Transcriptome profiling reveals potential genes involved in browning of fresh-cut eggplant (Solanum melongena L.)

Abstract Fresh-cut processing promotes enzymatic browning of fresh fruits and vegetables, which negatively affects the product appearance and impacts their nutrition. We used RNA-sequencing to analyze the transcriptomic changes occurring during the browning of fresh-cut eggplant fruit samples from b...

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Autores principales:	Xiaohui Liu, Aidong Zhang, Jie Zhao, Jing Shang, Zongwen Zhu, Xuexia Wu, Dingshi Zha
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a0971fcc1ca94b6daafcbfc0d15e0c7b
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spelling	oai:doaj.org-article:a0971fcc1ca94b6daafcbfc0d15e0c7b2021-12-02T15:07:54ZTranscriptome profiling reveals potential genes involved in browning of fresh-cut eggplant (Solanum melongena L.)10.1038/s41598-021-94831-z2045-2322https://doaj.org/article/a0971fcc1ca94b6daafcbfc0d15e0c7b2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94831-zhttps://doaj.org/toc/2045-2322Abstract Fresh-cut processing promotes enzymatic browning of fresh fruits and vegetables, which negatively affects the product appearance and impacts their nutrition. We used RNA-sequencing to analyze the transcriptomic changes occurring during the browning of fresh-cut eggplant fruit samples from both browning-sensitive and browning-resistant cultivars to investigate the molecular mechanisms involved in browning. A total of 8347 differentially expressed genes were identified, of which 62 genes were from six gene families (i.e., PPO, PAL, POD, CAT, APX, and GST) potentially associated with enzymatic browning. Furthermore, using qRT-PCR, we verified 231 differentially regulated transcription factors in fresh-cut eggplant fruits. The enzyme activities of PPO, POD, PAL, and CAT in ‘36’ were significantly higher than those of ‘F’ fresh-cut for 15 min. Both PPO and POD play a major role in the browning of eggplant pulp and might therefore act synergistically in the browning process. Meanwhile, qPCR results of 18 browning related genes randomly screened in 15 eggplant materials with different browning tolerance showed variant-specific expression of genes. Lastly, gene regulatory networks were constructed to identify the browning-related genes. This work provides a basis for future molecular studies of eggplants, and lays a theoretical foundation for the development of browning-resistant fresh-cut fruits and vegetables.Xiaohui LiuAidong ZhangJie ZhaoJing ShangZongwen ZhuXuexia WuDingshi ZhaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaohui Liu Aidong Zhang Jie Zhao Jing Shang Zongwen Zhu Xuexia Wu Dingshi Zha Transcriptome profiling reveals potential genes involved in browning of fresh-cut eggplant (Solanum melongena L.)
description	Abstract Fresh-cut processing promotes enzymatic browning of fresh fruits and vegetables, which negatively affects the product appearance and impacts their nutrition. We used RNA-sequencing to analyze the transcriptomic changes occurring during the browning of fresh-cut eggplant fruit samples from both browning-sensitive and browning-resistant cultivars to investigate the molecular mechanisms involved in browning. A total of 8347 differentially expressed genes were identified, of which 62 genes were from six gene families (i.e., PPO, PAL, POD, CAT, APX, and GST) potentially associated with enzymatic browning. Furthermore, using qRT-PCR, we verified 231 differentially regulated transcription factors in fresh-cut eggplant fruits. The enzyme activities of PPO, POD, PAL, and CAT in ‘36’ were significantly higher than those of ‘F’ fresh-cut for 15 min. Both PPO and POD play a major role in the browning of eggplant pulp and might therefore act synergistically in the browning process. Meanwhile, qPCR results of 18 browning related genes randomly screened in 15 eggplant materials with different browning tolerance showed variant-specific expression of genes. Lastly, gene regulatory networks were constructed to identify the browning-related genes. This work provides a basis for future molecular studies of eggplants, and lays a theoretical foundation for the development of browning-resistant fresh-cut fruits and vegetables.
format	article
author	Xiaohui Liu Aidong Zhang Jie Zhao Jing Shang Zongwen Zhu Xuexia Wu Dingshi Zha
author_facet	Xiaohui Liu Aidong Zhang Jie Zhao Jing Shang Zongwen Zhu Xuexia Wu Dingshi Zha
author_sort	Xiaohui Liu
title	Transcriptome profiling reveals potential genes involved in browning of fresh-cut eggplant (Solanum melongena L.)
title_short	Transcriptome profiling reveals potential genes involved in browning of fresh-cut eggplant (Solanum melongena L.)
title_full	Transcriptome profiling reveals potential genes involved in browning of fresh-cut eggplant (Solanum melongena L.)
title_fullStr	Transcriptome profiling reveals potential genes involved in browning of fresh-cut eggplant (Solanum melongena L.)
title_full_unstemmed	Transcriptome profiling reveals potential genes involved in browning of fresh-cut eggplant (Solanum melongena L.)
title_sort	transcriptome profiling reveals potential genes involved in browning of fresh-cut eggplant (solanum melongena l.)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a0971fcc1ca94b6daafcbfc0d15e0c7b
work_keys_str_mv	AT xiaohuiliu transcriptomeprofilingrevealspotentialgenesinvolvedinbrowningoffreshcuteggplantsolanummelongenal AT aidongzhang transcriptomeprofilingrevealspotentialgenesinvolvedinbrowningoffreshcuteggplantsolanummelongenal AT jiezhao transcriptomeprofilingrevealspotentialgenesinvolvedinbrowningoffreshcuteggplantsolanummelongenal AT jingshang transcriptomeprofilingrevealspotentialgenesinvolvedinbrowningoffreshcuteggplantsolanummelongenal AT zongwenzhu transcriptomeprofilingrevealspotentialgenesinvolvedinbrowningoffreshcuteggplantsolanummelongenal AT xuexiawu transcriptomeprofilingrevealspotentialgenesinvolvedinbrowningoffreshcuteggplantsolanummelongenal AT dingshizha transcriptomeprofilingrevealspotentialgenesinvolvedinbrowningoffreshcuteggplantsolanummelongenal
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Transcriptome profiling reveals potential genes involved in browning of fresh-cut eggplant (Solanum melongena L.)

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