Intracellular targeted co-delivery of shMDR1 and gefitinib with chitosan nanoparticles for overcoming multidrug resistance

Xiwei Yu,1,* Guang Yang,2,* Yijie Shi,1 Chang Su,3 Ming Liu,1 Bo Feng,1 Liang Zhao1 1School of Pharmacy, Liaoning Medical University, Jinzhou, People’s Republic of China; 2Department of Oncology, BenQ Medical Center, Nanjing Medical University, Nanjing, People’s Republic of Chin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yu XW, Yang G, Shi YJ, Su C, Liu M, Feng B, Zhao L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a0b94ca4acc64a8f90f8c73593f00820
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a0b94ca4acc64a8f90f8c73593f00820
record_format	dspace
spelling	oai:doaj.org-article:a0b94ca4acc64a8f90f8c73593f008202021-12-02T07:46:32ZIntracellular targeted co-delivery of shMDR1 and gefitinib with chitosan nanoparticles for overcoming multidrug resistance1178-2013https://doaj.org/article/a0b94ca4acc64a8f90f8c73593f008202015-11-01T00:00:00Zhttps://www.dovepress.com/intracellular-targeted-co-delivery-of-shmdr1-and-gefitinib-with-chitos-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xiwei Yu,1,* Guang Yang,2,* Yijie Shi,1 Chang Su,3 Ming Liu,1 Bo Feng,1 Liang Zhao1 1School of Pharmacy, Liaoning Medical University, Jinzhou, People’s Republic of China; 2Department of Oncology, BenQ Medical Center, Nanjing Medical University, Nanjing, People’s Republic of China; 3School of Veterinary Medicine, Liaoning Medical University, Jinzhou, People’s Republic of China *These authors contributed equally to this work Abstract: Nowadays, multidrug resistance and side effects of drugs limit the effectiveness of chemotherapies in clinics. P-glycoprotein (P-gp) (MDR1), as a member of the ATP-binding cassette family, acts on transporting drugs into cell plasma across the membrane of cancer cells and leads to the occurrence of multidrug resistance, thus resulting in the failure of chemotherapy in cancer. The main aims of this research were to design a nanodelivery system for accomplishing the effective co-delivery of gene and antitumor drug and overcoming multidrug resistance effect. In this study, shMDR1 and gefitinib-encapsulating chitosan nanoparticles with sustained release, small particle size, and high encapsulation efficiency were prepared. The serum stability, protection from nuclease, and transfection efficiency of gene in vitro were investigated. The effects of co-delivery of shMDR1 and gefitinib in nanoparticles on reversing multidrug resistance were also evaluated by investigating the cytotoxicity, cellular uptake mechanism, and cell apoptosis on established gefitinib-resistant cells. The results demonstrated that chitosan nanoparticles entrapping gefitinib and shMDR1 had the potential to overcome the multidrug resistance and improve cancer treatment efficacy, especially toward resistant cells. Keywords: P-glycoprotein, nanodelivery system, chemotherapy, geneYu XWYang GShi YJSu CLiu MFeng BZhao LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7045-7056 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yu XW Yang G Shi YJ Su C Liu M Feng B Zhao L Intracellular targeted co-delivery of shMDR1 and gefitinib with chitosan nanoparticles for overcoming multidrug resistance
description	Xiwei Yu,1,* Guang Yang,2,* Yijie Shi,1 Chang Su,3 Ming Liu,1 Bo Feng,1 Liang Zhao1 1School of Pharmacy, Liaoning Medical University, Jinzhou, People’s Republic of China; 2Department of Oncology, BenQ Medical Center, Nanjing Medical University, Nanjing, People’s Republic of China; 3School of Veterinary Medicine, Liaoning Medical University, Jinzhou, People’s Republic of China *These authors contributed equally to this work Abstract: Nowadays, multidrug resistance and side effects of drugs limit the effectiveness of chemotherapies in clinics. P-glycoprotein (P-gp) (MDR1), as a member of the ATP-binding cassette family, acts on transporting drugs into cell plasma across the membrane of cancer cells and leads to the occurrence of multidrug resistance, thus resulting in the failure of chemotherapy in cancer. The main aims of this research were to design a nanodelivery system for accomplishing the effective co-delivery of gene and antitumor drug and overcoming multidrug resistance effect. In this study, shMDR1 and gefitinib-encapsulating chitosan nanoparticles with sustained release, small particle size, and high encapsulation efficiency were prepared. The serum stability, protection from nuclease, and transfection efficiency of gene in vitro were investigated. The effects of co-delivery of shMDR1 and gefitinib in nanoparticles on reversing multidrug resistance were also evaluated by investigating the cytotoxicity, cellular uptake mechanism, and cell apoptosis on established gefitinib-resistant cells. The results demonstrated that chitosan nanoparticles entrapping gefitinib and shMDR1 had the potential to overcome the multidrug resistance and improve cancer treatment efficacy, especially toward resistant cells. Keywords: P-glycoprotein, nanodelivery system, chemotherapy, gene
format	article
author	Yu XW Yang G Shi YJ Su C Liu M Feng B Zhao L
author_facet	Yu XW Yang G Shi YJ Su C Liu M Feng B Zhao L
author_sort	Yu XW
title	Intracellular targeted co-delivery of shMDR1 and gefitinib with chitosan nanoparticles for overcoming multidrug resistance
title_short	Intracellular targeted co-delivery of shMDR1 and gefitinib with chitosan nanoparticles for overcoming multidrug resistance
title_full	Intracellular targeted co-delivery of shMDR1 and gefitinib with chitosan nanoparticles for overcoming multidrug resistance
title_fullStr	Intracellular targeted co-delivery of shMDR1 and gefitinib with chitosan nanoparticles for overcoming multidrug resistance
title_full_unstemmed	Intracellular targeted co-delivery of shMDR1 and gefitinib with chitosan nanoparticles for overcoming multidrug resistance
title_sort	intracellular targeted co-delivery of shmdr1 and gefitinib with chitosan nanoparticles for overcoming multidrug resistance
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/a0b94ca4acc64a8f90f8c73593f00820
work_keys_str_mv	AT yuxw intracellulartargetedcodeliveryofshmdr1andgefitinibwithchitosannanoparticlesforovercomingmultidrugresistance AT yangg intracellulartargetedcodeliveryofshmdr1andgefitinibwithchitosannanoparticlesforovercomingmultidrugresistance AT shiyj intracellulartargetedcodeliveryofshmdr1andgefitinibwithchitosannanoparticlesforovercomingmultidrugresistance AT suc intracellulartargetedcodeliveryofshmdr1andgefitinibwithchitosannanoparticlesforovercomingmultidrugresistance AT lium intracellulartargetedcodeliveryofshmdr1andgefitinibwithchitosannanoparticlesforovercomingmultidrugresistance AT fengb intracellulartargetedcodeliveryofshmdr1andgefitinibwithchitosannanoparticlesforovercomingmultidrugresistance AT zhaol intracellulartargetedcodeliveryofshmdr1andgefitinibwithchitosannanoparticlesforovercomingmultidrugresistance
_version_	1718399178351050752

Intracellular targeted co-delivery of shMDR1 and gefitinib with chitosan nanoparticles for overcoming multidrug resistance

Ejemplares similares