Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering

Existing in vivo mutagenesis tools are limited by low mutation diversity and mutation rates. Here the authors present TRIDENT for targeted, continual and inducible diversification of genes of interest using deaminases fused to T7 RNA polymerase.

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Detalles Bibliográficos
Autores principales: Aaron Cravens, Osman K. Jamil, Deze Kong, Jonathan T. Sockolosky, Christina D. Smolke
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/a09839f69fe344c5b0b778bd2bc12f32
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Descripción
Sumario:Existing in vivo mutagenesis tools are limited by low mutation diversity and mutation rates. Here the authors present TRIDENT for targeted, continual and inducible diversification of genes of interest using deaminases fused to T7 RNA polymerase.