Visualizing group II intron dynamics between the first and second steps of splicing

Group II introns are self-splicing ribozymes. Here, the authors employ enzymatic assay, X-ray crystallography and molecular dynamics simulations to show that protonation of the group II intron catalytic triad plays an important role for the transition from the first to the second step of splicing.

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Autores principales:	Jacopo Manigrasso, Isabel Chillón, Vito Genna, Pietro Vidossich, Srinivas Somarowthu, Anna Marie Pyle, Marco De Vivo, Marco Marcia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a0ddbc7f74af45d393f62a1504d55452
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spelling	oai:doaj.org-article:a0ddbc7f74af45d393f62a1504d554522021-12-02T15:03:03ZVisualizing group II intron dynamics between the first and second steps of splicing10.1038/s41467-020-16741-42041-1723https://doaj.org/article/a0ddbc7f74af45d393f62a1504d554522020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16741-4https://doaj.org/toc/2041-1723Group II introns are self-splicing ribozymes. Here, the authors employ enzymatic assay, X-ray crystallography and molecular dynamics simulations to show that protonation of the group II intron catalytic triad plays an important role for the transition from the first to the second step of splicing.Jacopo ManigrassoIsabel ChillónVito GennaPietro VidossichSrinivas SomarowthuAnna Marie PyleMarco De VivoMarco MarciaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jacopo Manigrasso Isabel Chillón Vito Genna Pietro Vidossich Srinivas Somarowthu Anna Marie Pyle Marco De Vivo Marco Marcia Visualizing group II intron dynamics between the first and second steps of splicing
description	Group II introns are self-splicing ribozymes. Here, the authors employ enzymatic assay, X-ray crystallography and molecular dynamics simulations to show that protonation of the group II intron catalytic triad plays an important role for the transition from the first to the second step of splicing.
format	article
author	Jacopo Manigrasso Isabel Chillón Vito Genna Pietro Vidossich Srinivas Somarowthu Anna Marie Pyle Marco De Vivo Marco Marcia
author_facet	Jacopo Manigrasso Isabel Chillón Vito Genna Pietro Vidossich Srinivas Somarowthu Anna Marie Pyle Marco De Vivo Marco Marcia
author_sort	Jacopo Manigrasso
title	Visualizing group II intron dynamics between the first and second steps of splicing
title_short	Visualizing group II intron dynamics between the first and second steps of splicing
title_full	Visualizing group II intron dynamics between the first and second steps of splicing
title_fullStr	Visualizing group II intron dynamics between the first and second steps of splicing
title_full_unstemmed	Visualizing group II intron dynamics between the first and second steps of splicing
title_sort	visualizing group ii intron dynamics between the first and second steps of splicing
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/a0ddbc7f74af45d393f62a1504d55452
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Visualizing group II intron dynamics between the first and second steps of splicing

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