Evaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells

Abstract Moxifloxacin hydrochloride (MXF) is widely used for the prevention of bacterial endophthalmitis after intraocular surgeries. However, the safety issue of intracameral injection of MXF for human corneal endothelial cells (HCECs) is still debatable. In this study, we investigated concentratio...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Joo-Hee Park, Martha Kim, Roy S. Chuck, Choul Yong Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a0ac426df62a4eb6b968e203eee7b507
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a0ac426df62a4eb6b968e203eee7b507
record_format	dspace
spelling	oai:doaj.org-article:a0ac426df62a4eb6b968e203eee7b5072021-12-02T11:39:20ZEvaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells10.1038/s41598-021-85834-x2045-2322https://doaj.org/article/a0ac426df62a4eb6b968e203eee7b5072021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85834-xhttps://doaj.org/toc/2045-2322Abstract Moxifloxacin hydrochloride (MXF) is widely used for the prevention of bacterial endophthalmitis after intraocular surgeries. However, the safety issue of intracameral injection of MXF for human corneal endothelial cells (HCECs) is still debatable. In this study, we investigated concentration-dependent cytotoxicity (0.05–1 mg/ml) of MXF for immortalized HCECs (B4G12 cell) and the underlying mechanism. Reactive oxygen generation (ROS) and cell viability after MXF exposure was measured. Flow cytometric analysis and TUNEL assay was used to detect apoptotic HCECs after MXF exposure. Ultrastructure of damaged HCECs by MXF was imaged by transmission electron microscope. Western blot analysis and caspase 2, 3 and 8 analysis were used to reveal the underlying mechanism of MXF induced damage in HCECs. We found that MXF induced dose-dependent cytotoxicity in HCECs. MXF exposure increased ROS generation and induced autophagy in HCECs. Increased LDH release represented the cellular membrane damage by MXF. In addition, caspases activation, Bax/Bcl-xL-dependent apoptosis pathway and apoptosis inducing factor nuclear translocation were all involved in MXF induced HCECs’ damage, especially after exposure to high dose of MXF (0.5 and 1.0 mg/ml). These findings suggest that MXF toxicity on HCECs should be thoroughly considered by ophthalmologists when intracameral injection of MXF is planned.Joo-Hee ParkMartha KimRoy S. ChuckChoul Yong ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joo-Hee Park Martha Kim Roy S. Chuck Choul Yong Park Evaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells
description	Abstract Moxifloxacin hydrochloride (MXF) is widely used for the prevention of bacterial endophthalmitis after intraocular surgeries. However, the safety issue of intracameral injection of MXF for human corneal endothelial cells (HCECs) is still debatable. In this study, we investigated concentration-dependent cytotoxicity (0.05–1 mg/ml) of MXF for immortalized HCECs (B4G12 cell) and the underlying mechanism. Reactive oxygen generation (ROS) and cell viability after MXF exposure was measured. Flow cytometric analysis and TUNEL assay was used to detect apoptotic HCECs after MXF exposure. Ultrastructure of damaged HCECs by MXF was imaged by transmission electron microscope. Western blot analysis and caspase 2, 3 and 8 analysis were used to reveal the underlying mechanism of MXF induced damage in HCECs. We found that MXF induced dose-dependent cytotoxicity in HCECs. MXF exposure increased ROS generation and induced autophagy in HCECs. Increased LDH release represented the cellular membrane damage by MXF. In addition, caspases activation, Bax/Bcl-xL-dependent apoptosis pathway and apoptosis inducing factor nuclear translocation were all involved in MXF induced HCECs’ damage, especially after exposure to high dose of MXF (0.5 and 1.0 mg/ml). These findings suggest that MXF toxicity on HCECs should be thoroughly considered by ophthalmologists when intracameral injection of MXF is planned.
format	article
author	Joo-Hee Park Martha Kim Roy S. Chuck Choul Yong Park
author_facet	Joo-Hee Park Martha Kim Roy S. Chuck Choul Yong Park
author_sort	Joo-Hee Park
title	Evaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells
title_short	Evaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells
title_full	Evaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells
title_fullStr	Evaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells
title_full_unstemmed	Evaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells
title_sort	evaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a0ac426df62a4eb6b968e203eee7b507
work_keys_str_mv	AT jooheepark evaluationofmoxifloxacininducedcytotoxicityonhumancornealendothelialcells AT marthakim evaluationofmoxifloxacininducedcytotoxicityonhumancornealendothelialcells AT royschuck evaluationofmoxifloxacininducedcytotoxicityonhumancornealendothelialcells AT choulyongpark evaluationofmoxifloxacininducedcytotoxicityonhumancornealendothelialcells
_version_	1718395716733239296

Evaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells

Ejemplares similares