Transcriptomic Analysis of Laying Hens Revealed the Role of Aging-Related Genes during Forced Molting
Molting in birds provides us with an ideal genetic model for understanding aging and rejuvenation since birds present younger characteristics for reproduction and appearance after molting. Forced molting (FM) by fasting in chickens causes aging of their reproductive system and then promotes cell red...
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2021
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oai:doaj.org-article:a0a174b8a6d14c678224bedf695a31dc2021-11-25T17:41:42ZTranscriptomic Analysis of Laying Hens Revealed the Role of Aging-Related Genes during Forced Molting10.3390/genes121117672073-4425https://doaj.org/article/a0a174b8a6d14c678224bedf695a31dc2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1767https://doaj.org/toc/2073-4425Molting in birds provides us with an ideal genetic model for understanding aging and rejuvenation since birds present younger characteristics for reproduction and appearance after molting. Forced molting (FM) by fasting in chickens causes aging of their reproductive system and then promotes cell redevelopment by providing water and feed again. To reveal the genetic mechanism of rejuvenation, we detected blood hormone indexes and gene expression levels in the hypothalamus and ovary of hens from five different periods during FM. Three hormones were identified as participating in FM. Furthermore, the variation trends of gene expression levels in the hypothalamus and ovary at five different stages were found to be basically similar using transcriptome analysis. Among them, 45 genes were found to regulate cell aging during fasting stress and 12 genes were found to promote cell development during the recovery period in the hypothalamus. In addition, five hub genes (<i>INO80D, HELZ, AGO4, ROCK2</i><i>,</i> and <i>RFX7</i>) were identified by WGCNA. FM can restart the reproductive function of aged hens by regulating expression levels of genes associated with aging and development. Our study not only enriches the theoretical basis of FM but also provides insights for the study of antiaging in humans and the conception mechanism in elderly women.Tongyu ZhangYu ChenJunhui WenYaxiong JiaLiang WangXueze LvWeifang YangChangqing QuHaiying LiHuie WangLujiang QuZhonghua NingMDPI AGarticleforced moltingagingrejuvenationtranscriptome analysishensGeneticsQH426-470ENGenes, Vol 12, Iss 1767, p 1767 (2021) |
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forced molting aging rejuvenation transcriptome analysis hens Genetics QH426-470 |
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forced molting aging rejuvenation transcriptome analysis hens Genetics QH426-470 Tongyu Zhang Yu Chen Junhui Wen Yaxiong Jia Liang Wang Xueze Lv Weifang Yang Changqing Qu Haiying Li Huie Wang Lujiang Qu Zhonghua Ning Transcriptomic Analysis of Laying Hens Revealed the Role of Aging-Related Genes during Forced Molting |
| description |
Molting in birds provides us with an ideal genetic model for understanding aging and rejuvenation since birds present younger characteristics for reproduction and appearance after molting. Forced molting (FM) by fasting in chickens causes aging of their reproductive system and then promotes cell redevelopment by providing water and feed again. To reveal the genetic mechanism of rejuvenation, we detected blood hormone indexes and gene expression levels in the hypothalamus and ovary of hens from five different periods during FM. Three hormones were identified as participating in FM. Furthermore, the variation trends of gene expression levels in the hypothalamus and ovary at five different stages were found to be basically similar using transcriptome analysis. Among them, 45 genes were found to regulate cell aging during fasting stress and 12 genes were found to promote cell development during the recovery period in the hypothalamus. In addition, five hub genes (<i>INO80D, HELZ, AGO4, ROCK2</i><i>,</i> and <i>RFX7</i>) were identified by WGCNA. FM can restart the reproductive function of aged hens by regulating expression levels of genes associated with aging and development. Our study not only enriches the theoretical basis of FM but also provides insights for the study of antiaging in humans and the conception mechanism in elderly women. |
| format |
article |
| author |
Tongyu Zhang Yu Chen Junhui Wen Yaxiong Jia Liang Wang Xueze Lv Weifang Yang Changqing Qu Haiying Li Huie Wang Lujiang Qu Zhonghua Ning |
| author_facet |
Tongyu Zhang Yu Chen Junhui Wen Yaxiong Jia Liang Wang Xueze Lv Weifang Yang Changqing Qu Haiying Li Huie Wang Lujiang Qu Zhonghua Ning |
| author_sort |
Tongyu Zhang |
| title |
Transcriptomic Analysis of Laying Hens Revealed the Role of Aging-Related Genes during Forced Molting |
| title_short |
Transcriptomic Analysis of Laying Hens Revealed the Role of Aging-Related Genes during Forced Molting |
| title_full |
Transcriptomic Analysis of Laying Hens Revealed the Role of Aging-Related Genes during Forced Molting |
| title_fullStr |
Transcriptomic Analysis of Laying Hens Revealed the Role of Aging-Related Genes during Forced Molting |
| title_full_unstemmed |
Transcriptomic Analysis of Laying Hens Revealed the Role of Aging-Related Genes during Forced Molting |
| title_sort |
transcriptomic analysis of laying hens revealed the role of aging-related genes during forced molting |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/a0a174b8a6d14c678224bedf695a31dc |
| work_keys_str_mv |
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| _version_ |
1718412120369922048 |