Transcriptome analysis of MSC and MSC-derived osteoblasts on Resomer® LT706 and PCL: impact of biomaterial substrate on osteogenic differentiation.

<h4>Background</h4>Mesenchymal stem cells (MSC) represent a particularly attractive cell type for bone tissue engineering because of their ex vivo expansion potential and multipotent differentiation capacity. MSC are readily differentiated towards mature osteoblasts with well-established...

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Autores principales: Sabine Neuss, Bernd Denecke, Lin Gan, Qiong Lin, Manfred Bovi, Christian Apel, Michael Wöltje, Anandhan Dhanasingh, Jochen Salber, Ruth Knüchel, Martin Zenke
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spelling oai:doaj.org-article:a0ac437f68d94600adc954e361afdeda2021-11-04T06:08:40ZTranscriptome analysis of MSC and MSC-derived osteoblasts on Resomer® LT706 and PCL: impact of biomaterial substrate on osteogenic differentiation.1932-620310.1371/journal.pone.0023195https://doaj.org/article/a0ac437f68d94600adc954e361afdeda2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21935359/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Mesenchymal stem cells (MSC) represent a particularly attractive cell type for bone tissue engineering because of their ex vivo expansion potential and multipotent differentiation capacity. MSC are readily differentiated towards mature osteoblasts with well-established protocols. However, tissue engineering frequently involves three-dimensional scaffolds which (i) allow for cell adhesion in a spatial environment and (ii) meet application-specific criteria, such as stiffness, degradability and biocompatibility.<h4>Methodology/principal findings</h4>In the present study, we analysed two synthetic, long-term degradable polymers for their impact on MSC-based bone tissue engineering: PLLA-co-TMC (Resomer® LT706) and poly(ε-caprolactone) (PCL). Both polymers enhance the osteogenic differentiation compared to tissue culture polystyrene (TCPS) as determined by Alizarin red stainings, scanning electron microscopy, PCR and whole genome expression analysis. Resomer® LT706 and PCL differ in their influence on gene expression, with Resomer® LT706 being more potent in supporting osteogenic differentiation of MSC. The major trigger on the osteogenic fate, however, is from osteogenic induction medium.<h4>Conclusion</h4>This study demonstrates an enhanced osteogenic differentiation of MSC on Resomer® LT706 and PCL compared to TCPS. MSC cultured on Resomer® LT706 showed higher numbers of genes involved in skeletal development and bone formation. This identifies Resomer® LT706 as particularly attractive scaffold material for bone tissue engineering.Sabine NeussBernd DeneckeLin GanQiong LinManfred BoviChristian ApelMichael WöltjeAnandhan DhanasinghJochen SalberRuth KnüchelMartin ZenkePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e23195 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Sabine Neuss
Bernd Denecke
Lin Gan
Qiong Lin
Manfred Bovi
Christian Apel
Michael Wöltje
Anandhan Dhanasingh
Jochen Salber
Ruth Knüchel
Martin Zenke
Transcriptome analysis of MSC and MSC-derived osteoblasts on Resomer® LT706 and PCL: impact of biomaterial substrate on osteogenic differentiation.
description <h4>Background</h4>Mesenchymal stem cells (MSC) represent a particularly attractive cell type for bone tissue engineering because of their ex vivo expansion potential and multipotent differentiation capacity. MSC are readily differentiated towards mature osteoblasts with well-established protocols. However, tissue engineering frequently involves three-dimensional scaffolds which (i) allow for cell adhesion in a spatial environment and (ii) meet application-specific criteria, such as stiffness, degradability and biocompatibility.<h4>Methodology/principal findings</h4>In the present study, we analysed two synthetic, long-term degradable polymers for their impact on MSC-based bone tissue engineering: PLLA-co-TMC (Resomer® LT706) and poly(ε-caprolactone) (PCL). Both polymers enhance the osteogenic differentiation compared to tissue culture polystyrene (TCPS) as determined by Alizarin red stainings, scanning electron microscopy, PCR and whole genome expression analysis. Resomer® LT706 and PCL differ in their influence on gene expression, with Resomer® LT706 being more potent in supporting osteogenic differentiation of MSC. The major trigger on the osteogenic fate, however, is from osteogenic induction medium.<h4>Conclusion</h4>This study demonstrates an enhanced osteogenic differentiation of MSC on Resomer® LT706 and PCL compared to TCPS. MSC cultured on Resomer® LT706 showed higher numbers of genes involved in skeletal development and bone formation. This identifies Resomer® LT706 as particularly attractive scaffold material for bone tissue engineering.
format article
author Sabine Neuss
Bernd Denecke
Lin Gan
Qiong Lin
Manfred Bovi
Christian Apel
Michael Wöltje
Anandhan Dhanasingh
Jochen Salber
Ruth Knüchel
Martin Zenke
author_facet Sabine Neuss
Bernd Denecke
Lin Gan
Qiong Lin
Manfred Bovi
Christian Apel
Michael Wöltje
Anandhan Dhanasingh
Jochen Salber
Ruth Knüchel
Martin Zenke
author_sort Sabine Neuss
title Transcriptome analysis of MSC and MSC-derived osteoblasts on Resomer® LT706 and PCL: impact of biomaterial substrate on osteogenic differentiation.
title_short Transcriptome analysis of MSC and MSC-derived osteoblasts on Resomer® LT706 and PCL: impact of biomaterial substrate on osteogenic differentiation.
title_full Transcriptome analysis of MSC and MSC-derived osteoblasts on Resomer® LT706 and PCL: impact of biomaterial substrate on osteogenic differentiation.
title_fullStr Transcriptome analysis of MSC and MSC-derived osteoblasts on Resomer® LT706 and PCL: impact of biomaterial substrate on osteogenic differentiation.
title_full_unstemmed Transcriptome analysis of MSC and MSC-derived osteoblasts on Resomer® LT706 and PCL: impact of biomaterial substrate on osteogenic differentiation.
title_sort transcriptome analysis of msc and msc-derived osteoblasts on resomer® lt706 and pcl: impact of biomaterial substrate on osteogenic differentiation.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/a0ac437f68d94600adc954e361afdeda
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