A Genome-Wide Identification of the WRKY Family Genes and a Survey of Potential WRKY Target Genes in Dendrobium officinale

Abstract The WRKY family, one of the largest families of transcription factors, plays important roles in the regulation of various biological processes, including growth, development and stress responses in plants. In the present study, 63 DoWRKY genes were identified from the Dendrobium officinale...

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Autores principales:	Chunmei He, Jaime A. Teixeira da Silva, Jianwen Tan, Jianxia Zhang, Xiaoping Pan, Mingzhi Li, Jianping Luo, Jun Duan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a0c0d5e82d0a465496b2deb258929be5
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spelling	oai:doaj.org-article:a0c0d5e82d0a465496b2deb258929be52021-12-02T11:40:23ZA Genome-Wide Identification of the WRKY Family Genes and a Survey of Potential WRKY Target Genes in Dendrobium officinale10.1038/s41598-017-07872-82045-2322https://doaj.org/article/a0c0d5e82d0a465496b2deb258929be52017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07872-8https://doaj.org/toc/2045-2322Abstract The WRKY family, one of the largest families of transcription factors, plays important roles in the regulation of various biological processes, including growth, development and stress responses in plants. In the present study, 63 DoWRKY genes were identified from the Dendrobium officinale genome. These were classified into groups I, II, III and a non-group, each with 14, 28, 10 and 11 members, respectively. ABA-responsive, sulfur-responsive and low temperature-responsive elements were identified in the 1-k upstream regulatory region of DoWRKY genes. Subsequently, the expression of the 63 DoWRKY genes under cold stress was assessed, and the expression profiles of a large number of these genes were regulated by low temperature in roots and stems. To further understand the regulatory mechanism of DoWRKY genes in biological processes, potential WRKY target genes were investigated. Among them, most stress-related genes contained multiple W-box elements in their promoters. In addition, the genes involved in polysaccharide synthesis and hydrolysis contained W-box elements in their 1-k upstream regulatory regions, suggesting that DoWRKY genes may play a role in polysaccharide metabolism. These results provide a basis for investigating the function of WRKY genes and help to understand the downstream regulation network in plants within the Orchidaceae.Chunmei HeJaime A. Teixeira da SilvaJianwen TanJianxia ZhangXiaoping PanMingzhi LiJianping LuoJun DuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chunmei He Jaime A. Teixeira da Silva Jianwen Tan Jianxia Zhang Xiaoping Pan Mingzhi Li Jianping Luo Jun Duan A Genome-Wide Identification of the WRKY Family Genes and a Survey of Potential WRKY Target Genes in Dendrobium officinale
description	Abstract The WRKY family, one of the largest families of transcription factors, plays important roles in the regulation of various biological processes, including growth, development and stress responses in plants. In the present study, 63 DoWRKY genes were identified from the Dendrobium officinale genome. These were classified into groups I, II, III and a non-group, each with 14, 28, 10 and 11 members, respectively. ABA-responsive, sulfur-responsive and low temperature-responsive elements were identified in the 1-k upstream regulatory region of DoWRKY genes. Subsequently, the expression of the 63 DoWRKY genes under cold stress was assessed, and the expression profiles of a large number of these genes were regulated by low temperature in roots and stems. To further understand the regulatory mechanism of DoWRKY genes in biological processes, potential WRKY target genes were investigated. Among them, most stress-related genes contained multiple W-box elements in their promoters. In addition, the genes involved in polysaccharide synthesis and hydrolysis contained W-box elements in their 1-k upstream regulatory regions, suggesting that DoWRKY genes may play a role in polysaccharide metabolism. These results provide a basis for investigating the function of WRKY genes and help to understand the downstream regulation network in plants within the Orchidaceae.
format	article
author	Chunmei He Jaime A. Teixeira da Silva Jianwen Tan Jianxia Zhang Xiaoping Pan Mingzhi Li Jianping Luo Jun Duan
author_facet	Chunmei He Jaime A. Teixeira da Silva Jianwen Tan Jianxia Zhang Xiaoping Pan Mingzhi Li Jianping Luo Jun Duan
author_sort	Chunmei He
title	A Genome-Wide Identification of the WRKY Family Genes and a Survey of Potential WRKY Target Genes in Dendrobium officinale
title_short	A Genome-Wide Identification of the WRKY Family Genes and a Survey of Potential WRKY Target Genes in Dendrobium officinale
title_full	A Genome-Wide Identification of the WRKY Family Genes and a Survey of Potential WRKY Target Genes in Dendrobium officinale
title_fullStr	A Genome-Wide Identification of the WRKY Family Genes and a Survey of Potential WRKY Target Genes in Dendrobium officinale
title_full_unstemmed	A Genome-Wide Identification of the WRKY Family Genes and a Survey of Potential WRKY Target Genes in Dendrobium officinale
title_sort	genome-wide identification of the wrky family genes and a survey of potential wrky target genes in dendrobium officinale
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a0c0d5e82d0a465496b2deb258929be5
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A Genome-Wide Identification of the WRKY Family Genes and a Survey of Potential WRKY Target Genes in Dendrobium officinale

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