High humidity aggravates the severity of arthritis in collagen-induced arthritis mice by upregulating xylitol and L-pyroglutamic acid

Abstract Background Humidity was an unfavorable factor for patients with rheumatoid arthritis (RA). RA disease activity was severe in high humidity conditions. However, there is no evidence to demonstrate the effects of humidity on arthritis in the animal experiments and explore its relevant mechani...

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Autores principales:	Mingzhu Wang, Jiao Chen, Xiaoying Lin, Lin Huang, Haichang Li, Chengping Wen, Zhixing He
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Rheumatoid arthritis Humidity Collagen-induced arthritis Xylitol L-pyroglutamic acid DBA/1 mice Diseases of the musculoskeletal system RC925-935
Acceso en línea:	https://doaj.org/article/a0917a7afca945ccabba009efd92563e
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spelling	oai:doaj.org-article:a0917a7afca945ccabba009efd92563e2021-12-05T12:04:47ZHigh humidity aggravates the severity of arthritis in collagen-induced arthritis mice by upregulating xylitol and L-pyroglutamic acid10.1186/s13075-021-02681-x1478-6362https://doaj.org/article/a0917a7afca945ccabba009efd92563e2021-12-01T00:00:00Zhttps://doi.org/10.1186/s13075-021-02681-xhttps://doaj.org/toc/1478-6362Abstract Background Humidity was an unfavorable factor for patients with rheumatoid arthritis (RA). RA disease activity was severe in high humidity conditions. However, there is no evidence to demonstrate the effects of humidity on arthritis in the animal experiments and explore its relevant mechanism. Methods Using the DBA/1 mice, this study addressed the effects of a high humidity (80 ± 5%) on arthritis in collagen-induced arthritis (CIA) mice. Then, this study used the gas chromatography-mass spectrometer (GC-MS) to explore alterations in serum metabolome caused by the high humidity. Furthermore, xylitol and L-pyroglutamic acid, which were both significantly upregulated by the high humidity, were selected to further study their effects on arthritis in the CIA mice. Results The high humidity (80 ± 5%) could aggravate arthritis variables including increasing arthritis score and swelling, serum autoantibodies (anti-COII and anti-CCP), and proinflammatory cytokines (IL-6, IL-17A, and G-CSF). In addition, the high humidity could cause significant alterations in serum metabolome in the CIA mice. Xylitol and L-pyroglutamic acid were the representative serum metabolites that were significantly upregulated by the high humidity. Further experiments demonstrated that the supplementation of 0.4 mg/mL xylitol in drinking water after inducing the CIA model and 2.0 mg/mL in drinking water before inducing the CIA model could both aggravate arthritis in the CIA mice. Conclusions These data demonstrated that high humidity was not beneficial for arthritis development and its mechanism might be associated with xylitol and L-pyroglutamic acid.Mingzhu WangJiao ChenXiaoying LinLin HuangHaichang LiChengping WenZhixing HeBMCarticleRheumatoid arthritisHumidityCollagen-induced arthritisXylitolL-pyroglutamic acidDBA/1 miceDiseases of the musculoskeletal systemRC925-935ENArthritis Research & Therapy, Vol 23, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Rheumatoid arthritis Humidity Collagen-induced arthritis Xylitol L-pyroglutamic acid DBA/1 mice Diseases of the musculoskeletal system RC925-935
spellingShingle	Rheumatoid arthritis Humidity Collagen-induced arthritis Xylitol L-pyroglutamic acid DBA/1 mice Diseases of the musculoskeletal system RC925-935 Mingzhu Wang Jiao Chen Xiaoying Lin Lin Huang Haichang Li Chengping Wen Zhixing He High humidity aggravates the severity of arthritis in collagen-induced arthritis mice by upregulating xylitol and L-pyroglutamic acid
description	Abstract Background Humidity was an unfavorable factor for patients with rheumatoid arthritis (RA). RA disease activity was severe in high humidity conditions. However, there is no evidence to demonstrate the effects of humidity on arthritis in the animal experiments and explore its relevant mechanism. Methods Using the DBA/1 mice, this study addressed the effects of a high humidity (80 ± 5%) on arthritis in collagen-induced arthritis (CIA) mice. Then, this study used the gas chromatography-mass spectrometer (GC-MS) to explore alterations in serum metabolome caused by the high humidity. Furthermore, xylitol and L-pyroglutamic acid, which were both significantly upregulated by the high humidity, were selected to further study their effects on arthritis in the CIA mice. Results The high humidity (80 ± 5%) could aggravate arthritis variables including increasing arthritis score and swelling, serum autoantibodies (anti-COII and anti-CCP), and proinflammatory cytokines (IL-6, IL-17A, and G-CSF). In addition, the high humidity could cause significant alterations in serum metabolome in the CIA mice. Xylitol and L-pyroglutamic acid were the representative serum metabolites that were significantly upregulated by the high humidity. Further experiments demonstrated that the supplementation of 0.4 mg/mL xylitol in drinking water after inducing the CIA model and 2.0 mg/mL in drinking water before inducing the CIA model could both aggravate arthritis in the CIA mice. Conclusions These data demonstrated that high humidity was not beneficial for arthritis development and its mechanism might be associated with xylitol and L-pyroglutamic acid.
format	article
author	Mingzhu Wang Jiao Chen Xiaoying Lin Lin Huang Haichang Li Chengping Wen Zhixing He
author_facet	Mingzhu Wang Jiao Chen Xiaoying Lin Lin Huang Haichang Li Chengping Wen Zhixing He
author_sort	Mingzhu Wang
title	High humidity aggravates the severity of arthritis in collagen-induced arthritis mice by upregulating xylitol and L-pyroglutamic acid
title_short	High humidity aggravates the severity of arthritis in collagen-induced arthritis mice by upregulating xylitol and L-pyroglutamic acid
title_full	High humidity aggravates the severity of arthritis in collagen-induced arthritis mice by upregulating xylitol and L-pyroglutamic acid
title_fullStr	High humidity aggravates the severity of arthritis in collagen-induced arthritis mice by upregulating xylitol and L-pyroglutamic acid
title_full_unstemmed	High humidity aggravates the severity of arthritis in collagen-induced arthritis mice by upregulating xylitol and L-pyroglutamic acid
title_sort	high humidity aggravates the severity of arthritis in collagen-induced arthritis mice by upregulating xylitol and l-pyroglutamic acid
publisher	BMC
publishDate	2021
url	https://doaj.org/article/a0917a7afca945ccabba009efd92563e
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High humidity aggravates the severity of arthritis in collagen-induced arthritis mice by upregulating xylitol and L-pyroglutamic acid

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