Investigating the Function of Adult DRG Neuron Axons Using an In Vitro Microfluidic Culture System

A critical role of the peripheral axons of nociceptors of the dorsal root ganglion (DRG) is the conduction of all-or-nothing action potentials from peripheral nerve endings to the central nervous system for the perception of noxious stimuli. Plasticity along multiple sites along the pain axis has no...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rahul Atmaramani, Srivennela Veeramachaneni, Liz Valeria Mogas, Pratik Koppikar, Bryan J. Black, Audrey Hammack, Joseph J. Pancrazio, Rafael Granja-Vazquez
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nociception dorsal root ganglia axonal stimulation maladaptive plasticity drug discovery microfluidics Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/a0cb8df00bdf4655b84585a36d724080
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a0cb8df00bdf4655b84585a36d724080
record_format	dspace
spelling	oai:doaj.org-article:a0cb8df00bdf4655b84585a36d7240802021-11-25T18:23:05ZInvestigating the Function of Adult DRG Neuron Axons Using an In Vitro Microfluidic Culture System10.3390/mi121113172072-666Xhttps://doaj.org/article/a0cb8df00bdf4655b84585a36d7240802021-10-01T00:00:00Zhttps://www.mdpi.com/2072-666X/12/11/1317https://doaj.org/toc/2072-666XA critical role of the peripheral axons of nociceptors of the dorsal root ganglion (DRG) is the conduction of all-or-nothing action potentials from peripheral nerve endings to the central nervous system for the perception of noxious stimuli. Plasticity along multiple sites along the pain axis has now been widely implicated in the maladaptive changes that occur in pathological pain states such as neuropathic and inflammatory pain. Notably, increasing evidence suggests that nociceptive axons actively participate through the local expression of ion channels, receptors, and signal transduction molecules through axonal mRNA translation machinery that is independent of the soma component. In this report, we explore the sensitization of sensory neurons through the treatment of compartmentalized axon-like structures spanning microchannels that have been treated with the cytokine IL-6 and, subsequently, capsaicin. These data demonstrate the utility of isolating DRG axon-like structures using microfluidic systems, laying the groundwork for constructing the complex in vitro models of cellular networks that are involved in pain signaling for targeted pharmacological and genetic perturbations.Rahul AtmaramaniSrivennela VeeramachaneniLiz Valeria MogasPratik KoppikarBryan J. BlackAudrey HammackJoseph J. PancrazioRafael Granja-VazquezMDPI AGarticlenociceptiondorsal root gangliaaxonal stimulationmaladaptive plasticitydrug discoverymicrofluidicsMechanical engineering and machineryTJ1-1570ENMicromachines, Vol 12, Iss 1317, p 1317 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nociception dorsal root ganglia axonal stimulation maladaptive plasticity drug discovery microfluidics Mechanical engineering and machinery TJ1-1570
spellingShingle	nociception dorsal root ganglia axonal stimulation maladaptive plasticity drug discovery microfluidics Mechanical engineering and machinery TJ1-1570 Rahul Atmaramani Srivennela Veeramachaneni Liz Valeria Mogas Pratik Koppikar Bryan J. Black Audrey Hammack Joseph J. Pancrazio Rafael Granja-Vazquez Investigating the Function of Adult DRG Neuron Axons Using an In Vitro Microfluidic Culture System
description	A critical role of the peripheral axons of nociceptors of the dorsal root ganglion (DRG) is the conduction of all-or-nothing action potentials from peripheral nerve endings to the central nervous system for the perception of noxious stimuli. Plasticity along multiple sites along the pain axis has now been widely implicated in the maladaptive changes that occur in pathological pain states such as neuropathic and inflammatory pain. Notably, increasing evidence suggests that nociceptive axons actively participate through the local expression of ion channels, receptors, and signal transduction molecules through axonal mRNA translation machinery that is independent of the soma component. In this report, we explore the sensitization of sensory neurons through the treatment of compartmentalized axon-like structures spanning microchannels that have been treated with the cytokine IL-6 and, subsequently, capsaicin. These data demonstrate the utility of isolating DRG axon-like structures using microfluidic systems, laying the groundwork for constructing the complex in vitro models of cellular networks that are involved in pain signaling for targeted pharmacological and genetic perturbations.
format	article
author	Rahul Atmaramani Srivennela Veeramachaneni Liz Valeria Mogas Pratik Koppikar Bryan J. Black Audrey Hammack Joseph J. Pancrazio Rafael Granja-Vazquez
author_facet	Rahul Atmaramani Srivennela Veeramachaneni Liz Valeria Mogas Pratik Koppikar Bryan J. Black Audrey Hammack Joseph J. Pancrazio Rafael Granja-Vazquez
author_sort	Rahul Atmaramani
title	Investigating the Function of Adult DRG Neuron Axons Using an In Vitro Microfluidic Culture System
title_short	Investigating the Function of Adult DRG Neuron Axons Using an In Vitro Microfluidic Culture System
title_full	Investigating the Function of Adult DRG Neuron Axons Using an In Vitro Microfluidic Culture System
title_fullStr	Investigating the Function of Adult DRG Neuron Axons Using an In Vitro Microfluidic Culture System
title_full_unstemmed	Investigating the Function of Adult DRG Neuron Axons Using an In Vitro Microfluidic Culture System
title_sort	investigating the function of adult drg neuron axons using an in vitro microfluidic culture system
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a0cb8df00bdf4655b84585a36d724080
work_keys_str_mv	AT rahulatmaramani investigatingthefunctionofadultdrgneuronaxonsusinganinvitromicrofluidicculturesystem AT srivennelaveeramachaneni investigatingthefunctionofadultdrgneuronaxonsusinganinvitromicrofluidicculturesystem AT lizvaleriamogas investigatingthefunctionofadultdrgneuronaxonsusinganinvitromicrofluidicculturesystem AT pratikkoppikar investigatingthefunctionofadultdrgneuronaxonsusinganinvitromicrofluidicculturesystem AT bryanjblack investigatingthefunctionofadultdrgneuronaxonsusinganinvitromicrofluidicculturesystem AT audreyhammack investigatingthefunctionofadultdrgneuronaxonsusinganinvitromicrofluidicculturesystem AT josephjpancrazio investigatingthefunctionofadultdrgneuronaxonsusinganinvitromicrofluidicculturesystem AT rafaelgranjavazquez investigatingthefunctionofadultdrgneuronaxonsusinganinvitromicrofluidicculturesystem
_version_	1718411276531531776

Investigating the Function of Adult DRG Neuron Axons Using an In Vitro Microfluidic Culture System

Ejemplares similares