Quantification of cytomegalovirus glycoprotein Bn DNA in hematopoietic stem cell transplant recipients by real-time PCR.

Based on sequence variation in the N-terminus of the UL55 gene, which encodes glycoprotein B (gB), human cytomegalovirus (CMV) can be classified into four gBn genotypes. We assessed the distribution of CMV gBn genotypes and the correlation between CMV gBn DNA (detected by real-time PCR) and CMV-posi...

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Autores principales:	Xuan Zhang, Ya Ping Huang, Hai Nv Gao, Mei Fang Yang, Hong Zhao, Jian Hua Hu, Xiao Ming Chen, Wei Hang Ma, Jun Fan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a0b9982ba40f4886b43afa8bc2d1ec11
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spelling	oai:doaj.org-article:a0b9982ba40f4886b43afa8bc2d1ec112021-11-18T08:05:46ZQuantification of cytomegalovirus glycoprotein Bn DNA in hematopoietic stem cell transplant recipients by real-time PCR.1932-620310.1371/journal.pone.0051224https://doaj.org/article/a0b9982ba40f4886b43afa8bc2d1ec112012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23251460/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Based on sequence variation in the N-terminus of the UL55 gene, which encodes glycoprotein B (gB), human cytomegalovirus (CMV) can be classified into four gBn genotypes. We assessed the distribution of CMV gBn genotypes and the correlation between CMV gBn DNA (detected by real-time PCR) and CMV-positive pp65 cells (identified by immunohistochemical staining) in a cohort of hematopoietic stem cell transplant patients. The distribution of gB genotypes was as follows: gBn1, 60% of patients; gBn2, 13.3%; mixed gBn1 and gBn3 infection, 26.7%; and gBn4 and other mixed infections, 0%. CMV gBn1 was the most common genotype. The detected level of CMV gB DNA correlated well with the number of CMV-positive pp65 cells detected by immunostaining (r = 0.585).Xuan ZhangYa Ping HuangHai Nv GaoMei Fang YangHong ZhaoJian Hua HuXiao Ming ChenWei Hang MaJun FanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51224 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xuan Zhang Ya Ping Huang Hai Nv Gao Mei Fang Yang Hong Zhao Jian Hua Hu Xiao Ming Chen Wei Hang Ma Jun Fan Quantification of cytomegalovirus glycoprotein Bn DNA in hematopoietic stem cell transplant recipients by real-time PCR.
description	Based on sequence variation in the N-terminus of the UL55 gene, which encodes glycoprotein B (gB), human cytomegalovirus (CMV) can be classified into four gBn genotypes. We assessed the distribution of CMV gBn genotypes and the correlation between CMV gBn DNA (detected by real-time PCR) and CMV-positive pp65 cells (identified by immunohistochemical staining) in a cohort of hematopoietic stem cell transplant patients. The distribution of gB genotypes was as follows: gBn1, 60% of patients; gBn2, 13.3%; mixed gBn1 and gBn3 infection, 26.7%; and gBn4 and other mixed infections, 0%. CMV gBn1 was the most common genotype. The detected level of CMV gB DNA correlated well with the number of CMV-positive pp65 cells detected by immunostaining (r = 0.585).
format	article
author	Xuan Zhang Ya Ping Huang Hai Nv Gao Mei Fang Yang Hong Zhao Jian Hua Hu Xiao Ming Chen Wei Hang Ma Jun Fan
author_facet	Xuan Zhang Ya Ping Huang Hai Nv Gao Mei Fang Yang Hong Zhao Jian Hua Hu Xiao Ming Chen Wei Hang Ma Jun Fan
author_sort	Xuan Zhang
title	Quantification of cytomegalovirus glycoprotein Bn DNA in hematopoietic stem cell transplant recipients by real-time PCR.
title_short	Quantification of cytomegalovirus glycoprotein Bn DNA in hematopoietic stem cell transplant recipients by real-time PCR.
title_full	Quantification of cytomegalovirus glycoprotein Bn DNA in hematopoietic stem cell transplant recipients by real-time PCR.
title_fullStr	Quantification of cytomegalovirus glycoprotein Bn DNA in hematopoietic stem cell transplant recipients by real-time PCR.
title_full_unstemmed	Quantification of cytomegalovirus glycoprotein Bn DNA in hematopoietic stem cell transplant recipients by real-time PCR.
title_sort	quantification of cytomegalovirus glycoprotein bn dna in hematopoietic stem cell transplant recipients by real-time pcr.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a0b9982ba40f4886b43afa8bc2d1ec11
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Quantification of cytomegalovirus glycoprotein Bn DNA in hematopoietic stem cell transplant recipients by real-time PCR.

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