Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering

Existing in vivo mutagenesis tools are limited by low mutation diversity and mutation rates. Here the authors present TRIDENT for targeted, continual and inducible diversification of genes of interest using deaminases fused to T7 RNA polymerase.

Guardado en:

Detalles Bibliográficos
Autores principales:	Aaron Cravens, Osman K. Jamil, Deze Kong, Jonathan T. Sockolosky, Christina D. Smolke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a09839f69fe344c5b0b778bd2bc12f32
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a09839f69fe344c5b0b778bd2bc12f32
record_format	dspace
spelling	oai:doaj.org-article:a09839f69fe344c5b0b778bd2bc12f322021-12-02T13:32:54ZPolymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering10.1038/s41467-021-21876-z2041-1723https://doaj.org/article/a09839f69fe344c5b0b778bd2bc12f322021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21876-zhttps://doaj.org/toc/2041-1723Existing in vivo mutagenesis tools are limited by low mutation diversity and mutation rates. Here the authors present TRIDENT for targeted, continual and inducible diversification of genes of interest using deaminases fused to T7 RNA polymerase.Aaron CravensOsman K. JamilDeze KongJonathan T. SockoloskyChristina D. SmolkeNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Aaron Cravens Osman K. Jamil Deze Kong Jonathan T. Sockolosky Christina D. Smolke Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering
description	Existing in vivo mutagenesis tools are limited by low mutation diversity and mutation rates. Here the authors present TRIDENT for targeted, continual and inducible diversification of genes of interest using deaminases fused to T7 RNA polymerase.
format	article
author	Aaron Cravens Osman K. Jamil Deze Kong Jonathan T. Sockolosky Christina D. Smolke
author_facet	Aaron Cravens Osman K. Jamil Deze Kong Jonathan T. Sockolosky Christina D. Smolke
author_sort	Aaron Cravens
title	Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering
title_short	Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering
title_full	Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering
title_fullStr	Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering
title_full_unstemmed	Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering
title_sort	polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a09839f69fe344c5b0b778bd2bc12f32
work_keys_str_mv	AT aaroncravens polymeraseguidedbaseeditingenablesinvivomutagenesisandrapidproteinengineering AT osmankjamil polymeraseguidedbaseeditingenablesinvivomutagenesisandrapidproteinengineering AT dezekong polymeraseguidedbaseeditingenablesinvivomutagenesisandrapidproteinengineering AT jonathantsockolosky polymeraseguidedbaseeditingenablesinvivomutagenesisandrapidproteinengineering AT christinadsmolke polymeraseguidedbaseeditingenablesinvivomutagenesisandrapidproteinengineering
_version_	1718392871160119296

Polymerase-guided base editing enables in vivo mutagenesis and rapid protein engineering

Ejemplares similares