Hybrid Radiobioconjugated Superparamagnetic Iron Oxide-Based Nanoparticles for Multimodal Cancer Therapy

Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used for biomedical applications for their outstanding properties such as facile functionalization and doping with different metals, high surface-to-volume ratio, superparamagnetism, and biocompatibility. This study was designed to synth...

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Autores principales:	Michał Żuk, Weronika Gawęda, Agnieszka Majkowska-Pilip, Magdalena Osial, Marcin Wolski, Aleksander Bilewicz, Paweł Krysiński
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	SPION radio-labeled nanoparticles anticancer therapy magnetic hyperthermia drug delivery trastuzumab Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/a0b3b353f27143629d999b80b549b0e8
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spelling	oai:doaj.org-article:a0b3b353f27143629d999b80b549b0e82021-11-25T18:41:06ZHybrid Radiobioconjugated Superparamagnetic Iron Oxide-Based Nanoparticles for Multimodal Cancer Therapy10.3390/pharmaceutics131118431999-4923https://doaj.org/article/a0b3b353f27143629d999b80b549b0e82021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1843https://doaj.org/toc/1999-4923Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used for biomedical applications for their outstanding properties such as facile functionalization and doping with different metals, high surface-to-volume ratio, superparamagnetism, and biocompatibility. This study was designed to synthesize and investigate multifunctional nanoparticle conjugate to act as both a magnetic agent, anticancer immunological drug, and radiopharmaceutic for anticancer therapy. The carrier, <sup>166</sup>Ho doped iron oxide, was coated with an Au layer, creating core-shell nanoparticles ([<sup>166</sup>Ho] Fe<sub>3</sub>O<sub>4</sub>@Au. These nanoparticles were subsequently modified with monoclonal antibody trastuzumab (Tmab) to target HER2+ receptors. We describe the radiobioconjugate preparation involving doping of a radioactive agent and attachment of the organic linker and drug to the SPIONs’ surface. The size of the SPIONs coated with an Au shell measured by transmission electron microscopy was about 15 nm. The bioconjugation of trastuzumab onto SPIONs was confirmed by thermogravimetric analysis, and the amount of two molecules per one nanoparticle was estimated with the use of radioiodinated [<sup>131</sup>I]Tmab. The synthesized bioconjugates showed that they are efficient heat mediators and also exhibit a cytotoxic effect toward SKOV-3 ovarian cancer cells expressing HER2 receptors. Prepared radiobioconjugates reveal the high potential for in vivo application of the proposed multimodal hybrid system, combined with magnetic hyperthermia and immunotherapy against cancer tissues.Michał ŻukWeronika GawędaAgnieszka Majkowska-PilipMagdalena OsialMarcin WolskiAleksander BilewiczPaweł KrysińskiMDPI AGarticleSPIONradio-labeled nanoparticlesanticancer therapymagnetic hyperthermiadrug deliverytrastuzumabPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1843, p 1843 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SPION radio-labeled nanoparticles anticancer therapy magnetic hyperthermia drug delivery trastuzumab Pharmacy and materia medica RS1-441
spellingShingle	SPION radio-labeled nanoparticles anticancer therapy magnetic hyperthermia drug delivery trastuzumab Pharmacy and materia medica RS1-441 Michał Żuk Weronika Gawęda Agnieszka Majkowska-Pilip Magdalena Osial Marcin Wolski Aleksander Bilewicz Paweł Krysiński Hybrid Radiobioconjugated Superparamagnetic Iron Oxide-Based Nanoparticles for Multimodal Cancer Therapy
description	Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used for biomedical applications for their outstanding properties such as facile functionalization and doping with different metals, high surface-to-volume ratio, superparamagnetism, and biocompatibility. This study was designed to synthesize and investigate multifunctional nanoparticle conjugate to act as both a magnetic agent, anticancer immunological drug, and radiopharmaceutic for anticancer therapy. The carrier, <sup>166</sup>Ho doped iron oxide, was coated with an Au layer, creating core-shell nanoparticles ([<sup>166</sup>Ho] Fe<sub>3</sub>O<sub>4</sub>@Au. These nanoparticles were subsequently modified with monoclonal antibody trastuzumab (Tmab) to target HER2+ receptors. We describe the radiobioconjugate preparation involving doping of a radioactive agent and attachment of the organic linker and drug to the SPIONs’ surface. The size of the SPIONs coated with an Au shell measured by transmission electron microscopy was about 15 nm. The bioconjugation of trastuzumab onto SPIONs was confirmed by thermogravimetric analysis, and the amount of two molecules per one nanoparticle was estimated with the use of radioiodinated [<sup>131</sup>I]Tmab. The synthesized bioconjugates showed that they are efficient heat mediators and also exhibit a cytotoxic effect toward SKOV-3 ovarian cancer cells expressing HER2 receptors. Prepared radiobioconjugates reveal the high potential for in vivo application of the proposed multimodal hybrid system, combined with magnetic hyperthermia and immunotherapy against cancer tissues.
format	article
author	Michał Żuk Weronika Gawęda Agnieszka Majkowska-Pilip Magdalena Osial Marcin Wolski Aleksander Bilewicz Paweł Krysiński
author_facet	Michał Żuk Weronika Gawęda Agnieszka Majkowska-Pilip Magdalena Osial Marcin Wolski Aleksander Bilewicz Paweł Krysiński
author_sort	Michał Żuk
title	Hybrid Radiobioconjugated Superparamagnetic Iron Oxide-Based Nanoparticles for Multimodal Cancer Therapy
title_short	Hybrid Radiobioconjugated Superparamagnetic Iron Oxide-Based Nanoparticles for Multimodal Cancer Therapy
title_full	Hybrid Radiobioconjugated Superparamagnetic Iron Oxide-Based Nanoparticles for Multimodal Cancer Therapy
title_fullStr	Hybrid Radiobioconjugated Superparamagnetic Iron Oxide-Based Nanoparticles for Multimodal Cancer Therapy
title_full_unstemmed	Hybrid Radiobioconjugated Superparamagnetic Iron Oxide-Based Nanoparticles for Multimodal Cancer Therapy
title_sort	hybrid radiobioconjugated superparamagnetic iron oxide-based nanoparticles for multimodal cancer therapy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a0b3b353f27143629d999b80b549b0e8
work_keys_str_mv	AT michałzuk hybridradiobioconjugatedsuperparamagneticironoxidebasednanoparticlesformultimodalcancertherapy AT weronikagaweda hybridradiobioconjugatedsuperparamagneticironoxidebasednanoparticlesformultimodalcancertherapy AT agnieszkamajkowskapilip hybridradiobioconjugatedsuperparamagneticironoxidebasednanoparticlesformultimodalcancertherapy AT magdalenaosial hybridradiobioconjugatedsuperparamagneticironoxidebasednanoparticlesformultimodalcancertherapy AT marcinwolski hybridradiobioconjugatedsuperparamagneticironoxidebasednanoparticlesformultimodalcancertherapy AT aleksanderbilewicz hybridradiobioconjugatedsuperparamagneticironoxidebasednanoparticlesformultimodalcancertherapy AT pawełkrysinski hybridradiobioconjugatedsuperparamagneticironoxidebasednanoparticlesformultimodalcancertherapy
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Hybrid Radiobioconjugated Superparamagnetic Iron Oxide-Based Nanoparticles for Multimodal Cancer Therapy

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