Ectopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits

Seedless fruits are favorable in the market because of their ease of manipulation. Sucrose transporters (SUTs or SUCs) are essential for carbohydrate metabolism in plants. Whether SUTs participate directly in causing stenospermocarpy, thereby increasing fruit quality, remains unclear. Three SUTs, na...

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Autores principales:	Yumeng Cai, Ling Yin, Wenrui Tu, Zhefang Deng, Jing Yan, Wenjie Dong, Han Gao, Jinxu Xu, Nan Zhang, Jie Wang, Lei Zhu, Qingyong Meng, Yali Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Vitis vinifera VvSUC overexpression tomato stenospermocarpy transcriptomic analysis Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/a0da09932de840d59b8a329de96ed159
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spelling	oai:doaj.org-article:a0da09932de840d59b8a329de96ed1592021-11-17T04:56:03ZEctopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits1664-462X10.3389/fpls.2021.759047https://doaj.org/article/a0da09932de840d59b8a329de96ed1592021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.759047/fullhttps://doaj.org/toc/1664-462XSeedless fruits are favorable in the market because of their ease of manipulation. Sucrose transporters (SUTs or SUCs) are essential for carbohydrate metabolism in plants. Whether SUTs participate directly in causing stenospermocarpy, thereby increasing fruit quality, remains unclear. Three SUTs, namely, VvSUC11, VvSUC12, and VvSUC27 from Vitis vinifera, were characterized and ectopic expression in tomatoes. VvSUC11- and VvSUC12-overexpressing lines had similar flower and fruit phenotypes compared with those of the wild type. VvSUC27-overexpressing lines produced longer petals and pistils, an abnormal stigma, much less and shrunken pollen, and firmer seedless fruits. Moreover, produced fruits from all VvSUC-overexpressing lines had a higher soluble solid content and sugar concentration. Transcriptomic analysis revealed more genes associated with carbohydrate metabolism and sugar transport and showed downregulation of auxin- and ethylene-related signaling pathways during early fruit development in VvSUC27-overexpressing lines relative to that of the wild type. Our findings demonstrated that stenospermocarpy can be induced by overexpression of VvSUC27 through a consequential reduction in nutrient delivery to pollen at anthesis, with a subsequent downregulation of the genes involved in carbohydrate metabolism and hormone signaling. These commercially desirable results provide a new strategy for bioengineering stenospermocarpy in tomatoes and in other fruit plants.Yumeng CaiYumeng CaiLing YinWenrui TuZhefang DengJing YanWenjie DongHan GaoJinxu XuNan ZhangJie WangLei ZhuQingyong MengYali ZhangFrontiers Media S.A.articleVitis viniferaVvSUCoverexpressiontomatostenospermocarpytranscriptomic analysisPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Vitis vinifera VvSUC overexpression tomato stenospermocarpy transcriptomic analysis Plant culture SB1-1110
spellingShingle	Vitis vinifera VvSUC overexpression tomato stenospermocarpy transcriptomic analysis Plant culture SB1-1110 Yumeng Cai Yumeng Cai Ling Yin Wenrui Tu Zhefang Deng Jing Yan Wenjie Dong Han Gao Jinxu Xu Nan Zhang Jie Wang Lei Zhu Qingyong Meng Yali Zhang Ectopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits
description	Seedless fruits are favorable in the market because of their ease of manipulation. Sucrose transporters (SUTs or SUCs) are essential for carbohydrate metabolism in plants. Whether SUTs participate directly in causing stenospermocarpy, thereby increasing fruit quality, remains unclear. Three SUTs, namely, VvSUC11, VvSUC12, and VvSUC27 from Vitis vinifera, were characterized and ectopic expression in tomatoes. VvSUC11- and VvSUC12-overexpressing lines had similar flower and fruit phenotypes compared with those of the wild type. VvSUC27-overexpressing lines produced longer petals and pistils, an abnormal stigma, much less and shrunken pollen, and firmer seedless fruits. Moreover, produced fruits from all VvSUC-overexpressing lines had a higher soluble solid content and sugar concentration. Transcriptomic analysis revealed more genes associated with carbohydrate metabolism and sugar transport and showed downregulation of auxin- and ethylene-related signaling pathways during early fruit development in VvSUC27-overexpressing lines relative to that of the wild type. Our findings demonstrated that stenospermocarpy can be induced by overexpression of VvSUC27 through a consequential reduction in nutrient delivery to pollen at anthesis, with a subsequent downregulation of the genes involved in carbohydrate metabolism and hormone signaling. These commercially desirable results provide a new strategy for bioengineering stenospermocarpy in tomatoes and in other fruit plants.
format	article
author	Yumeng Cai Yumeng Cai Ling Yin Wenrui Tu Zhefang Deng Jing Yan Wenjie Dong Han Gao Jinxu Xu Nan Zhang Jie Wang Lei Zhu Qingyong Meng Yali Zhang
author_facet	Yumeng Cai Yumeng Cai Ling Yin Wenrui Tu Zhefang Deng Jing Yan Wenjie Dong Han Gao Jinxu Xu Nan Zhang Jie Wang Lei Zhu Qingyong Meng Yali Zhang
author_sort	Yumeng Cai
title	Ectopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits
title_short	Ectopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits
title_full	Ectopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits
title_fullStr	Ectopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits
title_full_unstemmed	Ectopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits
title_sort	ectopic expression of vvsuc27 induces stenospermocarpy and sugar accumulation in tomato fruits
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a0da09932de840d59b8a329de96ed159
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Ectopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits

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