N V Kav Differential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae.
description	Canola (oilseed rape, Brassica napus L.) is susceptible to infection by the biotrophic protist Plasmodiophora brassicae, the causal agent of clubroot. To understand the roles of microRNAs (miRNAs) during the post-transcriptional regulation of disease initiation and progression, we have characterized the changes in miRNA expression profiles in canola roots during clubroot disease development and have compared these to uninfected roots. Two different stages of clubroot development were targeted in this miRNA profiling study: an early time of 10-dpi for disease initiation and a later 20-dpi, by which time the pathogen had colonized the roots (as evident by visible gall formation and histological observations). P. brassicae responsive miRNAs were identified and validated by qRT-PCR of miRNAs and the subsequent validation of the target mRNAs through starBase degradome analysis, and through 5' RLM-RACE. This study identifies putative miRNA-regulated genes with roles during clubroot disease initiation and development. Putative target genes identified in this study included: transcription factors (TFs), hormone-related genes, as well as genes associated with plant stress response regulation such as cytokinin, auxin/ethylene response elements. The results of our study may assist in elucidating the role of miRNAs in post-transcriptional regulation of target genes during disease development and may contribute to the development of strategies to engineer durable resistance to this important phytopathogen.
format	article
author	Shiv S Verma Muhammad H Rahman Michael K Deyholos Urmila Basu Nat N V Kav
author_facet	Shiv S Verma Muhammad H Rahman Michael K Deyholos Urmila Basu Nat N V Kav
author_sort	Shiv S Verma
title	Differential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae.
title_short	Differential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae.
title_full	Differential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae.
title_fullStr	Differential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae.
title_full_unstemmed	Differential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae.
title_sort	differential expression of mirnas in brassica napus root following infection with plasmodiophora brassicae.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/a08cebcff8e34c788e2100fb59cfa720
work_keys_str_mv	AT shivsverma differentialexpressionofmirnasinbrassicanapusrootfollowinginfectionwithplasmodiophorabrassicae AT muhammadhrahman differentialexpressionofmirnasinbrassicanapusrootfollowinginfectionwithplasmodiophorabrassicae AT michaelkdeyholos differentialexpressionofmirnasinbrassicanapusrootfollowinginfectionwithplasmodiophorabrassicae AT urmilabasu differentialexpressionofmirnasinbrassicanapusrootfollowinginfectionwithplasmodiophorabrassicae AT natnvkav differentialexpressionofmirnasinbrassicanapusrootfollowinginfectionwithplasmodiophorabrassicae
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Differential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae.

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