Effects of Berberine on the Chondrogenic Differentiation of Embryonic Limb Skeletal Progenitors

Cristina Duarte-Olivenza, Juan Antonio Montero, Carlos Ignacio Lorda-Diez Departamento de Anatomía y Biología Celular and IDIVAL, Universidad de Cantabria, Santander, 39011, SpainCorrespondence: Carlos Ignacio Lorda-DiezDepartamento de Anatomía y Biología Celular and IDIVAL, Universidad de Cantabria...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Duarte-Olivenza C, Montero JA, Lorda-Diez CI
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	chondrogenesis osteoarthritis cartilage differentiation dna methyltransferases Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/a0cafc9a17ef4d1b88a86be3eb919d38
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a0cafc9a17ef4d1b88a86be3eb919d38
record_format	dspace
spelling	oai:doaj.org-article:a0cafc9a17ef4d1b88a86be3eb919d382021-12-02T18:09:44ZEffects of Berberine on the Chondrogenic Differentiation of Embryonic Limb Skeletal Progenitors1178-7031https://doaj.org/article/a0cafc9a17ef4d1b88a86be3eb919d382021-09-01T00:00:00Zhttps://www.dovepress.com/effects-of-berberine-on-the-chondrogenic-differentiation-of-embryonic--peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Cristina Duarte-Olivenza, Juan Antonio Montero, Carlos Ignacio Lorda-Diez Departamento de Anatomía y Biología Celular and IDIVAL, Universidad de Cantabria, Santander, 39011, SpainCorrespondence: Carlos Ignacio Lorda-DiezDepartamento de Anatomía y Biología Celular and IDIVAL, Universidad de Cantabria, Santander, 39011, SpainTel +34 942201570Fax +34 942201923Email lordaci@unican.esIntroduction: Berberine (BBR) is an isoquinoline plant alkaloid with demonstrated anti-inflammatory, anti-tumor and immunosuppressive pharmacological properties that functions via multiple signaling pathways and epigenetic modulators. Numerous studies have proposed BBR as a promising therapeutic agent for joint cartilage degeneration, and other connective tissue diseases.Purpose and Methods: This work aimed to evaluate the effects of BBR on the growth and differentiation of embryonic skeletal progenitors using the limb mesoderm micromass culture assay.Results: Our findings show that at difference of its apoptotic influence on a variety of tumor tissues, cell death was not induced in skeletal progenitors by the addition of 12 or 25 μM BBR concentration to the culture medium. Morphological and transcriptional analysis revealed dual and opposite effects of BBR treatments on chondrogenesis depending on the stage of differentiation of the cultured progenitors. At early stage of culture, BBR was a potent chondrogenic inhibitor, while chondrogenesis was intensified in treatments at advanced stages of culture. The chondrogenic promoting effect was accompanied by a moderate upregulation of gene markers of prehypertrophic cartilage, including ColXa1, alkaline phosphatase Alpl, Runx2, and Indian Hedgehog Ihh. We further observed a positive transcriptional influence of BBR in the expression of DNA methyltransferase genes, Dnmt1, Dnmt3a and Dnmt3b, suggesting a potential involvement of epigenetic factors in its effects.Conclusion: Our study uncovers a new pharmacological influence of BBR in cartilage differentiation that must be taken into account in designing clinical protocols for its employment in the treatment of cartilage degenerative diseases.Keywords: chondrogenesis, osteoarthritis, cartilage differentiation, DNA methyltransferasesDuarte-Olivenza CMontero JALorda-Diez CIDove Medical Pressarticlechondrogenesisosteoarthritiscartilage differentiationdna methyltransferasesPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 5001-5011 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chondrogenesis osteoarthritis cartilage differentiation dna methyltransferases Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	chondrogenesis osteoarthritis cartilage differentiation dna methyltransferases Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Duarte-Olivenza C Montero JA Lorda-Diez CI Effects of Berberine on the Chondrogenic Differentiation of Embryonic Limb Skeletal Progenitors
description	Cristina Duarte-Olivenza, Juan Antonio Montero, Carlos Ignacio Lorda-Diez Departamento de Anatomía y Biología Celular and IDIVAL, Universidad de Cantabria, Santander, 39011, SpainCorrespondence: Carlos Ignacio Lorda-DiezDepartamento de Anatomía y Biología Celular and IDIVAL, Universidad de Cantabria, Santander, 39011, SpainTel +34 942201570Fax +34 942201923Email lordaci@unican.esIntroduction: Berberine (BBR) is an isoquinoline plant alkaloid with demonstrated anti-inflammatory, anti-tumor and immunosuppressive pharmacological properties that functions via multiple signaling pathways and epigenetic modulators. Numerous studies have proposed BBR as a promising therapeutic agent for joint cartilage degeneration, and other connective tissue diseases.Purpose and Methods: This work aimed to evaluate the effects of BBR on the growth and differentiation of embryonic skeletal progenitors using the limb mesoderm micromass culture assay.Results: Our findings show that at difference of its apoptotic influence on a variety of tumor tissues, cell death was not induced in skeletal progenitors by the addition of 12 or 25 μM BBR concentration to the culture medium. Morphological and transcriptional analysis revealed dual and opposite effects of BBR treatments on chondrogenesis depending on the stage of differentiation of the cultured progenitors. At early stage of culture, BBR was a potent chondrogenic inhibitor, while chondrogenesis was intensified in treatments at advanced stages of culture. The chondrogenic promoting effect was accompanied by a moderate upregulation of gene markers of prehypertrophic cartilage, including ColXa1, alkaline phosphatase Alpl, Runx2, and Indian Hedgehog Ihh. We further observed a positive transcriptional influence of BBR in the expression of DNA methyltransferase genes, Dnmt1, Dnmt3a and Dnmt3b, suggesting a potential involvement of epigenetic factors in its effects.Conclusion: Our study uncovers a new pharmacological influence of BBR in cartilage differentiation that must be taken into account in designing clinical protocols for its employment in the treatment of cartilage degenerative diseases.Keywords: chondrogenesis, osteoarthritis, cartilage differentiation, DNA methyltransferases
format	article
author	Duarte-Olivenza C Montero JA Lorda-Diez CI
author_facet	Duarte-Olivenza C Montero JA Lorda-Diez CI
author_sort	Duarte-Olivenza C
title	Effects of Berberine on the Chondrogenic Differentiation of Embryonic Limb Skeletal Progenitors
title_short	Effects of Berberine on the Chondrogenic Differentiation of Embryonic Limb Skeletal Progenitors
title_full	Effects of Berberine on the Chondrogenic Differentiation of Embryonic Limb Skeletal Progenitors
title_fullStr	Effects of Berberine on the Chondrogenic Differentiation of Embryonic Limb Skeletal Progenitors
title_full_unstemmed	Effects of Berberine on the Chondrogenic Differentiation of Embryonic Limb Skeletal Progenitors
title_sort	effects of berberine on the chondrogenic differentiation of embryonic limb skeletal progenitors
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/a0cafc9a17ef4d1b88a86be3eb919d38
work_keys_str_mv	AT duarteolivenzac effectsofberberineonthechondrogenicdifferentiationofembryoniclimbskeletalprogenitors AT monteroja effectsofberberineonthechondrogenicdifferentiationofembryoniclimbskeletalprogenitors AT lordadiezci effectsofberberineonthechondrogenicdifferentiationofembryoniclimbskeletalprogenitors
_version_	1718378647645061120

Effects of Berberine on the Chondrogenic Differentiation of Embryonic Limb Skeletal Progenitors

Ejemplares similares