Immunotargeting of collagenase on thrombus
Yi-ling Lu, Ben-xia Yang, Zong-ning YinKey Laboratory of Drug Targeting and Delivery System, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People’s Republic of ChinaAbstract: In this study, we aimed to develop a thrombus-targeting delivery system of collagenase b...
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Dove Medical Press
2010
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oai:doaj.org-article:a0c1887061be4037ad73766f0232544d2021-12-02T03:05:26ZImmunotargeting of collagenase on thrombus1176-91141178-2013https://doaj.org/article/a0c1887061be4037ad73766f0232544d2010-11-01T00:00:00Zhttp://www.dovepress.com/immunotargeting-of-collagenase-on-thrombus-a5654https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yi-ling Lu, Ben-xia Yang, Zong-ning YinKey Laboratory of Drug Targeting and Delivery System, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People’s Republic of ChinaAbstract: In this study, we aimed to develop a thrombus-targeting delivery system of collagenase bound to a monoclonal antibody, and to investigate the thrombolysis of an immune-conjugate in vitro and in vivo as well as the targeting effect. We prepared the immunizing conjugation of collagenase by the 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDCI) method. In order to conjugate collagenase and a monoclonal antibody, bovine serum albumin was used as a linker, increasing the number of collagenase molecules carried and keeping collagenase and the monoclonal antibody active. In vitro thrombolysis experiments showed that collagenase had a strong dissolving effect on collagen-embolus within 24 hours. We established a rabbit pulmonary embolism model to investigate the thrombolysis effect of collagenase and collagenase immunizing conjugation in vivo. Our results revealed a significant difference between collagenase and collagenase immunizing conjugation (P < 0.05). We also established a rabbit ear edge vein model to investigate the active target of collagenase immunizing conjugation. We found that collagenase immunizing conjugation had active targets, and had a strong ability to dissolve organized thrombi. In conclusion, the thrombus-targeting delivery system of collagenase we developed has active targeting effects on thrombi.Keywords: thrombus-targeting, collagenase, monoclonal antibody Yi-ling LuBen-xia YangZong-ning YinDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 973-982 (2010) |
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DOAJ |
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DOAJ |
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EN |
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Medicine (General) R5-920 |
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Medicine (General) R5-920 Yi-ling Lu Ben-xia Yang Zong-ning Yin Immunotargeting of collagenase on thrombus |
| description |
Yi-ling Lu, Ben-xia Yang, Zong-ning YinKey Laboratory of Drug Targeting and Delivery System, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People’s Republic of ChinaAbstract: In this study, we aimed to develop a thrombus-targeting delivery system of collagenase bound to a monoclonal antibody, and to investigate the thrombolysis of an immune-conjugate in vitro and in vivo as well as the targeting effect. We prepared the immunizing conjugation of collagenase by the 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDCI) method. In order to conjugate collagenase and a monoclonal antibody, bovine serum albumin was used as a linker, increasing the number of collagenase molecules carried and keeping collagenase and the monoclonal antibody active. In vitro thrombolysis experiments showed that collagenase had a strong dissolving effect on collagen-embolus within 24 hours. We established a rabbit pulmonary embolism model to investigate the thrombolysis effect of collagenase and collagenase immunizing conjugation in vivo. Our results revealed a significant difference between collagenase and collagenase immunizing conjugation (P < 0.05). We also established a rabbit ear edge vein model to investigate the active target of collagenase immunizing conjugation. We found that collagenase immunizing conjugation had active targets, and had a strong ability to dissolve organized thrombi. In conclusion, the thrombus-targeting delivery system of collagenase we developed has active targeting effects on thrombi.Keywords: thrombus-targeting, collagenase, monoclonal antibody |
| format |
article |
| author |
Yi-ling Lu Ben-xia Yang Zong-ning Yin |
| author_facet |
Yi-ling Lu Ben-xia Yang Zong-ning Yin |
| author_sort |
Yi-ling Lu |
| title |
Immunotargeting of collagenase on thrombus |
| title_short |
Immunotargeting of collagenase on thrombus |
| title_full |
Immunotargeting of collagenase on thrombus |
| title_fullStr |
Immunotargeting of collagenase on thrombus |
| title_full_unstemmed |
Immunotargeting of collagenase on thrombus |
| title_sort |
immunotargeting of collagenase on thrombus |
| publisher |
Dove Medical Press |
| publishDate |
2010 |
| url |
https://doaj.org/article/a0c1887061be4037ad73766f0232544d |
| work_keys_str_mv |
AT yilinglu immunotargetingofcollagenaseonthrombus AT benxiayang immunotargetingofcollagenaseonthrombus AT zongningyin immunotargetingofcollagenaseonthrombus |
| _version_ |
1718401999431532544 |