Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay

Cysticercosis is a neglected tropical disease caused by the larvae of Taenia solium in pigs and humans. The current diagnosis of porcine cysticercosis is difficult, and traditional pathological tests cannot meet the needs of detection. This study established a UPT-LF assay for the detection of Cysti...

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Autores principales:	Dejia Zhang, Yu Qi, Yaxuan Cui, Weiyi Song, Xinrui Wang, Mingyuan Liu, Xuepeng Cai, Xuenong Luo, Xiaolei Liu, Shumin Sun
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Taenia solium Cysticercus cellulosae antigen immune diagnosis UPT-LF Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a0b992c6d7864b6eb253c98bb5d310c7
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spelling	oai:doaj.org-article:a0b992c6d7864b6eb253c98bb5d310c72021-11-10T05:48:58ZRapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay2235-298810.3389/fcimb.2021.762472https://doaj.org/article/a0b992c6d7864b6eb253c98bb5d310c72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.762472/fullhttps://doaj.org/toc/2235-2988Cysticercosis is a neglected tropical disease caused by the larvae of Taenia solium in pigs and humans. The current diagnosis of porcine cysticercosis is difficult, and traditional pathological tests cannot meet the needs of detection. This study established a UPT-LF assay for the detection of Cysticercus cellulosae. UCP particles were bound to two antigens, TSOL18 and GP50; samples were captured, and the signal from the UCP particles was converted into a detectable signal for analysis using a biosensor. Compared to ELISA, UPT-LF has higher sensitivity and specificity, with a sensitivity of 93.59% and 97.44%, respectively, in the case of TSOL18 and GP50 antigens and a specificity of 100% for both. Given its rapidness, small volume, high sensitivity and specificity, and good stability and reproducibility, this method could be used in the diagnosis of cysticercosis.Dejia ZhangYu QiYaxuan CuiWeiyi SongXinrui WangMingyuan LiuXuepeng CaiXuenong LuoXiaolei LiuShumin SunShumin SunFrontiers Media S.A.articleTaenia soliumCysticercus cellulosaeantigenimmune diagnosisUPT-LFMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Taenia solium Cysticercus cellulosae antigen immune diagnosis UPT-LF Microbiology QR1-502
spellingShingle	Taenia solium Cysticercus cellulosae antigen immune diagnosis UPT-LF Microbiology QR1-502 Dejia Zhang Yu Qi Yaxuan Cui Weiyi Song Xinrui Wang Mingyuan Liu Xuepeng Cai Xuenong Luo Xiaolei Liu Shumin Sun Shumin Sun Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay
description	Cysticercosis is a neglected tropical disease caused by the larvae of Taenia solium in pigs and humans. The current diagnosis of porcine cysticercosis is difficult, and traditional pathological tests cannot meet the needs of detection. This study established a UPT-LF assay for the detection of Cysticercus cellulosae. UCP particles were bound to two antigens, TSOL18 and GP50; samples were captured, and the signal from the UCP particles was converted into a detectable signal for analysis using a biosensor. Compared to ELISA, UPT-LF has higher sensitivity and specificity, with a sensitivity of 93.59% and 97.44%, respectively, in the case of TSOL18 and GP50 antigens and a specificity of 100% for both. Given its rapidness, small volume, high sensitivity and specificity, and good stability and reproducibility, this method could be used in the diagnosis of cysticercosis.
format	article
author	Dejia Zhang Yu Qi Yaxuan Cui Weiyi Song Xinrui Wang Mingyuan Liu Xuepeng Cai Xuenong Luo Xiaolei Liu Shumin Sun Shumin Sun
author_facet	Dejia Zhang Yu Qi Yaxuan Cui Weiyi Song Xinrui Wang Mingyuan Liu Xuepeng Cai Xuenong Luo Xiaolei Liu Shumin Sun Shumin Sun
author_sort	Dejia Zhang
title	Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay
title_short	Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay
title_full	Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay
title_fullStr	Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay
title_full_unstemmed	Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay
title_sort	rapid detection of cysticercus cellulosae by an up-converting phosphor technology-based lateral-flow assay
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a0b992c6d7864b6eb253c98bb5d310c7
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Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay

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