A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method

Guolin Hong,1,2,* Yinhuan Liu,1,* Wei Chen,3,5 Shaohuang Weng,3,5 Qicai Liu,4 Ailin Liu,3,5 Daoxin Zheng,1 Xinhua Lin,3,51Department of Laboratory Medicine, The Fuzhou Second Affiliated Hospital of Xiamen University, Fuzhou, China; 2Department of Laboratory Medicine, Medical Technology and Engineeri...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hong GL, Liu YH, Chen W, Weng SH, Liu QC, Liu AL, Zheng DX, Lin XH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a0ca1c59dc9944b0ac7755f43d386ef1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a0ca1c59dc9944b0ac7755f43d386ef1
record_format	dspace
spelling	oai:doaj.org-article:a0ca1c59dc9944b0ac7755f43d386ef12021-12-02T09:08:48ZA sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method1176-91141178-2013https://doaj.org/article/a0ca1c59dc9944b0ac7755f43d386ef12012-09-01T00:00:00Zhttp://www.dovepress.com/a-sandwich-type-dna-electrochemical-biosensor-for-hairpin-stem-loop-st-a10999https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Guolin Hong,1,2,* Yinhuan Liu,1,* Wei Chen,3,5 Shaohuang Weng,3,5 Qicai Liu,4 Ailin Liu,3,5 Daoxin Zheng,1 Xinhua Lin,3,51Department of Laboratory Medicine, The Fuzhou Second Affiliated Hospital of Xiamen University, Fuzhou, China; 2Department of Laboratory Medicine, Medical Technology and Engineering College of Fujian Medical University, Fuzhou, China; 3Department of Pharmaceutical Analysis, Pharmacy College of Fujian Medical University, Fuzhou, China; 4Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China; 5Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, China*Guolin Hong and Yinhuan Liu contributed equally to the present studyAbstract: A highly sensitive and selective method for amplified electrochemical detection for hairpin-stem-loop structured target sequences was developed based on the temperature regulation of DNA hybrids on a sandwich-type electrochemical DNA sensor. Multistep hybridization was applied to promote the hybridization efficiency of each section of sandwich structure. The results showed that both multistep and temperature-controlling hybridization techniques were both especially made to fabricate the sensor for the tendency of internal hybridization of target gene sequences. This strategy provides significantly enhanced hybridization efficiency and sequence specificity of electrochemical detection.Keywords: sandwich-type biosensor, structured target, multistep temperature-controlling, DNA hybridization, tubercle bacillusHong GLLiu YHChen WWeng SHLiu QCLiu ALZheng DXLin XHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4953-4960 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Hong GL Liu YH Chen W Weng SH Liu QC Liu AL Zheng DX Lin XH A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
description	Guolin Hong,1,2,* Yinhuan Liu,1,* Wei Chen,3,5 Shaohuang Weng,3,5 Qicai Liu,4 Ailin Liu,3,5 Daoxin Zheng,1 Xinhua Lin,3,51Department of Laboratory Medicine, The Fuzhou Second Affiliated Hospital of Xiamen University, Fuzhou, China; 2Department of Laboratory Medicine, Medical Technology and Engineering College of Fujian Medical University, Fuzhou, China; 3Department of Pharmaceutical Analysis, Pharmacy College of Fujian Medical University, Fuzhou, China; 4Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China; 5Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, China*Guolin Hong and Yinhuan Liu contributed equally to the present studyAbstract: A highly sensitive and selective method for amplified electrochemical detection for hairpin-stem-loop structured target sequences was developed based on the temperature regulation of DNA hybrids on a sandwich-type electrochemical DNA sensor. Multistep hybridization was applied to promote the hybridization efficiency of each section of sandwich structure. The results showed that both multistep and temperature-controlling hybridization techniques were both especially made to fabricate the sensor for the tendency of internal hybridization of target gene sequences. This strategy provides significantly enhanced hybridization efficiency and sequence specificity of electrochemical detection.Keywords: sandwich-type biosensor, structured target, multistep temperature-controlling, DNA hybridization, tubercle bacillus
format	article
author	Hong GL Liu YH Chen W Weng SH Liu QC Liu AL Zheng DX Lin XH
author_facet	Hong GL Liu YH Chen W Weng SH Liu QC Liu AL Zheng DX Lin XH
author_sort	Hong GL
title	A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
title_short	A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
title_full	A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
title_fullStr	A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
title_full_unstemmed	A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
title_sort	sandwich-type dna electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/a0ca1c59dc9944b0ac7755f43d386ef1
work_keys_str_mv	AT honggl asandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT liuyh asandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT chenw asandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT wengsh asandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT liuqc asandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT liual asandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT zhengdx asandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT linxh asandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT honggl sandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT liuyh sandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT chenw sandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT wengsh sandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT liuqc sandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT liual sandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT zhengdx sandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod AT linxh sandwichtypednaelectrochemicalbiosensorforhairpinstemloopstructurebasedonmultisteptemperaturecontrollingmethod
_version_	1718398261419573248

A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method

Ejemplares similares